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Rat prostacycline,PGI2 ELISA Kit

  • 中文名稱:
    大鼠前列環(huán)素(PGI2)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E13706r
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3900/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    大鼠前列環(huán)素(PGI2)酶聯(lián)免疫試劑盒(CSB-E13706r)為競(jìng)爭(zhēng)法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的PGI2含量。PGI2,即前列環(huán)素,是一種花生四烯酸的代謝產(chǎn)物,具有廣泛的生物學(xué)功能,如血管舒張、抗血小板聚集和免疫調(diào)節(jié)等。其研究機(jī)制涉及與特定受體結(jié)合,激活G蛋白,進(jìn)而調(diào)節(jié)細(xì)胞內(nèi)信號(hào)傳導(dǎo)。近年來(lái),PGI2在治療肺動(dòng)脈高壓等疾病中的應(yīng)用研究取得了顯著進(jìn)展。試劑盒檢測(cè)范圍為0.25 ng/mL-50 ng/mL,適用于科研領(lǐng)域中對(duì)大鼠模型血管功能研究、抗血栓藥物作用機(jī)制探索、炎癥相關(guān)疾病動(dòng)物實(shí)驗(yàn)的分子標(biāo)志物檢測(cè),以及組織微環(huán)境中PGI2代謝動(dòng)態(tài)分析,為心血管生物學(xué)、藥理學(xué)及病理機(jī)制研究提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫(xiě):
    PGI2
  • 種屬:
    Rattus norvegicus (Rat)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    0.25 ng/mL-50 ng/mL
  • 靈敏度:
    0.125 ng/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Biochemicals
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat PGI2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 96  
    Range % 92-104  
    1:2 Average % 94  
    Range % 91-98  
    1:4 Average % 102  
    Range % 98-106  
    1:8 Average % 88  
    Range % 82-94  
  • 回收率:
    The recovery of rat PGI2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 90-98  
    EDTA plasma (n=4) 103 98-107  
                 
                 
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    50 0.223 0.222 0.223    
    12.5 0.356 0.351 0.354    
    4 0.546 0.557 0.552    
    1 0.794 0.804 0.799    
    0.25 1.173 1.275 1.224    
    0 2.404 2.305 2.355    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

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