Sheep Interleukin 4(IL-4)ELISA Kit

產品詳情

文獻引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

綿羊白介素4(IL-4)酶聯(lián)免疫試劑盒（CSB-E15963Sh）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL4含量。IL4是一種由活化的T淋巴細胞、肥大細胞、嗜堿性粒細胞產生的多效性細胞因子，在免疫調節(jié)中起關鍵作用。它通過誘導B細胞分化、促進抗體產生和調節(jié)Th2細胞介導的免疫反應，在過敏性疾病和腫瘤免疫中扮演重要角色。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，適用于免疫學研究、炎癥性疾病模型分析、疫苗免疫效果評估等科研領域，可用于探究綿羊感染性疾病、寄生蟲免疫應答及自身免疫機制，為其提供精準的蛋白定量數(shù)據(jù)。本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
別名：

IL4 ELISA Kit; IL-4Interleukin-4 ELISA Kit; IL-4 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BSF-1 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit
縮寫：

IL4
Uniprot No.：

P30368
種屬：

Ovis aries (Sheep)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

6.25 pg/mL-400 pg/mL
靈敏度：

1.56 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of sheep IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-95
1:2	Average %	102
	Range %	97-105
1:4	Average %	95
	Range %	90-97
1:8	Average %	97
	Range %	93-100

回收率：

The recovery of sheep IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-97
EDTA plasma (n=4)	96	90-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
基因功能參考文獻：
1. Local production of the prototypic immunomodulatory cytokine IL-4 in transplanted corneal endothelium induces eosinophilia, inflammation, and rejection. PMID: 16081789
2. Early IL-4 gene expression in abomasum is associated with resistance to Haemonchus infection. PMID: 27059919
亞細胞定位：

Secreted.
蛋白家族：

IL-4/IL-13 family
數(shù)據(jù)庫鏈接：

KEGG: oas:443327

UniGene: Oar.377