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  4. Sheep Tumor Necrosis Factor α,TNF-α ELISA Kit

Sheep Tumor Necrosis Factor α,TNF-α ELISA Kit

  • 中文名稱：
    綿羊腫瘤壞死因子α(TNF-α)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E13853Sh
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    綿羊腫瘤壞死因子α(TNF-α)酶聯(lián)免疫試劑盒（CSB-E13853Sh）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的TNF含量。TNF（腫瘤壞死因子）是一種多功能促炎細(xì)胞因子，最初因能引起腫瘤組織壞死而得名。其超家族包括19個(gè)配體和29個(gè)受體，參與宿主防御、炎癥、細(xì)胞凋亡等生理過(guò)程。TNF通過(guò)與其受體結(jié)合，激活下游信號(hào)通路，調(diào)節(jié)細(xì)胞存活、凋亡和炎癥反應(yīng)。試劑盒檢測(cè)范圍為3.125 pg/mL-50 pg/mL，TNF-α的動(dòng)態(tài)監(jiān)測(cè)對(duì)綿羊感染性疾病模型、疫苗研發(fā)及免疫機(jī)制研究具有重要價(jià)值；可高效支持動(dòng)物醫(yī)學(xué)研究中炎癥相關(guān)疾病模型的建立、病原體感染后免疫應(yīng)答評(píng)估以及抗炎藥物作用機(jī)制的體外實(shí)驗(yàn)，為畜牧科研中的免疫調(diào)控研究提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    TNF ELISA Kit; TNFA ELISA Kit; TNFSF2 ELISA Kit; Tumor necrosis factor ELISA Kit; Cachectin ELISA Kit; TNF-alpha ELISA Kit; Tumor necrosis factor ligand superfamily member 2 ELISA Kit; TNF-a) [Cleaved into: Tumor necrosis factor ELISA Kit; membrane form ELISA Kit; N-terminal fragment ELISA Kit; NTF); Intracellular domain 1 ELISA Kit; ICD1); Intracellular domain 2 ELISA Kit; ICD2); C-domain 1; C-domain 2; Tumor necrosis factor ELISA Kit; soluble form] ELISA Kit
  • 縮寫(xiě)：
  • Uniprot No.：
  • 種屬：
    Ovis aries (Sheep)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    3.125 pg/mL-50 pg/mL
  • 靈敏度：
    3.125 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of sheep TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 84  
    Range % 80-88  
    1:2 Average % 95  
    Range % 90-99  
    1:4 Average % 90  
    Range % 85-94  
    1:8 Average % 102  
    Range % 98-109  
  • 回收率：
    The recovery of sheep TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 89 84-95  
    EDTA plasma (n=4) 96 90-101  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    50 0.161 0.165 0.163    
    25 0.308 0.324 0.316    
    12.5 0.551 0.512 0.532    
    6.25 1.012 0.982 0.997    
    3.125 2.054 2.011 2.033    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
  • 基因功能參考文獻(xiàn)：
    1. The aim of the study was to assess the prognostic significance of Tumor Necrosis Factor-alpha, Interleukin-12(p40), Interleukin-6 and Interleukin 10 levels in cerebrospinal fluid (CSF) in sheep with encephalitic listeriosis. PMID: 25817420
    2. The p38 MAPK inhibitor decreased (P < 0.01) gene expression of TNFa but its effect was not observed at the level of TNFa protein synthesis PMID: 24995301
    3. TNF-alpha gene expression in multifidus was greater on both sides in the disc lesion animals than in the muscle of control animals. PMID: 24718080
    4. Injection of peptidoglycan-polysaccharide resulted in fever, increased concentrations of TNF-alpha on the day of and the day after challenge, and decreased concentrations of progesterone on days 14 and 21. PMID: 20603027
    5. These data are consistent with the effects of LPS on GH secretion originating through peripheral cytokine presentation to the pituitary, as well as a potential to act directly on selective populations of pituitary cells via CD14. PMID: 15899940
    6. plasma IL-1 beta, TNF-alpha and MCP-1 may potentially serve as markers to monitor prognosis of pregnancy toxaemia in ewes PMID: 17221363

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  • 亞細(xì)胞定位：
    Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, membrane form]: Membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, soluble form]: Secreted.; [C-domain 1]: Secreted.; [C-domain 2]: Secreted.
  • 蛋白家族：
    Tumor necrosis factor family
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: oas:443540

    UniGene: Oar.455