Rat visfatin ELISA Kit
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中文名稱:大鼠內(nèi)脂素/內(nèi)臟脂肪素(visfatin)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-E08941r
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規(guī)格:96T/48T
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價(jià)格:¥3900/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:大鼠內(nèi)脂素/內(nèi)臟脂肪素(visfatin)酶聯(lián)免疫試劑盒(CSB-E08941r)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的NAMPT含量。NAMPT(尼克酰胺磷酸核糖轉(zhuǎn)移酶)是哺乳動(dòng)物細(xì)胞中煙酰胺腺嘌呤二核苷酸(NAD)補(bǔ)救合成途徑的關(guān)鍵酶,參與能量代謝、抗氧化反應(yīng)等。研究發(fā)現(xiàn),NAMPT與腫瘤發(fā)生發(fā)展密切相關(guān),抑制NAMPT可誘導(dǎo)細(xì)胞凋亡、自噬,抑制細(xì)胞增殖和血管生成,具有抗腫瘤作用。試劑盒檢測(cè)范圍為3.12 ng/mL-200 ng/mL,適用于體外實(shí)驗(yàn)中評(píng)估大鼠模型或離體樣本的內(nèi)脂素表達(dá)水平,可應(yīng)用于肥胖相關(guān)代謝機(jī)制研究、脂肪組織功能分析、藥物干預(yù)效果評(píng)價(jià)等科研領(lǐng)域。其兼容多種生物樣本的特性支持研究者同時(shí)分析循環(huán)系統(tǒng)(血清/血漿)與局部組織(如脂肪、肝臟等)的內(nèi)脂素分布特征,為探索其在能量代謝、炎癥調(diào)控等生理病理過(guò)程中的作用提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
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別名:Nampt ELISA Kit; Pbef1 ELISA Kit; Nicotinamide phosphoribosyltransferase ELISA Kit; NAmPRTase ELISA Kit; Nampt ELISA Kit; EC 2.4.2.12 ELISA Kit; Pre-B-cell colony-enhancing factor 1 homolog ELISA Kit; PBEF ELISA Kit; Visfatin ELISA Kit
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縮寫(xiě):
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Uniprot No.:
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種屬:Rattus norvegicus (Rat)
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樣本類型:serum, plasma, tissue homogenates
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檢測(cè)范圍:3.12 ng/mL-200 ng/mL
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靈敏度:0.78 ng/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Metabolism
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat visfatin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 87 Range % 82-97 1:2 Average % 96 Range % 92-103 1:4 Average % 102 Range % 96-108 1:8 Average % 94 Range % 89-98 -
回收率:
The recovery of rat visfatin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 90-100 EDTA plasma (n=4) 98 94-108 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 200 1.743 1.867 1.805 1.707 100 1.304 1.199 1.252 1.154 50 0.835 0.838 0.837 0.739 25 0.534 0.547 0.541 0.443 12.5 0.348 0.336 0.342 0.244 6.25 0.277 0.268 0.273 0.175 3.12 0.171 0.177 0.174 0.076 0 0.098 0.097 0.098 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Irradiation of carotid baroreceptor with low‐intensity pulsed ultrasound exerts different metabolic protection in perirenal, epididymal white adipose tissue and interscapular brown adipose tissue of obese rats Q Cao,FASEB J,2020
- An optimized dose of raspberry ketones controls hyperlipidemia and insulin resistance in male obese rats: Effect on adipose tissue expression of adipocytokines and Aquaporin 7 Eman T.Mehanna.et al,European Journal of Pharmacology,2018
- Effect of glucagon-like peptide-1 analogue; Exendin-4, on cognitive functions in type 2 diabetes mellitus; possible modulation of brain derived neurotrophic factor Abdelwahed OM.et al,Brain Res Bull,2018
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能:Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-ARNTL/BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression.
