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Sheep Somatotropin(GH1) ELISA kit

  • 中文名稱：
    綿羊生長激素(GH1)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL009407SH
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    綿羊生長激素(GH1)酶聯(lián)免疫試劑盒（CSB-EL009407SH）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的GH1含量。GH1 是生長激素 1 基因，其編碼生長激素。它在調(diào)節(jié)身體生長、細(xì)胞增殖與再生等方面扮演重要角色。研究機制主要聚焦于其信號通路，探究它如何與受體結(jié)合、激活下游信號，以調(diào)控生長發(fā)育相關(guān)基因表達(dá)，助力疾病治療研究。試劑盒檢測范圍為2.5 ng/mL-40 ng/mL，適用于科研領(lǐng)域探究生長激素在綿羊生理調(diào)控中的作用機制。本產(chǎn)品可支持畜牧科研中生長性能評估、營養(yǎng)代謝實驗、繁殖周期監(jiān)測等研究場景，尤其適用于分析不同飼養(yǎng)條件或激素干預(yù)下GH1的動態(tài)變化。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    GH1 ELISA Kit; Somatotropin ELISA Kit; Growth hormone ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Ovis aries (Sheep)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    2.5 ng/mL-40 ng/mL
  • 靈敏度：
    0.675 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of sheep GH1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 90-101  
    1:2 Average % 93  
    Range % 87-98  
    1:4 Average % 96  
    Range % 95-102  
    1:8 Average % 95  
    Range % 89-99  
  • 回收率：
    The recovery of sheep GH1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 90-102  
    EDTA plasma (n=4) 92 88-96  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    40 0.173 0.175 0.174    
    20 0.308 0.312 0.310    
    10 0.639 0.644 0.642    
    5 1.401 1.408 1.405    
    2.5 2.261 2.265 2.263    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
  • 基因功能參考文獻(xiàn)：
    1. Association analyses showed that both g.498G>C and g.616G>A GH polymorphisms were significantly associated with several growth traits (at P < 0.01 or P < 0.05) in three investigated sheep breeds. PMID: 27706626
    2. The effect leptin, orexin-A, and ghrelin on the secretion of luteinizing hormone (LH) and growth hormone by adenohypophyseal explants obtained from normally fed or fasted ewes during the breeding and nonbreeding seasons. PMID: 24906931
    3. The results showed that the growth hormone genotypes are associated with estimated breeding values of growth traits. PMID: 21328104
    4. The simultaneous increase of expression of hypothalamic NPY suggests NPY involvement in the regulation of psychoemotional stress through the somatotrophic axis in the female lambs. PMID: 20675289
    5. These data are consistent with the effects of LPS on GH secretion originating through peripheral cytokine presentation to the pituitary, as well as a potential to act directly on selective populations of pituitary cells via CD14. PMID: 15899940
    6. Results show that in comparison to the product of the ovine growth hormone (GH)2-N gene copy, the postulated product of the GH2-Z gene copy, the G9R/G63S mutein, has a higher affinity to the growth hormone receptor and elevated biological activity. PMID: 17850798
    7. Results describe thet pulsatility of cerebrospinal fluid and plasma ghrelin levels in relation to growth hormone secretion and food intake in the sheep, and the effects of exogenous ghrelin on GH and feeding behavior. PMID: 18673413
    8. study has demonstrated for the first time that ghrelin differentially modulates growth hormone secretory response to growth hormone-releasing hormone according to feeding states in ruminant animals PMID: 19342191

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Somatotropin/prolactin family
  • 數(shù)據(jù)庫鏈接：

    KEGG: oas:443329

    UniGene: Oar.641