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  4. Rat soluble E-selectin,sE-selectin ELISA Kit

Rat soluble E-selectin,sE-selectin ELISA Kit

  • 中文名稱：
    大鼠可溶性E選擇素(sE-selectin)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E07996r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠可溶性E選擇素(sE-selectin)酶聯(lián)免疫試劑盒（CSB-E07996r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的SELE含量。SELE即選擇素E，是內(nèi)皮細(xì)胞在炎性因子刺激下表達(dá)的黏附分子。其背景圍繞炎癥反應(yīng)，在多種炎癥相關(guān)疾病中表達(dá)異常。研究機(jī)制表明，它能介導(dǎo)白細(xì)胞與內(nèi)皮細(xì)胞的黏附、滾動(dòng)，參與炎癥細(xì)胞募集，在炎癥、腫瘤轉(zhuǎn)移等病理過(guò)程意義重大。試劑盒檢測(cè)范圍為78 pg/mL-5000 pg/mL，適用于心血管疾病、糖尿病并發(fā)癥等炎癥相關(guān)動(dòng)物模型的機(jī)制研究，或體外細(xì)胞實(shí)驗(yàn)中內(nèi)皮細(xì)胞激活程度的動(dòng)態(tài)監(jiān)測(cè)。為研究炎癥信號(hào)通路、藥物干預(yù)效果或組織修復(fù)機(jī)制提供可靠工具，滿足基礎(chǔ)科研中對(duì)靶標(biāo)蛋白定量分析的需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    Sele ELISA Kit; Elam-1 ELISA Kit; E-selectin ELISA Kit; CD62 antigen-like family member E ELISA Kit; Endothelial leukocyte adhesion molecule 1 ELISA Kit; ELAM-1 ELISA Kit; Leukocyte-endothelial cell adhesion molecule 2 ELISA Kit; LECAM2 ELISA Kit; CD antigen CD62E ELISA Kit
  • 縮寫(xiě)：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測(cè)范圍：
    78 pg/mL-5000 pg/mL
  • 靈敏度：
    19.5 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat sE-selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:5Average %92
    Range %88-108
    1:10Average %94
    Range %91-98
    1:20Average %96
    Range %89-100
    1:40Average %84
    Range %80-88
  • 回收率：
    The recovery of rat sE-selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9993-105
    EDTA plasma (n=4)9490-98
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    50002.746 2.645 2.696 2.542
    25001.976 1.875 1.926 1.772
    12501.228 1.204 1.216 1.062
    6250.782 0.761 0.772 0.618
    3120.522 0.511 0.517 0.363
    1560.363 0.352 0.358 0.204
    780.290 0.285 0.288 0.134
    00.154 0.153 0.154 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.
  • 基因功能參考文獻(xiàn)：
    1. The e-selectin/biliverdin reductase pathway participates in the inhibitory process of hypoxia in pulmonary artery endothelial cell apoptosis which is mediated by the mitochondrial-dependent apoptosis pathway. PMID: 27033411
    2. Decrease in uterine pinopodes and L-selectin ligand MECA-79 expression during uterine receptivity period by testosterone may cause failure of blastocyst implantation. PMID: 24966906
    3. E-selectin blockade similarly inhibits the migration of activated CCR4(negative) and CCR4(positive) CD4 T cells to dermal inflammation. PMID: 22664869
    4. The expression of E-selectin is significantly increased in lung tissue of ovariectomized rats, an effect prevented by estradiol but not by progesterone treatment. PMID: 20735828
  • 亞細(xì)胞定位：
    Cell membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Selectin/LECAM family
  • 數(shù)據(jù)庫(kù)鏈接：