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  4. Rat tumor necrosis factor-inducible gene 6 protein(TSG-6) elisa kit

Rat tumor necrosis factor-inducible gene 6 protein(TSG-6) elisa kit

  • 中文名稱：
    大鼠腫瘤壞死因子誘導(dǎo)蛋白6（TSG-6）酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E17373r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠腫瘤壞死因子誘導(dǎo)蛋白6（TSG-6）酶聯(lián)免疫試劑盒（CSB-E17373r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TSG-6含量。大鼠腫瘤壞死因子誘導(dǎo)蛋白6（TSG-6）是一種分泌蛋白，具有透明質(zhì)酸結(jié)合域，參與細(xì)胞外基質(zhì)穩(wěn)定和細(xì)胞遷移。它通過增強(qiáng)α-間抑制劑（IαI）的活性，在炎癥相關(guān)的蛋白酶網(wǎng)絡(luò)中發(fā)揮重要作用。TSG-6的表達(dá)受TNF-α和IL-1等信號分子誘導(dǎo)，并與蛋白多糖合成和聚集有關(guān)。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于基礎(chǔ)科研中探索炎癥性疾病機(jī)制、評估組織損傷修復(fù)模型或腫瘤相關(guān)研究中的TSG - 6動態(tài)變化本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    TSG-6, TSG6, hyaluronate-binding protein|tumor necrosis factor alpha-inducible protein 6|tumor necrosis factor-inducible protein 6|tumor necrosis factor-stimulated gene-6 protein
  • 縮寫：
    TSG-6
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.078 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cancer
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat TSG-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 91  
    Range % 87-95  
    1:2 Average % 101  
    Range % 97-104  
    1:4 Average % 91  
    Range % 87-94  
    1:8 Average % 100  
    Range % 96-104  
  • 回收率：
    The recovery of rat TSG-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 91-98  
    EDTA plasma (n=4) 94 91-97  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    20 2.545 2.532 2.539 2.373  
    10 1.903 1.867 1.885 1.719  
    5 1.210 1.222 1.216 1.050  
    2.5 0.683 0.718 0.701 0.535  
    1.25 0.429 0.448 0.439 0.273  
    0.625 0.341 0.332 0.337 0.171  
    0.312 0.256 0.243 0.250 0.084  
    0 0.168 0.164 0.166 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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