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ABCB1 Monoclonal Antibody

  • 中文名稱：
    ABCB1鼠單克隆抗體
  • 貨號：
    CSB-MA001046A0m
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: HepG2 whole cell lysate, 293 whole cell lysate, Hela whole cell lysate, SY5Y whole cell lysate, U251 whole cell lysate
      All lanes: ABCB1 antibody at 1:1000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 141 kDa
      Observed band size: 141-250 KDa
      Exposure time：5min
    • Immunofluorescence staining of HepG2 cells with CSB-MA001046A0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
    • Immunofluorescence staining of Hela cells with CSB-MA001046A0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
    • Overlay Peak curve showing Hela cells stained with CSB-MA001046A0m (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    ABCB1單克隆抗體（CUSABIO貨號：CSB-MA001046A0m）是針對人源ABCB1/P-糖蛋白研發(fā)的小鼠源IgG1 Kappa型單克隆抗體，適用于ELISA、Western Blot、免疫熒光（IF）及流式細(xì)胞術(shù)（FC）等多種實驗場景。ABCB1基因編碼的P-糖蛋白屬于ATP結(jié)合盒（ABC）轉(zhuǎn)運蛋白超家族成員，廣泛分布于腫瘤細(xì)胞膜及血腦屏障內(nèi)皮細(xì)胞，其過表達(dá)可通過外排化療藥物介導(dǎo)腫瘤多藥耐藥性，是癌癥治療及藥物代謝研究的關(guān)鍵靶點。本產(chǎn)品采用重組人源ABCB1蛋白片段（NP_000918, 620-709氨基酸區(qū)域）作為免疫原，經(jīng)雜交瘤技術(shù)制備而成，具有高特異性識別能力，保存條件為-20°C以下分裝避光儲存。該抗體可助力于腫瘤耐藥機制解析、藥物轉(zhuǎn)運蛋白功能研究及血腦屏障調(diào)控等基礎(chǔ)科研領(lǐng)域，為探索ABCB1在細(xì)胞內(nèi)外源性物質(zhì)代謝中的分子機制提供可靠工具。
  • 產(chǎn)品名稱：
    Mouse anti-Homo sapiens (Human) ABCB1 Monoclonal antibody
  • Uniprot No.：
  • 基因名：
  • 別名：
    ABC20 antibody; ABCB1 antibody; ATP binding cassette, sub family B (MDR/TAP), member 1 antibody; ATP-binding cassette sub-family B member 1 antibody; CD243 antibody; CLCS antibody; Colchicin sensitivity antibody; Doxorubicin resistance antibody; GP170 antibody; MDR1 antibody; MDR1_HUMAN antibody; Multidrug resistance 1 antibody; Multidrug resistance protein 1 antibody; P glycoprotein 1 antibody; P gp antibody; P-glycoprotein 1 antibody; PGY1 antibody
  • 宿主：
    Mouse
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    Recombinant Human ATP-dependent translocase ABCB1 protein (350-710AA)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    IgG2b
  • 純化方式：
    >95%, Protein A purified
  • 克隆號：
    5C12D3
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IF, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB WB：1:1000-1:5000
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

  • 功能：
    Translocates drugs and phospholipids across the membrane. Catalyzes the flop of phospholipids from the cytoplasmic to the exoplasmic leaflet of the apical membrane. Participates mainly to the flop of phosphatidylcholine, phosphatidylethanolamine, beta-D-glucosylceramides and sphingomyelins. Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
  • 相關(guān)疾病：
    Inflammatory bowel disease 13 (IBD13)
  • 亞細(xì)胞定位：
    Cell membrane; Multi-pass membrane protein. Apical cell membrane.
  • 蛋白家族：
    ABC transporter superfamily, ABCB family, Multidrug resistance exporter (TC 3.A.1.201) subfamily
  • 組織特異性：
    Expressed in liver, kidney, small intestine and brain.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 40

    OMIM: 171050

    KEGG: hsa:5243

    STRING: 9606.ENSP00000265724

    UniGene: Hs.489033