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  4. Rat tissue inhibitors of metalloproteinase 2,TIMP-2 ELISA Kit

Rat tissue inhibitors of metalloproteinase 2,TIMP-2 ELISA Kit

  • 中文名稱：
    大鼠基質(zhì)金屬蛋白酶抑制因子2(TIMP-2)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E07386r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠基質(zhì)金屬蛋白酶抑制因子2(TIMP-2)酶聯(lián)免疫試劑盒（CSB-E07386r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TIMP2含量。TIMP2 即金屬蛋白酶組織抑制因子 2，在細(xì)胞外基質(zhì)代謝、細(xì)胞增殖等生理過程起重要作用。研究發(fā)現(xiàn)其可抑制基質(zhì)金屬蛋白酶活性，在腫瘤等疾病中表達(dá)異常。它參與腫瘤侵襲、轉(zhuǎn)移等機(jī)制，有望成為相關(guān)疾病診斷、治療的潛在靶點(diǎn)。試劑盒檢測范圍為1.25 ng/mL-80 ng/mL，科研人員可將其應(yīng)用于纖維化疾病模型研究、炎癥反應(yīng)機(jī)制探索或腫瘤微環(huán)境分析等領(lǐng)域，通過檢測TIMP - 2水平變化揭示其在基質(zhì)代謝調(diào)控中的作用，為心血管疾病、肝纖維化等病理機(jī)制研究提供實(shí)驗(yàn)依據(jù)。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Timp2 ELISA Kit; Timp-2 ELISA Kit; Metalloproteinase inhibitor 2 ELISA Kit; Tissue inhibitor of metalloproteinases 2 ELISA Kit; TIMP-2 ELISA Kit
  • 縮寫：
    TIMP2
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    1.25 ng/mL-80 ng/mL
  • 靈敏度：
    0.31 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cancer
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat TIMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 91
    Range % 88-95
    1:2 Average % 95
    Range % 90-100
    1:4 Average % 97
    Range % 94-103
    1:8 Average % 101
    Range % 96-105
  • 回收率：
    The recovery of rat TIMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 90 87-94
    EDTA plasma (n=4) 96 94-101
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    80 2.184 2.189 2.187 2.009
    40 1.486 1.456 1.471 1.293
    20 0.925 0.994 0.960 0.782
    10 0.545 0.588 0.567 0.389
    5 0.431 0.443 0.437 0.259
    2.5 0.308 0.292 0.300 0.122
    1.25 0.251 0.233 0.242 0.064
    0 0.179 0.177 0.178 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

相關(guān)問答

■ 常見問題解答
Q:

Can you please provide the following information about this kit:
a. Capture antibody or capture antigen.
b. Detection antibody (if applicable).
c. Standard – if recombinant protein, please specify sequence (including species source and accession no. if available) and expression host.
4) If you have data on the normal value and the value in case of renal failure of TIMP-2 in rats with the kit, please send me too.
5) Please send the current manual.

A:
Thanks for your inquiry.
CSB-E07386r
This kit is designed against full length protein. Pls refer to: https://www.uniprot.org/uniprot/P30121.
Capture antibody is mouse monoclonal antibody and detection antibody is goat polyclonal antibody.
Standard is NS0-derived recombinant protein.
We tested undiluted serum and plasma samples before and the test value is 2.5--10ng/ml.
I will send you the manual by email.

