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Enterobacteria phage M13 III Monoclonal Antibody

Rare Species
  • 中文名稱：
    大腸桿菌噬菌體M13 III鼠單克隆抗體
  • 貨號(hào)：
    CSB-MA303116A0m
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: 1-3 lanes: Recombinant Enterobacteria phage M13 Attachment protein at 5ng, 10ng, 20ng; 4 lane: Enterobacteria phage M13 Attachment
      All lanes: Enterobacteria phage M13 III antibody at 1:1000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 65, 55-70 KDa
      Observed band size: 65, 55-70 KDa
      Exposure time：3min
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    Enterobacteria phage M13 III Monoclonal Antibody（CUSABIO貨號(hào)：CSB-MA303116A0m）是一款高特異性科研用抗體，靶向M13噬菌體的III型結(jié)構(gòu)蛋白。M13噬菌體作為絲狀噬菌體家族代表，其衣殼蛋白在基因工程領(lǐng)域具有重要價(jià)值，廣泛用于噬菌體展示技術(shù)構(gòu)建重組蛋白表達(dá)系統(tǒng)。該抗體采用雜交瘤技術(shù)制備，經(jīng)嚴(yán)格驗(yàn)證可特異性識(shí)別Enterobacteria phage M13的III型蛋白表位，在ELISA實(shí)驗(yàn)中展現(xiàn)高親和力結(jié)合特性，適用于Western Blot檢測(cè)重組噬菌體蛋白表達(dá)水平。產(chǎn)品經(jīng)過(guò)交叉反應(yīng)性測(cè)試，與大腸桿菌宿主蛋白無(wú)交叉反應(yīng)，保障實(shí)驗(yàn)特異性，適用于噬菌體文庫(kù)篩選、重組蛋白表達(dá)驗(yàn)證等分子生物學(xué)研究場(chǎng)景。作為噬菌體展示技術(shù)的關(guān)鍵檢測(cè)工具，本產(chǎn)品可高效支持抗體庫(kù)構(gòu)建、靶向肽篩選及病毒樣顆粒（VLP）研究，為基因工程、表位定位和蛋白質(zhì)相互作用研究提供可靠試劑。實(shí)驗(yàn)方案已優(yōu)化適配常規(guī)檢測(cè)體系，滿足科研人員對(duì)M13噬菌體相關(guān)實(shí)驗(yàn)的精準(zhǔn)檢測(cè)需求，是分子克隆與蛋白工程研究的理想選擇。
  • 產(chǎn)品名稱：
    Mouse anti-Enterobacteria phage M13 III Monoclonal antibody
  • Uniprot No.：
  • 基因名：
    III
  • 別名：
    III antibody; Attachment protein G3P antibody; Gene 3 protein antibody; G3P antibody; Minor coat protein antibody
  • 宿主：
    Mouse
  • 反應(yīng)種屬：
    Enterobacteria phage M13
  • 免疫原：
    Recombinant Enterobacteria phage M13 Attachment protein (19-424AA)
  • 免疫原種屬：
    Enterobacteria phage M13
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    IgG1
  • 純化方式：
    >95%, Protein G purified
  • 克隆號(hào)：
    1B8E8
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:5000
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Plays essential roles both in the penetration of the viral genome into the bacterial host via pilus retraction and in the extrusion process. During the initial step of infection, G3P mediates adsorption of the phage to its primary receptor, the tip of host F-pilus. Subsequent interaction with the host entry receptor tolA induces penetration of the viral DNA into the host cytoplasm. In the extrusion process, G3P mediates the release of the membrane-anchored virion from the cell via its C-terminal domain.
  • 亞細(xì)胞定位：
    Virion. Host membrane; Single-pass type I membrane protein. Note=Prior to assembly, G3P is found associated with the bacterial host inner membrane. There are about five copies of this protein per mature phage that are located on the head side of the filamentous virion.
  • 蛋白家族：
    Inovirus G3P protein family
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: vg:927334