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  4. Rat relaxin 1 (RLN1) ELISA kit

Rat relaxin 1 (RLN1) ELISA kit

  • 中文名稱：
    大鼠松弛肽/松弛素1(RLN1)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL019749RA
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠松弛肽/松弛素1(RLN1)酶聯(lián)免疫試劑盒（CSB-EL019749RA）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的RLN1含量。試劑盒檢測(cè)范圍為2.4 pg/mL-150 pg/mL，適用于體外實(shí)驗(yàn)環(huán)境下研究RLN1在生殖生理、心血管疾病模型或組織再生機(jī)制中的表達(dá)變化，為探索其調(diào)控網(wǎng)絡(luò)及分子互作提供可靠數(shù)據(jù)支持本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Rln1 ELISA Kit; Rln ELISA Kit; Prorelaxin 1 [Cleaved into: Relaxin B chain; Relaxin A chain] ELISA Kit
  • 縮寫：
    RLN1
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測(cè)范圍：
    2.4 pg/mL-150 pg/mL
  • 靈敏度：
    0.6 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat RLN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %95
    Range %90-99
    1:2Average %89
    Range %84-93
    1:4Average %92
    Range %88-96
    1:8Average %97
    Range %92-101
  • 回收率：
    The recovery of rat RLN1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 105100-110
    EDTA plasma (n=4)8480-88
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    1502.643 2.487 2.565 2.439
    752.097 2.236 2.167 2.041
    37.51.468 1.551 1.510 1.384
    18.750.907 0.945 0.926 0.800
    9.40.583 0.617 0.600 0.474
    4.70.384 0.401 0.393 0.267
    2.40.235 0.252 0.244 0.118
    00.121 0.130 0.126
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals.
  • 基因功能參考文獻(xiàn)：
    1. Relaxin-1 mRNA in the hearts of diabetic rats was significantly increased and that relaxin-3 mRNA expression at different time points was decreased. In addition, significant down-regulation of the mRNA expression of RXFP1 and RXFP3 was observed at 4 weeks after Streptozocin. PMID: 28776188
    2. The digestive tract has been shown to express relaxin receptors; the hormone appears to exert site-specific effects acting at the neural or at the smooth muscle level, mainly by a nitric oxide-mediated mechanism. (Review) PMID: 28606038
    3. We have shown that relaxin stimulates Sertoli cell proliferation through the activation of MEK/ERK1/2 and PI3K/AKT pathways. PMID: 24640565
    4. The aim of the present study was to explore a role of relaxin at specific stages of spermatogenesis using a co-culture of rat Sertoli and germ cells. PMID: 24640566
    5. Raxin is a mediator of the refractoriness of the mesangial cells to angiotensin II during pregnancy. PMID: 23207895
    6. Data demonstrate a functional role for relaxin in mediating uterine artery compliance in pregnant rats, which may be necessary to maintain adequate uterine blood flow to the uterus and placenta. PMID: 22744867
    7. A novel G-quadruplex in the relaxin gene, is reported. PMID: 22363579
    8. Rln-1 was localized in the granulosa cells of the ovarian follicles, and immunoreactivity against Rln 1 was increased by orthodontic tooth movement in a time-dependent manner. PMID: 21410550
    9. relaxin regulates fibroblast proliferation, differentiation, and collagen deposition and may have therapeutic potential in diseased states characterized by cardiac fibrosis. PMID: 15155573
    10. role for relaxin in the systemic hemodynamic changes of pregnancy, as well as novel therapeutic potential for relaxin in modifying arterial stiffness and cardiac afterload. PMID: 15198972
    11. High glucose concentration could stimulate the expression of endogenous relaxin. PMID: 19542742

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Insulin family
  • 數(shù)據(jù)庫鏈接：

    KEGG: rno:25616

    STRING: 10116.ENSRNOP00000021315

    UniGene: Rn.9830