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  4. Rat α-enolase ELISA Kit

Rat α-enolase ELISA Kit

  • 中文名稱：
    大鼠α烯醇化酶(α-enolase)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E17439r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    大鼠α烯醇化酶(α-enolase)酶聯(lián)免疫試劑盒（CSB-E17439r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的ENO1含量。ENO1是一個重要靶點。它參與糖酵解等關鍵代謝過程。研究發(fā)現(xiàn)，ENO1異常表達與腫瘤發(fā)生發(fā)展密切相關，其可能通過影響能量代謝、細胞增殖與凋亡等機制來發(fā)揮作用，正成為腫瘤治療等領域有潛力的研究方向。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，適用于科研場景下對大鼠模型代謝通路異常、疾病機制探索或藥物干預效果評估等實驗需求，為生命科學基礎研究提供可靠工具。 其在代謝性疾病、腫瘤微環(huán)境及炎癥反應等研究領域具有重要價值。本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
  • 別名：
    Eno1 ELISA Kit; Eno-1Alpha-enolase ELISA Kit; EC 4.2.1.11 ELISA Kit; 2-phospho-D-glycerate hydro-lyase ELISA Kit; Enolase 1 ELISA Kit; Non-neural enolase ELISA Kit; NNE ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.625 ng/mL-40 ng/mL
  • 靈敏度：
    0.156 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat α-enolase in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 93
    Range % 88-97
    1:2 Average % 102
    Range % 97-107
    1:4 Average % 101
    Range % 95-105
    1:8 Average % 94
    Range % 91-98
  • 回收率：
    The recovery of rat α-enolase spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 105 99-108
    EDTA plasma (n=4) 87 84-92
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    40 2.191 2.275 2.233 2.111
    20 1.448 1.538 1.493 1.371
    10 0.921 0.963 0.942 0.820
    5 0.563 0.572 0.568 0.446
    2.5 0.334 0.341 0.338 0.216
    1.25 0.231 0.245 0.238 0.116
    0.625 0.173 0.179 0.176 0.054
    0 0.121 0.122 0.122 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Glycolytic enzyme the catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. In addition to glycolysis, involved in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
  • 基因功能參考文獻：
    1. gamma-enolase may signal and correlate lung tissue damage well before obvious physical pathological tissue damage and might be a candidate diagnostic and/or prognostic marker. PMID: 29728894
    2. The present study aimed to investigate the change of alpha-enolase during cardiac hypertrophy and explore its role in this pathological process. PMID: 28910549
    3. alpha-enolase has a catalytically independent role in inducing cardiomyocyte apoptosis and mitochondrial dysfunction. PMID: 25446184
    4. this research suggests that the protective effect of Hsc70 on the cardiomyocytes against oxidative stress is partly associated with its interaction with alpha-enolase. PMID: 21958194
    5. alpha-Enolase binds to RNA PMID: 21621582
    6. The alpha-enolase hyper-phosphorylation in hypertrophied left ventricle was accompanied by decreased enzymatic activity. PMID: 18468517
    7. Data show that the expression of alpha-enolase in the left ventricle of the rats during normal and pathological cardiac development is different. PMID: 19450578
    8. enolase alpha was up-regulated in left ventricle of spontaneously hypertensive rats. PMID: 16485256

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  • 亞細胞定位：
    Cytoplasm. Cell membrane. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
  • 蛋白家族：
    Enolase family
  • 組織特異性：
    Expressed in flagella of epididymal sperm. The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma
  • 數(shù)據(jù)庫鏈接：