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SLN Monoclonal Antibody

  • 中文名稱：
    SLN鼠單克隆抗體
  • 貨號(hào)：
    CSB-MA021780A0m
  • 規(guī)格：
    ￥1320
  • 圖片：
    • IHC image of CSB-MA021780A0m diluted at 1:60 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Overlay Peak curve showing U87 cells stained with CSB-MA021780A0m (red line) at 1:60. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    CUSABIO貨號(hào)：CSB-MA021780A0m SLN單克隆抗體是針對(duì)肌集鈣蛋白（Sarcolipin）靶點(diǎn)開發(fā)的高特異性科研試劑，該蛋白作為調(diào)節(jié)肌漿網(wǎng)鈣ATP酶活性的關(guān)鍵因子，在骨骼肌和心肌細(xì)胞的鈣離子穩(wěn)態(tài)調(diào)控中發(fā)揮重要作用。本產(chǎn)品采用雜交瘤技術(shù)制備，經(jīng)ELISA驗(yàn)證具有高親和力，可兼容免疫組化（IHC）進(jìn)行組織定位分析及流式細(xì)胞術(shù)（FC）檢測細(xì)胞表面表達(dá)，適用于人類樣本的蛋白質(zhì)功能研究。其明確的物種特異性設(shè)計(jì)有效避免了交叉反應(yīng)風(fēng)險(xiǎn)，為研究肌肉代謝機(jī)制、能量平衡調(diào)控及相關(guān)疾病模型構(gòu)建提供可靠工具。在運(yùn)動(dòng)生理學(xué)、代謝性疾病基礎(chǔ)研究及心肌功能探索等科研領(lǐng)域，該抗體可廣泛應(yīng)用于蛋白互作分析、亞細(xì)胞定位研究和分子信號(hào)通路解析，幫助科研人員深入探究SLN在肌細(xì)胞收縮舒張、產(chǎn)熱調(diào)節(jié)中的分子機(jī)制。
  • 產(chǎn)品名稱：
    Mouse anti-Homo sapiens (Human) SLN Monoclonal antibody
  • Uniprot No.：
  • 基因名：
    SLN
  • 別名：
    SLN antibody; Sarcolipin antibody
  • 宿主：
    Mouse
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    Recombinant Human SLN protein (1-31AA)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    IgG2b
  • 純化方式：
    >95%, Protein G purified
  • 克隆號(hào)：
    14G1A12
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IF, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB WB：1:1000-1:5000
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Reversibly inhibits the activity of ATP2A1 and ATP2A2 in sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+). Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in muscle. Required for muscle-based, non-shivering thermogenesis.
  • 基因功能參考文獻(xiàn)：
    1. Structure-Function Relationship of the SERCA Pump and Its Regulation by Phospholamban and Sarcolipin. PMID: 29594859
    2. Self-assembling study of sarcolipin and its mutants in multiple molecular dynamic simulations has been reported. PMID: 28241400
    3. These results suggest that sAnk1 interacts with SLN both directly and in complex with SERCA1 and reduces SLN's inhibitory effect on SERCA1 activity. PMID: 28487373
    4. analysis of transmembrane dynamics of the Thr-5 phosphorylated sarcolipin pentameric channel PMID: 27378083
    5. Phospholamban and sarcolipin are membrane proteins that differentially regulate SERCA function. (Review) PMID: 26743715
    6. Molecular dynamics simulations help to clarify and to understand better the SLN pentamer channel that had a hydrophobic gate and could switch Na(+) and Cl(-) ion permeability by hydrated and vacuum states. PMID: 26522287
    7. Although SLN and PLB binding to SERCA have different functional outcomes on the coupling efficiency of SERCA, both proteins decrease the apparent Ca(2+) affinity of the pump, suggesting that SLN and PLB inhibit SERCA by using a similar mechanism. PMID: 25983321
    8. SLN and PLN are co-expressed in most fibers, which suggests that super-inhibition of SERCAs may be physiologically important in the regulation of intracellular Ca2+ in human skeletal muscle. PMID: 24358354
    9. The C-terminal tail of SLN is a distinct, essential domain in the regulation of SERCA. PMID: 23362265
    10. The selective downregulation of SLN and enhanced sarcoplasmic reticulum Ca(2+) uptake in human AF suggest that SLN downregulation could play an important role in abnormal intracellular Ca(2+) cycling in atrial pathology. PMID: 21640081
    11. sarcolipin binds to phospholamban and inhibits polymerization PMID: 12032137
    12. sarcolipin is likely to be an atrial chamber-specific regulator of Ca2+ cycling in heart PMID: 12645548
    13. role in regulating sarco(endo)plasmic reticulum Ca2+-ATPase by binding to transmembrane helices alone or in association with phospholamban PMID: 12692302
    14. Gene expression of SLN was studied in children with congenital heart defects. PMID: 17515962

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  • 亞細(xì)胞定位：
    Sarcoplasmic reticulum membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein.
  • 蛋白家族：
    Sarcolipin family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 11089

    OMIM: 602203

    KEGG: hsa:6588

    STRING: 9606.ENSP00000304707

    UniGene: Hs.334629