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  4. Rat prolactin,PRL ELISA Kit

Rat prolactin,PRL ELISA Kit

  • 中文名稱：
    大鼠催乳素(PRL)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E06881r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠催乳素(PRL)酶聯(lián)免疫試劑盒（CSB-E06881r）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿、細(xì)胞裂解物樣本中的PRL含量。PRL（催乳素）是一種由垂體前葉分泌的蛋白質(zhì)激素，主要作用是促進乳腺發(fā)育和泌乳。研究發(fā)現(xiàn)，PRL及其受體PRLR在多種腫瘤中高表達，并促進腫瘤細(xì)胞增殖、血管生成和抵抗化療。PRL-PRLR通路在乳腺癌、前列腺癌和膠質(zhì)母細(xì)胞瘤等腫瘤中發(fā)揮作用，成為潛在的治療靶點。試劑盒檢測范圍為0.125 ng/mL-50 ng/mL，適用于探究哺乳期動物模型、激素代謝異常機制、生殖生物學(xué)研究以及乳腺發(fā)育相關(guān)課題，特別在評估藥物干預(yù)、環(huán)境因素或基因修飾對PRL分泌水平的影響實驗中具有重要應(yīng)用價值本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Prl ELISA Kit; Prolactin ELISA Kit; PRL ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates, cell lysates
  • 檢測范圍：
    0.125 ng/mL-50 ng/mL
  • 靈敏度：
    0.125 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 90-105  
    1:2 Average % 94  
    Range % 91-105  
    1:4 Average % 95  
    Range % 90-101  
    1:8 Average % 97  
    Range % 92-104  
  • 回收率：
    The recovery of rat PRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 87-97  
    EDTA plasma (n=4) 97 90-105  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    50 0.147 0.146 0.147    
    9.37 0.305 0.314 0.310    
    1.56 0.512 0.523 0.518    
    0.44 0.865 0.887 0.876    
    0.125 1.411 1.424 1.418    
    0 2.118 2.122 2.120    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻

產(chǎn)品評價

平均分:
5.0分 - 2 個評價

樣品類型：血清

樣品信息：大鼠

稀釋比：沒有稀釋

產(chǎn)品評價： 抱著僥幸的心理在同事的推薦下用了華美生物的大鼠泌乳素elisa檢測試劑盒，結(jié)果發(fā)現(xiàn)做出來的血漿標(biāo)準(zhǔn)曲線R回歸的很好準(zhǔn)確度高，用來檢測計算處理組的血漿樣本有信心。

By 唐老師

樣品類型：血清

樣品信息：大鼠

稀釋比：沒有稀釋

產(chǎn)品評價： 我用華美生物的CSB-E06881r 檢測正常大鼠的血漿樣本，標(biāo)準(zhǔn)曲線的OD值在0.085-1.72之間，7周齡雌性SD大鼠血漿樣本對照組OD是0.133、0.081、0.110、0.102，依據(jù)標(biāo)準(zhǔn)曲線計算的濃度為 22.39、54.48、31.51、36.08（ng/ml）。檢測的曲線方程R方接近1，回歸的很好計算的樣本的數(shù)值可靠，強烈推薦

