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  4. Rat prealbumin,PA ELISA Kit

Rat prealbumin,PA ELISA Kit

  • 中文名稱：
    大鼠前白蛋白(PA)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E13251r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠前白蛋白(PA)酶聯(lián)免疫試劑盒（CSB-E13251r）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的PA含量。試劑盒檢測范圍為375 ng/mL-6000 ng/mL，在肝損傷模型、營養(yǎng)干預(yù)研究及相關(guān)疾病機(jī)制探索中具有重要科研價值；可用于評估肝細(xì)胞功能、監(jiān)測培養(yǎng)體系中蛋白質(zhì)分泌動態(tài)，或分析組織樣本病理生理變化；滿足基礎(chǔ)研究中對PA定量分析的需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Ttr ELISA Kit; Tt ELISA Kit; Transthyretin ELISA Kit; Prealbumin ELISA Kit; TBPA ELISA Kit
  • 縮寫：
    PA
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    375 ng/mL-6000 ng/mL
  • 靈敏度：
    375 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat PA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:200 Average % 95  
    Range % 91-99  
    1:400 Average % 103  
    Range % 99-107  
    1:800 Average % 98  
    Range % 95-103  
    1:1600 Average % 93  
    Range % 87-97  
  • 回收率：
    The recovery of rat PA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 99 95-103  
    EDTA plasma (n=4) 89 82-97  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    6000 0.152 0.163 0.158    
    3000 0.288 0.300 0.294    
    1500 0.561 0.568 0.565    
    750 0.903 0.925 0.914    
    375 1.477 1.434 1.456    
    0 2.662 2.591 2.627    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.
  • 基因功能參考文獻(xiàn)：
    1. Data suggest that the mutated transthyretin (TTR) seems to have potential as biomarker for early detection of hepatocellular carcinoma (HCC). PMID: 28876464
    2. Serum transthyretin level dramatically decreased in animals displaying decompression sickness symptoms. PMID: 27068327
    3. We conclude that stress upregulates TTR expression in choroid plexus. PMID: 22371232
    4. Decreased trophoblast expression of retinol transport proteins suggest that nitrofen may interfere with the fetal retinol transport resulting in reduced pulmonary RBP and TTR levels and causing pulmonary hypoplasia in CDH. PMID: 22015466
    5. Data demonstrate that, in vitro and in vivo, transthyretin is up-regulated by progesterone, and suggest that this response is mediated through a progesterone receptor-mediated mechanism. PMID: 20535645
    6. Structural basis of negative cooperativity in transthyretin PMID: 11995997
    7. Complex of rat transthyretin with tetraiodothyroacetic acid refined at 2.1 and 1.8 A resolution PMID: 11995998
    8. Comparison of binding interactions of dibromoflavonoids with transthyretin PMID: 11995999
    9. Amyloid formation in rat transthyretin: effect of oxidative stress PMID: 12135814
    10. data show the LR1 gene is up-regulated following short interval successive partial hepatectomy; it may play an important role in liver regeneration PMID: 12508371
    11. Data suggest that monomeric transthyretin may represent an ischemia-specific CSF marker to indicate the sequential changes according to ischemic insults of the brain. PMID: 15469881
    12. Our study provides genetic, behavioural and molecular evidence that TTR is involved in the maintenance of normal cognitive processes during aging by acting on the retinoid signalling pathway. PMID: 17512093
    13. Ttr mRNA expression in dorsal root ganglia and cauda equina. PMID: 18406527
    14. Sex hormones may increase TTR synthesis by the liver, and therefore, may contribute to amyloid deposition. PMID: 18568387
    15. Results suggest that transthyretin is up-regulated by estradiol via a pathway involving estrogen receptors alpha and beta. PMID: 19130215
    16. The expression of transthyretin in brain of a transgenic mouse model of Alzheimer's disease is asymmetric and affected by age and sex. PMID: 19167467
    17. rat and murine TTR have a lower intrinsic beta-aggregation propensity and a similar native beta-structure stability compared with human TTR. PMID: 19602727
    18. Transthyretin is expressed in the pineal gland >30-fold higher than the median expression level in other tissues, at levels similar to those in the choroid plexus. PMID: 19103603

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Transthyretin family
  • 組織特異性：
    Detected in serum and cerebrospinal fluid (at protein level). Highly expressed in the choroid plexus. Detected at lower levels in the liver.
  • 數(shù)據(jù)庫鏈接：

    KEGG: rno:24856

    STRING: 10116.ENSRNOP00000022113

    UniGene: Rn.1404