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  4. Rat urokinase plasminogen activator,uPA ELISA kit

Rat urokinase plasminogen activator,uPA ELISA kit

  • 中文名稱：
    大鼠尿激酶型纖溶酶原激活物(uPA)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E07368r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠尿激酶型纖溶酶原激活物(uPA)酶聯(lián)免疫試劑盒（CSB-E07368r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PLAU含量。PLAU是一種重要的靶點。它參與多種生理病理過程，與腫瘤細胞的侵襲、轉(zhuǎn)移密切相關(guān)。在研究機制方面，主要聚焦于其酶活性及信號通路，明確它對腫瘤微環(huán)境的調(diào)控作用，為開發(fā)針對性的抗腫瘤藥物指明方向。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于心血管疾病、纖維化疾病或腫瘤相關(guān)動物模型中纖溶系統(tǒng)活性的動態(tài)監(jiān)測，以及細胞遷移、組織修復等基礎(chǔ)研究中的uPA表達水平分析，為科研人員探究uPA在病理生理過程中的調(diào)控機制提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    PlauUrokinase-type plasminogen activator ELISA kit; U-plasminogen activator ELISA kit; uPA ELISA kit; EC 3.4.21.73) [Cleaved into: Urokinase-type plasminogen activator long chain A; Urokinase-type plasminogen activator short chain A; Urokinase-type plasminogen activator chain B] ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat uPA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 91    
    Range % 88-95    
    1:2 Average % 95    
    Range % 90-100    
    1:4 Average % 97    
    Range % 94-103    
    1:8 Average % 101    
    Range % 96-105    
  • 回收率：
    The recovery of rat uPA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 90 87-94    
    EDTA plasma (n=4) 96 94-101    
                   
                   
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    1000 2.334 2.186 2.260 2.077    
    500 1.652 1.625 1.639 1.456    
    250 1.047 0.997 1.022 0.839    
    125 0.636 0.688 0.662 0.479    
    62.5 0.398 0.402 0.400 0.217    
    31.2 0.325 0.337 0.331 0.148    
    15.6 0.246 0.232 0.239 0.056    
    0 0.189 0.177 0.183 　    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Peptidase S1 family
  • 數(shù)據(jù)庫鏈接：