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基因功能參考文獻(xiàn):
- A Strong association was observed among fasting blood glucose, serum visfatin and trace elements levels of high fat diet and type 2 diabetes mellitus PMID: 29772226
- Our findings suggest that NAMPT inhibition may be a novel therapeutic approach for I/R-induced lung injury. The protective effects involve the suppression of multiple signal pathways. PMID: 28438162
- Thus, it can be suggested that visfatin may play an important role in testicular aging by regulating testicular steroidogenesis and spermatogenesis. PMID: 28163205
- visfatin plays a dual role in the apoptosis in rat Mesenteric lymph nodes, which is mediated by both the mitochondrial apoptotic pathway and the death-receptor apoptotic pathway. PMID: 26857566
- this study shows that serum visfatin levels, and white adipose tissue and hepatic visfatin mRNA levels of both male and female rats were increased by LPS injection PMID: 27083000
- Visfatin was expressed in the rats' retinas, mainly in the retinal nerve fibre layer, inner plexiform layer and outer plexiform layer. As the development of diabetes mellitus course, its expression was gradually increased. PMID: 26694625
- NAMPT protects against 6-hydroxydopamine-induced neurotoxicity in PC12 cells through modulating SIRT1 activity PMID: 27035562
- Report retinal toxicity of NAMPT inhibitors. PMID: 25505128
- visfatin could participate in the inflammatory process in rat spleen by modulating the macrophages and inflammatory cytokines. PMID: 25358398
- Visfatin induced the expression of alphaSMA in cultureactivated rat HSC, which was accompanied by a significant increase in collagen types I and III. PMID: 25351242
- These data are the first to show that Nampt inhibits myometrial contractility and does so more potently than leptin. PMID: 25645057
- Central visfatin improved glucose homeostasis by increasing insulin secretion and insulin sensitivity at euglycemia through the hypothalamus in diabetic rats. PMID: 24332931
- role in myocardial inflammation during endotoxemia PMID: 23831038
- Data indicate that nicotinamide phosphoribosyltransferase (NAMPT) mRNA and protein were increased in the kidneys with streptozotocin-induced diabetes. PMID: 24287278
- CR-induced beneficial effects in oxidative stress, mitochondrial biogenesis, and metabolic adaptation require NAMPT. PMID: 23946338
- visfatin plays a critical role in cardioprotection by suppressing myocardial apoptosis via AMPK activation PMID: 23065734
- Nampt promotes neuronal survival through inducing autophagy. PMID: 22113203
- Visfatin expression was increased mainly through the AT1-R-JAK/STAT pathway in the process of Ang II-induced cardiomyocyte hypertrophy. PMID: 21841463
- The expression of visfatin is closely associated with the expression of proinflammatory cytokines in FFA-induced inflammation and is significantly decreased by NF-kappaB inhibition in HepG2 cells. PMID: 21664630
- Our findings reveal that Nampt protects against ischemic stroke through rescuing neurons from death via the SIRT1-dependent AMPK pathway and indicate that Nampt is a new therapeutic target for stroke. PMID: 21246601
- results of the present study demonstrate that serum visfatin levels in Lyon hypertensive rats are elevated and are associated with lipid metabolic abnormalities PMID: 20456420
- No differences of serum visfatin levels were observed between spontaneously hypertensive rats and Wistar-Kyoto rats. Serum visfatin levels were significantly correlated to weights of skeletal muscle. PMID: 20658215
- Visfatin exhibits important autocrine effects on sensitivity of liver cells to insulin action possibly through its effects on NAD biosynthesis. PMID: 19929131
- Exercise training induced SIRT1 activity due to the positive effect of exercise on the activity of nicotinamide phosphoribosyltransferase (NAMPT) and thereby the production of sirtuin-fueling NAD(+). PMID: 19913571
- PBEF is not a cytokine-like secreted protein but an intracellular protein associated with the cell cycle PMID: 12782293
- relative visfatin gene expression is not associated with the metabolic syndrome in WOKW rats [Pbef, also called visfatin] PMID: 15922301
- Results describe the crystal structure of visfatin in three different forms: apo and in complex with either nicotinamide mononucleotide (NMN) or the nicotinamide phosphoribosyltransferase inhibitor FK-866. PMID: 16901503
- During fasting, increased Nampt provides protection against cell death and requires an intact mitochondrial NAD(+) salvage pathway as well as the mitochondrial NAD(+)-dependent deacetylases SIRT3 and SIRT4. PMID: 17889652
- Visfatin is produced in mesangial cells, which are a novel target for visfatin, and play an important role in the pathogenesis of diabetic nephropathy. PMID: 18768589
- improves insulin sensitivity and exerts its hypocholesterolemic effects at least partially through upregulation of the tyrosine phosphorylation of IRS-1 protein and the mRNA levels of PPARgamma and SREBP-2 PMID: 19263259
- The crystal structure of rat visfatin in complex with IS001, is desribed. PMID: 19533035
- Nicotinamide phosphoribosyltransferase regulates cell survival through NAD+ synthesis in cardiac myocytes. PMID: 19661458
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亞細(xì)胞定位:Nucleus. Cytoplasm. Secreted.
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蛋白家族:NAPRTase family
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組織特異性:Expressed in various tissues. At the highest level in liver and at the second highest in heart. The amount is higher in heart than in lung.
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