Rat secreted phospholipase A2,sPLA2 ELISA Kit
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中文名稱:大鼠分泌型磷脂酶A2(sPLA2)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-E13206r
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規(guī)格:96T/48T
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價(jià)格:¥3900/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:大鼠分泌型磷脂酶A2(sPLA2)酶聯(lián)免疫試劑盒(CSB-E13206r)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的sPLA2含量。sPLA2即分泌型磷脂酶 A2,在生物體內(nèi)廣泛存在。它參與磷脂代謝,能水解膜磷脂產(chǎn)生花生四烯酸和溶血磷脂等。其異常表達(dá)與多種疾病如炎癥、心血管病、腫瘤等相關(guān),因此針對(duì) sPLA2 作用機(jī)制的研究,能為疾病治療提供新靶點(diǎn)與思路。試劑盒檢測(cè)范圍為0.78 ng/mL-50 ng/mL,支持科研人員探究sPLA2在疾病模型中的動(dòng)態(tài)變化,例如心血管疾病機(jī)制研究、炎癥相關(guān)動(dòng)物實(shí)驗(yàn)或藥物干預(yù)效果評(píng)估,尤其適用于分析血清/血漿樣本的循環(huán)sPLA2水平或組織勻漿中的局部表達(dá)差異本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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縮寫:sPLA2
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種屬:Rattus norvegicus (Rat)
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樣本類型:serum, plasma, tissue homogenates
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檢測(cè)范圍:0.78 ng/mL-50 ng/mL
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靈敏度:0.195 ng/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Others
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat sPLA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 91 Range % 86-95 1:2 Average % 93 Range % 87-97 1:4 Average % 91 Range % 85-97 1:8 Average % 92 Range % 88-96 -
回收率:
The recovery of rat sPLA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 87 82-93 EDTA plasma (n=4) 92 87-94 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 50 2.256 2.188 2.222 2.093 25 1.633 1.509 1.571 1.442 12.5 0.998 0.970 0.984 0.855 6.25 0.564 0.553 0.559 0.430 3.12 0.367 0.352 0.360 0.231 1.56 0.242 0.232 0.237 0.108 0.78 0.186 0.180 0.183 0.054 0 0.130 0.128 0.129 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Effects of swimming on pain and inflammatory factors in rats with lumbar disc herniation Yizhuan Huang, et al,Experimental and Therapeutic Medicine,2019
- High-fructose and high-fat feeding correspondingly lead to the development of lysoPC-associated apoptotic cardiomyopathy and adrenergic signaling-related cardiac hypertrophy Jiung-Pang Huang.et al,International Journal of Cardiology,2016
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