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  4. CD48 Monoclonal Antibody

CD48 Monoclonal Antibody

  • 中文名稱：
    CD48鼠單克隆抗體
  • 貨號(hào)：
    CSB-MA004941A1m
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: Jurkat whole cell lysate, Raji whole cell lysate, Daudi whole cell lysate, Mouse liver tissue
      All lanes: CD48 antibody at 1:2000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 28, 20 KDa
      Observed band size: 43 KDa
      Exposure time:5min
    • Western Blot
      Positive WB detected in: 20μg Raji whole cell lysate
      CD48 antibody at 1:2000, 1:4000, 1:8000, 1:16000, 1:32000, 1:64000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 28, 20 KDa
      Observed band size: 43 KDa
      Exposure time:5min
    • Immunofluorescence staining of JK cells with CSB-MA004941A1m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
    • Overlay Peak curve showing JK cells stained with CSB-MA004941A1m (red line) at 1:200. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    CD48單克隆抗體（CUSABIO貨號(hào)：CSB-MA004941A1m）是針對(duì)免疫調(diào)節(jié)關(guān)鍵靶點(diǎn)CD48研發(fā)的高特異性科研工具。CD48作為SLAM家族成員，廣泛表達(dá)于淋巴細(xì)胞表面，在T細(xì)胞共刺激、NK細(xì)胞活化及抗原呈遞等免疫調(diào)控中發(fā)揮重要作用，其異常表達(dá)與腫瘤免疫逃逸、自身免疫性疾病密切相關(guān)。本產(chǎn)品采用雜交瘤技術(shù)制備，具有高親和力與人鼠交叉反應(yīng)特性，經(jīng)ELISA、Western Blot、免疫熒光及流式細(xì)胞術(shù)等多平臺(tái)驗(yàn)證，適用于多物種模型的免疫機(jī)制研究。其卓越的批間穩(wěn)定性與低背景信號(hào)特點(diǎn)，可精準(zhǔn)解析CD48在免疫突觸形成、腫瘤微環(huán)境調(diào)控中的動(dòng)態(tài)表達(dá)，為探索感染性疾病免疫應(yīng)答、腫瘤免疫治療靶點(diǎn)篩選及自身免疫病理機(jī)制提供可靠工具。該抗體在藥物開發(fā)中的機(jī)制驗(yàn)證、基因編輯模型表型鑒定及多組學(xué)聯(lián)合分析等場(chǎng)景中表現(xiàn)優(yōu)異，尤其適合需要跨平臺(tái)數(shù)據(jù)對(duì)比的綜合性研究，助力科研人員深入探索CD48介導(dǎo)的免疫調(diào)控網(wǎng)絡(luò)與疾病關(guān)聯(lián)性。
  • 產(chǎn)品名稱：
    Mouse anti-Homo sapiens (Human) CD48 Monoclonal antibody
  • Uniprot No.：
  • 基因名：
  • 別名：
    Antigen CD48 antibody; B cell membrane protein antibody; B lymphocyte activation marker BLAST 1 antibody; B-cell activation marker antibody; B-lymphocyte activation marker BLAST-1 antibody; BCM 1 surface antigen antibody; BCM1 antibody; BCM1 surface antigen antibody; BLAST 1 antibody; BLAST antibody; BLAST1 antibody; CD 48 antibody; CD48 antibody; CD48 antigen (B cell membrane protein) antibody; CD48 antigen antibody; CD48 molecule antibody; CD48 protein antibody; CD48_HUMAN antibody; hCD48 antibody; Leucocyte antigen MEM 102 antibody; Leukocyte antigen MEM-102 antibody; mCD48 antibody; MEM 102 antibody; MEM-102 antibody; MEM102 antibody; Signaling lymphocytic activation molecule 2 antibody; SLAM family member 2 antibody; SLAMF 2 antibody; SLAMF2 antibody; TCT.1 antibody
  • 宿主：
    Mouse
  • 反應(yīng)種屬：
    Human, Mouse
  • 免疫原：
    Recombinant Human CD48 protein (27-220AA)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    IgG1
  • 純化方式：
    >95%, Protein G purified
  • 克隆號(hào)：
    26C2C11
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IF, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:2000-1:64000
    IF 1:50-1:200
    FC 1:100-1:500
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Ligand for CD2. Might facilitate interaction between activated lymphocytes. Probably involved in regulating T-cell activation.
  • 基因功能參考文獻(xiàn)：
    1. soluble CD48 levels were significantly elevated in patients with nonallergic asthma compared to control and to the allergic asthma cohort PMID: 30306094
    2. mCD48 and sCD48 are differentially expressed in the peripheral blood of asthma patients of varying severity. sCD48 inhibits CD244-mediated eosinophil activation. These findings suggest that CD48 may play an important role in human asthma. PMID: 27859399
    3. The present study provides further insights into the role of the 2B4-CD48 interaction in the fine regulation of CD8(+) T-cell effector function upon antigenic stimulation. PMID: 26860368
    4. Data show that 2B4 not only can bind to CD48 in trans but also interacts with CD48 in cis by using the same binding interface. Also, the results demonstrated that constitutive phosphorylation of 2B4 occurs only in the presence of CD48, and that cis binding is sufficient to induce substantial levels of baseline phosphorylation. PMID: 27249817
