Sheep C-reactive protein(CRP) ELISA Kit

產(chǎn)品詳情

說(shuō)明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

綿羊C反應(yīng)蛋白(CRP)酶聯(lián)免疫試劑盒（CSB-E15081Sh）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的CRP含量。CRP（C反應(yīng)蛋白）是一種由肝臟合成的蛋白質(zhì)，當(dāng)體內(nèi)發(fā)生炎癥時(shí)，CRP水平會(huì)升高。它作為炎癥標(biāo)志物，能結(jié)合肺炎鏈球菌等病原體，激活補(bǔ)體系統(tǒng)，增強(qiáng)白細(xì)胞吞噬作用，參與非特異性免疫反應(yīng)。試劑盒檢測(cè)范圍為0.625 μg/mL-40 μg/mL，適用于綿羊模型中炎癥機(jī)制研究、疾病進(jìn)程評(píng)估及免疫調(diào)控相關(guān)科研實(shí)驗(yàn)，例如感染性疾病模型構(gòu)建中CRP動(dòng)態(tài)監(jiān)測(cè)、應(yīng)激反應(yīng)評(píng)估或藥物干預(yù)效果分析本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
縮寫：

CRP
Uniprot No.：

W5PD71
種屬：

Ovis aries (Sheep)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測(cè)范圍：

0.625 μg/mL-40 μg/mL
靈敏度：

0.156 μg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Immunology
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of sheep CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-90
1:2	Average %	96
	Range %	92-100
1:4	Average %	92
	Range %	87-96
1:8	Average %	95
	Range %	87-98

回收率：

The recovery of sheep CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-96
EDTA plasma (n=4)	90	84-95

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.