By 唐老師

靶點詳情

  • 功能：
    Prolactin acts primarily on the mammary gland by promoting lactation.
  • 基因功能參考文獻：
    1. It was examined whether prolactin modulation alters ventral prostate (VP) morphophysiology in adult castrated rats. PMID: 28988314
    2. Data suggest that prolactin and oleic acid synergistically stimulate beta-cell proliferation and islet growth; in cultured neonatal islets, prolactin increases cell proliferation 6-fold, oleic acid 3.5-fold, and their combination 15-fold. Similar results were observed in cultured adult islets. PMID: 28686504
    3. Involvement of prolactin in the regulation of bicarbonate biodynamics using female rat model of cholestasis of pregnancy. PMID: 28361424
    4. This study reports on the effect of copulation on potentially precancerous prostate lesions, serum testosterone and prolactin levels in rats. PMID: 27356573
    5. This study demonstrated that the Cultured neurons expressed mRNA for both PRL and its receptor (PRLR), and both PRL and PRLR expression levels changed after the excitotoxic insult. PMID: 26874070
    6. Data confirm that prolactin secretion by anterior pituitary gland can be down-regulated by environmental endocrine disruptors; here, perfluorooctane sulfonate affects periventricular-hypophysial dopaminergic neurons in mechanism involving estradiol. PMID: 26032630
    7. In conclusion, PRL was responsible for the lactation-induced mucosal adaptations, which were associated with compensatory increase in FGF-23 expression probably to prevent calcium hyperabsorption. PMID: 26657069
    8. PRL is unchanged in a rat model of panic evoked by dorsal periaqueductal gray stimulation. PMID: 25618592
    9. Prolactin actively supports lactation providing amino acids to the gland through SNAT2 for the synthesis of milk proteins. PMID: 25701231
    10. Results showed that an active sexual life, with constant execution of sexual behavior, produces a sustained increase in serum PRL, prostate PRL receptors, and the pStat3 signaling pathway PMID: 25446202
    11. This study showe that TGF-beta and TNF-alpha antagonize the effect of each other on the expression and release of prolactin in a cell line and in obese and diabetic rats. PMID: 25715833
    12. supraphysiological levels of PRL affect carcinogenesis. PL induces regression of the tumors due to the differentiation produced on the mammary cells. PMID: 25136563
    13. Prolactin induces apoptosis of lactotropes PMID: 24859278
    14. High Prolactin expression is associated with Streptozotocin diabetes. PMID: 24984282
    15. insulin activates prolactin gene transcription by activating Elk-1 that recruits the NuA4 complex to the promoter. PMID: 24075908
    16. PRL acts on ARC neurons to inhibit kisspeptin expression in female rats PMID: 24456164
    17. serum levels may be involved in the homing events to mammary glands, probably helping antibody-secreting cells and protecting the gland during lactation development PMID: 23904563
    18. Prolactin inhibits apoptosis in chondrocytes in response to a mixture of proinflammatory cytokines. PMID: 23908112
    19. Our data confirmed the regulatory role of dopamine, serotonin, and TRH on PRL secretion, however, the interaction between these and glutamatergic systems was not confirmed. PMID: 23641787
    20. Data from 2 hepatic insufficiency models suggest that pituitary expression of Prl and estrogen receptor alpha is up-regulated in this state, positively correlating with increase in serum 17beta-estradiol level; data show gastro-hepato-pituitary axis. PMID: 22843122
    21. The results indicate that under prolonged duration of a daily melatonin signal, rat anterior pituitary prolactin synthesis and release are depressed, together with significant changes in the redox and circadian mechanisms controlling them. PMID: 22891630
    22. results show that the effect of centrally injected allopreganolone over reproductive function could be due to a centrally originated LH mediated effect over ovarian function that affects luteal regression, through the inhibition of apoptosis and stimulation of progesterone and prolactin release PMID: 22674474
    23. Lactotroph nonselective cation channels, presumably belonging to the TRPC family, contribute to the background depolarizing conductance and firing of action potentials with consequent prolactin release. PMID: 22480423
    24. Data suggest that the resolution of an inflammatory response is associated with dramatic activation of the prolactin (PRL) gene promoter in the myeloid lineage. PMID: 22495675