    5. Our data indicate sCD48 as a SEB-induced 'decoy' receptor derived from eosinophil and therefore as a potential anti-inflammatory tool in S. aureus-induced eosinophil inflammation often associated with allergy. PMID: 26836239
    6. CD48 expression was increased in patients with a short disease duration compared to both controls and patients with longer disease duration. In patients with short disease duration, increased CD48 expression was associated with alveolar inflammation. PMID: 26926492
    7. These data demonstrate the important role of CD48 in SA/exotoxins-eosinophil activating interactions that can take place during allergic responses and indicate CD48 as a novel therapeutic target for allergy and especially of AD. PMID: 25255823
    8. Blockade of 2B4/CD48 interaction resulted in improvement in function via perforin expression and degranulation as measured by CD107a surface mobilization on HTLV-1 specific CD8+ T cells. PMID: 24505299
    9. we propose that SLAMF2 engagement regulates adaptive immune responses PMID: 24670806
    10. Monocyte-induced natural killer cell dysfunction was markedly attenuated by blocking CD48 receptor 2B4 on NK cells, but not by blockade of NKG2D and NKp30. PMID: 23225218
    11. replication study of association of 2 SNPs in HERV-K18 and 19 tagSNPs in CD48 with schizophrenia (SZ)and type 2 diabetes (T2D) in patients with SZ in 2 Danish samples; no association was found with SZ or with T2D among individuals with SZ for any of the SNPs PMID: 22495247
    12. Stimulation of CD48 induces rearrangement of signaling factors in lipid rafts, Lck-kinase activity, and tyrosine phosphorylation. PMID: 20833258
    13. The ligand (CD48) of the 2B4 receptor can exert both activating and inhibiting signals; natural killer (NK) cells might be at risk for self-killing were it not for the inhibiting signals generated by the 2B4-CD48 interaction. PMID: 20164429
    14. CM1-induced apoptosis is achieved via different initiation pathways, which are cell-type dependent PMID: 12072193
    15. Signal-dependent adhesion of resting NK cells initiated by expression of ICAM-1 is greatly enhanced by coexpression of CD48, even in the absence of cytokines. PMID: 12496412
    16. Engagement of natural killer (NK) cell receptor 2B4 by its counterreceptor, CD48, expressed on target cells leads to an inhibition in NK cytotoxicity independent of signaling lymphocytic activation molecule-associated protein (SAP) expression. PMID: 15356144
    17. IL-18, IL-18 receptor alpha, and CD48 complex formation via glycosylphosphatidylinositol anchor glycan triggers binding to IL-18 receptor beta, and thereby induces intracellular signal transduction and IFN-gamma production. PMID: 15760905
    18. Review of recent studies suggests an important role for interactions between 2B4 and CD48 in the course of T cell activation and proliferation PMID: 16081768
    19. 2B4 (CD244) can stimulate NK cell cytotoxicity and cytokine production by interacting with NK cell expressed CD48 and adds CD48 to the growing number of activating NK cell receptors PMID: 16585556
    20. CD48 is an interleukin (IL)-3-induced activating receptor on eosinophils and may be involved in promoting allergic inflammation. PMID: 16785501
    21. In conclusion, we cannot confirm a role of human endogenous retrovirus-K18 superantigen polymorphisms or of the CD48 CA repeat for type 1 diabetes susceptibility. PMID: 16866884
    22. findings indicate that FimH induces host cell signalling cascades that are involved in E. coli K1 invasion of human brain microvascular endothelial cells (HBMEC) and CD48 is a putative HBMEC receptor for FimH PMID: 17222190
    23. the mechanism of signal transduction by CD244 is to regulate FYN kinase recruitment and/or activity and the outcome of CD48/CD244 interactions is determined by which other receptors are engaged. PMID: 17599905

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  • 亞細(xì)胞定位：
    Cell membrane; Lipid-anchor, GPI-anchor.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 1683

    OMIM: 109530

    KEGG: hsa:962

    STRING: 9606.ENSP00000357025

    UniGene: Hs.243564