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  4. Rat pigment epithelium-derived factor,PEDF ELISA Kit

Rat pigment epithelium-derived factor,PEDF ELISA Kit

  • 中文名稱：
    大鼠色素上皮衍生因子(PEDF)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E08819r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠色素上皮衍生因子(PEDF)酶聯(lián)免疫試劑盒（CSB-E08819r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的PEDF含量。PEDF（色素上皮衍生因子）是一種具有多種生物功能的分泌性糖蛋白，主要在視網(wǎng)膜色素上皮細(xì)胞中表達(dá)。它具有抗血管生成、抗炎和神經(jīng)營養(yǎng)作用，對多種腫瘤細(xì)胞具有抑制作用。其研究機(jī)制涉及通過調(diào)節(jié)細(xì)胞增殖、凋亡和遷移等途徑抑制腫瘤生長。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，適用于體外培養(yǎng)細(xì)胞分泌功能評估、疾病模型動物血清標(biāo)志物分析以及組織微環(huán)境中PEDF動態(tài)變化的科研探索，為血管生成調(diào)控、氧化應(yīng)激機(jī)制及神經(jīng)退行性疾病等基礎(chǔ)研究提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    PEDF
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    6.25 pg/mL-400 pg/mL
  • 靈敏度：
    1.56 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cancer
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat PEDF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1000Average %87
    Range %81-92
    1:2000Average %95
    Range %91-99
    1:4000Average %92
    Range %85-97
    1:8000Average %96
    Range %91-102
  • 回收率：
    The recovery of rat PEDFspiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9289-95
    EDTA plasma (n=4)8783-91
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    4002.758 2.615 2.687 2.549
    2002.065 1.968 2.017 1.879
    1001.357 1.397 1.377 1.239
    500.846 0.831 0.839 0.701
    250.572 0.581 0.577 0.439
    12.50.354 0.334 0.344 0.206
    6.250.264 0.256 0.260 0.122
    00.139 0.137 0.138
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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相關(guān)問答

■ 常見問題解答
Q:

Please provide the following information regarding Rat pigment epithelium-derived factor, PEDF ELISA Kit (CSB-E08819r):
a. Can cell culture lysates/supernatants samples be used with this kit? Have cell culture lysates/supernatants samples been tested with this kit?
b. If yes, what were the observed concentration values?
c. If they have not, would this assay be compatible with cell culture lysates/supernatants samples? Is there a recommended protocol to use with cell culture lysates/supernatants samples?

A:
Thanks for your inquiry.
The kit can be used for cell culture supernatants matrix.
However, we have no detection on the sample. If the sample content is under the detection range, it can be detected well.