    25. E(2) may act as a modulator of the prolactin secretory response induced by TRH through membrane estrogen receptors, with the contribution by PI3K/Akt pathway activation. PMID: 22354782
    26. The results show that PRL expression and cell proliferation are controlled in part by CEBPD. PMID: 21980073
    27. Gonadotrophs are the major source of Nrg1 in the normal anterior pituitary and Nrg1 may function as a paracrine/juxtacrine regulator of PRL secretion. PMID: 21919974
    28. Data suggest that prolactin is involved in regulation of prolactin receptor expression and maintenance of physiological cell renewal in anterior pituitary. PMID: 22094470
    29. Data suggest that inflammation leads to accumulation of endogenous prolactin in female and male rats, and that prolactin acts as an inflammatory mediator at different time points for female and intact male rats. PMID: 21777304
    30. Prolactin inhibition during late lactation programs renal function damage in adult offspring. PMID: 21823059
    31. Estradiol regulates dopamine-activataed GIRK channel activity in pituitary lactotrophs, regulating prolactin release during the estrous cycle. PMID: 21653876
    32. Integrity of the pelvic nerve is necessary for the systemic oxytocin induction of the PRL secretory rhythm in ovariectomized rats. PMID: 21677274
    33. NONO and SFPQ were functionally involved in circadian Prl transcription since overexpression of both proteins greatly reduced Prl promoter activity (P<0.001) and disrupted its circadian pattern. PMID: 21507896
    34. The anterior pituitary production of 16 kDa prolactin is variable along the estrous cycle and increased by estrogens. PMID: 21760910
    35. Complex interactions between Ahr and Esr alter Prl and luteinizing hormone (LH) synthesis by direct actions in lactotropes and gonadotropes. PMID: 21187122
    36. Results suggest that bone morphogenetic proteins elicit differential actions in the regulation of prolactin release dependent on cellular cAMP-PKA activity. PMID: 20970474
    37. PREB can function as a transcriptional regulator of PRL promoter activity and might be involved in TRH-induced PRL gene transcription PMID: 20960102
    38. Results suggest a role for dopamine in the generation of the oestrous prolactin surge. PMID: 20722974
    39. Prolactin diminishes the damaging actions of excitotoxicity in the kainate model of epilepsy. PMID: 20570717
    40. Data show that inactivation of tyrosine hydroxylase in hypothalamic neuroendocrine dopamine neurons is required for suckling-induced prolactin and ACTH responses. PMID: 20170714
    41. Results demonstrate clearly that certain circadian elements binding to the E-box133 site are required for episodes of PRL mRNA expression. PMID: 20215567
    42. Prolactin gene expression data suggest that pituitary tissue comprises a series of cell ensembles, which individually display a variety of patterns of short-term stochastic behaviour, but together yield long-range and long-term coordinated behaviour. PMID: 20130141
    43. results indicate that specific effects upon male rat lactotropes may be exerted by PRL variants released from anterior pituitary glands of lactating and non-lactating rats PMID: 19590175
    44. At 5 h after progesterone (P(4)) treatment, tyrosine hydroxylase activity and phosphorylation state declined coincident with an increase in plasma prolactin in both P(4)-treated morning and afternoon groups. PMID: 19945993
    45. PRL directly enhanced the transcellular and paracellular calcium transport in the rat cecum through the nongenomic signaling pathways involving PI3K, PKC, and ROCK. PMID: 19449156
    46. Review: evidence demonstrating PRL synthesis by different subtypes of immune cells from humans, mice and rats, describe the regulation of PRL gene expression in human lymphocytes, and discuss the functions of PRL made by immune cells. PMID: 11721692
    47. Review: Effects of prolactin on signal transduction and gene expression: possible relevance for systemic lupus erythematosus. PMID: 11721698
    48. Review: effects of changing the ratio of these two forms in maternal PRL on gamma delta T cell development in rat pups in utero PMID: 11721700
    49. physiological prolactin secretion response to stress depends on the renin--angiotensin system PMID: 11888852
    50. Inverse control of prolactin and growth hormone gene expression: effect of thyroliberin on transcription and RNA stabilization. PMID: 11892801

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Somatotropin/prolactin family
  • 數(shù)據(jù)庫鏈接：

    KEGG: rno:24683

    STRING: 10116.ENSRNOP00000023412

    UniGene: Rn.9759