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Sheep arachidonic Acid(AA) ELISA kit

  • 中文名稱:
    綿羊花生四烯酸(AA)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-EQ027590SH
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥4200/¥3000
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    綿羊花生四烯酸(AA)酶聯(lián)免疫試劑盒(CSB-EQ027590SH)為競爭法ELISA試劑盒,定量檢測血清、血漿樣本中的AA含量。試劑盒檢測范圍為15.625 ng/mL-1000 ng/mL,靈敏度為13.797 ng/mL。該產(chǎn)品適用于科研領(lǐng)域中對綿羊模型炎癥機(jī)制、營養(yǎng)代謝調(diào)控、藥物干預(yù)效果的評(píng)價(jià),以及脂質(zhì)代謝相關(guān)通路的基礎(chǔ)研究,為體外實(shí)驗(yàn)提供可靠的定量數(shù)據(jù)支持本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫:
    AA
  • 種屬:
    Ovis aries (Sheep)
  • 樣本類型:
    serum, plasma
  • 檢測范圍:
    15.625 ng/mL-1000 ng/mL
  • 靈敏度:
    13.797 ng/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Biochemicals
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of sheep AA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
      Sample Serum(n=4)    
    1:100 Average % 96    
    Range % 89-100    
    1:200 Average % 90    
    Range % 85-95    
    1:400 Average % 100    
    Range % 94-104    
    1:800 Average % 105    
    Range % 97-110    
  • 回收率:
    The recovery of sheep AA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 102 95-106    
    EDTA plasma (n=4) 102 96-107    
                   
                   
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average      
    1000 0.122 0.120 0.121      
    500 0.166 0.158 0.162      
    250 0.232 0.241 0.237      
    125 0.337 0.344 0.341      
    62.5 0.528 0.510 0.519      
    31.25 0.941 0.955 0.948      
    15.625 1.471 1.522 1.497      
    0 2.326 2.419 2.373      
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 最新研究進(jìn)展:
        AA是一種抗氧化劑,最新研究發(fā)現(xiàn),AA對于心血管疾病的預(yù)防有益。一項(xiàng)針對高危心血管患者的研究表明,AA的補(bǔ)充可以降低冠心病發(fā)生率和死亡率。