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  4. Sheep Interleukin 2,IL-2 ELISA Kit

Sheep Interleukin 2,IL-2 ELISA Kit

  • 中文名稱：
    綿羊白介素2(IL-2)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E11217Sh
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    綿羊白介素2(IL-2)酶聯(lián)免疫試劑盒（CSB-E11217Sh）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的IL2含量。IL2是重要的免疫靶點(diǎn)。它是機(jī)體免疫應(yīng)答的關(guān)鍵調(diào)節(jié)因子，可刺激T細(xì)胞和NK細(xì)胞增殖、分化。研究上主要聚焦其在抗腫瘤、自身免疫病等方面的機(jī)制，通過(guò)調(diào)節(jié)IL2信號(hào)通路有望研發(fā)新療法來(lái)增強(qiáng)免疫或抑制過(guò)度免疫。試劑盒檢測(cè)范圍為31.25 pg/mL-2000 pg/mL，該產(chǎn)品適用于動(dòng)物免疫學(xué)研究、疫苗效力評(píng)估、炎癥反應(yīng)模型構(gòu)建等科研場(chǎng)景，為探究綿羊免疫系統(tǒng)功能、感染性疾病機(jī)制或藥物干預(yù)效果提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    IL2Interleukin-2 ELISA kit; IL-2 ELISA kit; T-cell growth factor ELISA kit; TCGF ELISA kit
  • 縮寫(xiě)：
  • Uniprot No.：
  • 種屬：
    Ovis aries (Sheep)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    7.81 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of sheep IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %89
    Range %84-93
    1:2Average %98
    Range %91-105
    1:4Average %97
    Range %95-103
    1:8Average %92
    Range %86-98
  • 回收率：
    The recovery of sheep IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-99
    EDTA plasma (n=4)9690-100
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.418 2.425 2.422 2.335
    10001.547 1.556 1.552 1.465
    5001.085 1.063 1.074 0.987
    2500.677 0.658 0.668 0.581
    1250.436 0.407 0.422 0.335
    62.50.240 0.234 0.237 0.150
    31.250.162 0.159 0.161 0.074
    00.088 0.086 0.087 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    IL-2 family
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: oas:443401

    UniGene: Oar.436