靶點(diǎn)詳情

  • 功能：
    Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor.
  • 基因功能參考文獻(xiàn)：
    1. Dynamics of distribution of capillaries with MMP2 and TIMP2 expressed in rat brain during development of experimental hypertension has been reported. PMID: 29308554
    2. During the processes of conjunctival filtering bleb and scar formation in rats, the changes in MMP-2 and TIMP-2 levels in the filtering area, together with a corresponding proliferation of fibroblasts and the accumulation of collagen fibres, resulted in scarring of filtering blebs. PMID: 29075637
    3. These findings suggested that artesunate attenuates alveolitis and pulmonary fibrosis by regulating expression of collagen-IV, TIMP-1 and 2, as well as MMP-2 and -9, to reduce ECM accumulation. PMID: 27323108
    4. Data suggest that amlodipine and Atorvastatin could improve ventricular remodeling in SHR rats through intervention with the imbalance of MMP-2/TIMP-2 and MMP-9/TIMP-1 system. PMID: 27322513
    5. decreased in diabetic skin PMID: 26047379
    6. Lung tissue from burn animals showed increased matrix metalloproteinase activity, decreased levels of TIMP-2, and no difference in levels of active MMP-9 in burn vs. control groups. PMID: 26601648
    7. MMP-2 and MMP-9 activities and TIMP-1 and TIMP-2 expression were significantly reduced in the lateral prostatic lobe of the ethanol drinking animals. PMID: 26258010
    8. The present study suggests that EPO can exert a cardioprotective effect on DOX-induced cardiotoxicity which may be associated with improving MMP-2/TIMP-2 imbalance. PMID: 24685074
    9. Enhancement of TIMP-2 expression may be a potential therapeutic target for neuroinflammatory disorders. PMID: 24970341
    10. Bone marrow mononuclear cell transplantation significantly increased MMP-9 and MMP-13, and decreased TIMP expression. PMID: 23886643
    11. the expression of TIMP-2 increased only in the neutrophils, endotheliocytes, and fibroblasts, remaining to be equal over all stages. PMID: 23383440
    12. Membrane type 1 MMP, MMP2, and TIMP2 were present in testis during gonocyte migration and transformation into spermatogenic stem cells. PMID: 22974613
    13. Inulicin inhibits hyperplasia of neointima by modulating the balance of MMP-2 and TIMP-2. PMID: 21141515
    14. Data show that MMPs 2 and 9 and their inhibitors, TIMPs 1 and 2, are upregulated in response to oxidative stress produced by HIV-1 gp120 in a rat model of HIV encephalopathy. PMID: 22092673
    15. NDRG2 contributed to an increase in the ratio of matrix metalloproteinase 2 (MMP2) to tissue inhibitor of matrix metalloproteinase 2 PMID: 22110735
    16. Trained spontaneously hypertensive rats showed higher metalloproteinase-2/tissue inhibitor metalloproteinase-2 (MMP-2/TIMP-2) ratio and lower levels of alpha-smooth muscle actin. PMID: 22045123
    17. A dramatic increase of TIMP1 mRNA and a decrease of TIMP2 in DRG after sciatic nerve transection. PMID: 21782897
    18. the present study showed for the first time the mode of TIMP-1 and -2 expression and activity in a rat liver fibrosis model. PMID: 20226641
    19. After transfection with FRNK, MMP-2 was up-regulated and TIMP-2 was down-regulated in hepatic stellate cells. PMID: 18983773
    20. The TIMP-2 gene mediated by adenovirus can renew the balance of degradation of the extracellular matrix caused by elastase and inhibit the formation of an abdominal aortic aneurysm. PMID: 20103929
    21. Addition of follicle stimulating hormone to Sertoli cells increased the TIMP-2 mRNA level three-fold, implicating a role in spermatogenesis. PMID: 11928819
    22. Data show that matrix metalloproteinase-2 and TIMP-2 are likely involved in the FSH-induced changes in Sertoli cells. PMID: 12039062
    23. tissue inhibitor of metalloproteinase-2 mRNA was observed surrounding the developing corpus luteum and was present in granulosa-lutein cells, with high levels near the center of the corpus luteum PMID: 12444073
    24. results suggest that the migration and invasion of cardiac fibroblasts is furin-dependent and that the active species of MT1-MMP and MMP-2 may be involved in both events PMID: 12970340
    25. In the process of hepatic fibrosis, fibroblasts and myofibroblasts are the major cells that express TIMPs and the more serious the hepatic fibrosis is in the injured liver, the higher the level of TIMP-1 and TIMP-2 gene expression. PMID: 14695775
    26. Blood leukocytes and loss of flow are major factors in the activation of MMP-2, and cytokine-mediated differential regulation of TIMP-1 and TIMP-2 may contribute significantly to blood brain barrier failure. PMID: 16707552
    27. MMP2 plays a role in the development of BC metastases. Furthermore, our results suggest that astrocyte factors and the ERK1/2 signaling pathway may be associated with BC brain metastasis development. PMID: 17505812
    28. matrix metalloproteinase-2, tissue inhibitor of metalloproteinase-1 and osteopontin have roles in heart failure in DOCA-salt hypertensive rat PMID: 18415800
    29. TIMP-2 is an osteoanabolic factor in vitro and in vivo PMID: 18558556
    30. The induction of MMP-2, MMP-9, TIMP-1 and TIMP-2 mRNAs post-castration could be at least in part due to post-transcriptional stabilization. PMID: 18675881
    31. investigated the involvement of matrix metalloproteinases (MMPs), tissue inhibitor (TIMP) and endothelin-1 (ET-1) in the renal damage in spontaneously hypertensive rats following nitric oxide deprivation. PMID: 18935914
    32. over-expressed ZAK may contribute to the progression of myocardial remodeling by disrupting the balance in the MMP-2/9 and TIMP-1/2 expression level, respectively PMID: 19184368

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Protease inhibitor I35 (TIMP) family
  • 數(shù)據(jù)庫鏈接：