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  4. Rat tartrate-resistant acid phosphatase 5b,TRACP-5b ELISA Kit

Rat tartrate-resistant acid phosphatase 5b,TRACP-5b ELISA Kit

  • 中文名稱：
    大鼠抗酒石酸酸性磷酸酶5b(TRACP-5b)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E08491r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠抗酒石酸酸性磷酸酶5b(TRACP-5b)酶聯(lián)免疫試劑盒（CSB-E08491r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的TRACP-5b含量。TRACP-5b是一種靶點(diǎn)。它與骨代謝緊密相關(guān)，在破骨細(xì)胞中高度表達(dá)。研究機(jī)制方面，主要聚焦于其在破骨細(xì)胞活化、骨吸收過(guò)程中的作用。檢測(cè)其水平變化，有助于評(píng)估骨代謝狀態(tài)和相關(guān)疾病如骨質(zhì)疏松等的發(fā)病機(jī)制。試劑盒檢測(cè)范圍為0.312 mIU/mL-20 mIU/mL，在骨質(zhì)疏松機(jī)制研究、骨骼疾病模型評(píng)估及骨代謝相關(guān)藥物開(kāi)發(fā)等領(lǐng)域具有重要科研價(jià)值；適用于體外定量分析骨代謝相關(guān)動(dòng)物模型的生物標(biāo)志物表達(dá)水平，為骨生物學(xué)研究、藥物干預(yù)效果評(píng)價(jià)及病理機(jī)制探索提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫(xiě)：
    TRACP-5b
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    0.312 mIU/mL-20 mIU/mL
  • 靈敏度：
    0.078 mIU/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat TRACP-5b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %92
    Range %89-95
    1:2Average %100
    Range %97-103
    1:4Average %93
    Range %88-97
    1:8Average %98
    Range %93-102
  • 回收率：
    The recovery of rat TRACP-5b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9490-96
    EDTA plasma (n=4)9591-97
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mIU/mlOD1OD2AverageCorrected
    202.076 1.988 2.032 1.887
    101.292 1.196 1.244 1.099
    50.804 0.820 0.812 0.667
    2.50.511 0.521 0.516 0.371
    1.250.324 0.335 0.330 0.185
    0.6250.282 0.278 0.280 0.135
    0.3120.223 0.212 0.218 0.073
    00.146 0.144 0.145 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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