Sheep Interleukin 12,IL-12 ELISA Kit

產(chǎn)品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

綿羊白介素12(IL-12)酶聯(lián)免疫試劑盒（CSB-E14017Sh）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL12含量。IL12是重要的細胞因子，在免疫調(diào)節(jié)中發(fā)揮關鍵作用。它能促進T細胞和NK細胞的活化和增殖，增強機體免疫應答。研究聚焦其信號傳導機制、與疾病關聯(lián)。深入了解IL12機制，有望為免疫相關疾病如腫瘤、自身免疫病治療帶來新策略。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，本產(chǎn)品適用于綿羊免疫相關疾病的機制研究，如感染性疾病模型中細胞免疫應答強度的動態(tài)監(jiān)測、疫苗效力評估實驗中的Th1型免疫激活分析，或炎癥性疾病研究中的細胞因子網(wǎng)絡調(diào)控解析，為獸醫(yī)科學及比較醫(yī)學研究提供精準的科研工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

IL-12
種屬：

Ovis aries (Sheep)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.81 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of sheep IL-12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	87-94
1:2	Average %	103
	Range %	99-107
1:4	Average %	93
	Range %	87-99
1:8	Average %	91
	Range %	88-96

回收率：

The recovery of sheep IL-12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	92-103
EDTA plasma (n=4)	90	86-94

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.