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  4. Rat tight junction protein 1 (ZO1) ELISA Kit

Rat tight junction protein 1 (ZO1) ELISA Kit

  • 中文名稱：
    大鼠緊密連接蛋白1(ZO1)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E17287r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠緊密連接蛋白1(ZO1)酶聯(lián)免疫試劑盒（CSB-E17287r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的ZO1含量。大鼠緊密連接蛋白1（ZO-1）是緊密連接復(fù)合體的重要組成部分，參與維持上皮細(xì)胞間連接的穩(wěn)定性和腸道屏障功能。它通過(guò)連接膜蛋白與細(xì)胞骨架，調(diào)節(jié)細(xì)胞間通透性，對(duì)防止病原體和毒素進(jìn)入體內(nèi)至關(guān)重要。研究顯示，ZO-1的表達(dá)與消化系統(tǒng)疾病的發(fā)生、發(fā)展及預(yù)后密切相關(guān)，其功能異常可能導(dǎo)致腸黏膜屏障破壞和多種消化系統(tǒng)疾病。試劑盒檢測(cè)范圍為31.25 pg/mL-2000 pg/mL，適用于體外研究ZO1在細(xì)胞模型或動(dòng)物模型中的表達(dá)調(diào)控，例如藥物干預(yù)、氧化應(yīng)激或病原體感染等實(shí)驗(yàn)條件下緊密連接功能的動(dòng)態(tài)評(píng)估，為屏障相關(guān)疾病的分子機(jī)制研究提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    DKFZp686M05161, MGC133289, ZO-1, tight junction protein 1|tight junction protein ZO-1|zona occludens 1|zonula occludens 1 protein
  • 縮寫(xiě)：
    ZO1
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測(cè)范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    7.8 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Tags & Cell Markers
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat ZO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 91  
    Range % 86-95  
    1:2 Average % 102  
    Range % 97-107  
    1:4 Average % 91  
    Range % 85-97  
    1:8 Average % 97  
    Range % 91-103  
  • 回收率：
    The recovery of rat ZO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-98  
    EDTA plasma (n=4) 97 90-100  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    2000 2.272 2.374 2.323 2.221  
    1000 1.846 1.967 1.907 1.805  
    500 1.496 1.392 1.444 1.342  
    250 0.962 0.946 0.954 0.852  
    125 0.578 0.596 0.587 0.485  
    62.5 0.364 0.351 0.358 0.256  
    31.2 0.225 0.230 0.228 0.126  
    0 0.101 0.102 0.102    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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