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USP6 Monoclonal Antibody

  • 中文名稱：
    USP6鼠單克隆抗體
  • 貨號(hào)：
    CSB-MA025747A0m
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: USP6 antibody at 1:1000
      Lane 1: MCF-7 whole cell lysate
      Lane 2: THP-1 whole cell lysate
      Lane 3: SH-SY5Y whole cell lysate
      Lane 4: Jurkat whole cell lysate
      Lane 5: HEK293 whole cell lysate
      Lane 6: Hela whole cell lysate
      Secondary
      Goat polyclonal to Mouse IgG at 1/20000 dilution
      Predicted band size: 89, 122, 159 KDa
      Observed band size: 89, 122, 159 KDa
      Exposure time: 1min
    • Immunofluorescence staining of HeLa cells with CSB-MA025747A0m at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    USP6單克隆抗體（CUSABIO貨號(hào)：CSB-MA025747A0m）是針對(duì)泛素特異性蛋白酶6靶點(diǎn)研發(fā)的高特異性科研試劑，采用重組人源USP6蛋白免疫動(dòng)物制備而成，具備精準(zhǔn)識(shí)別人類USP6蛋白的能力。該抗體經(jīng)過嚴(yán)格驗(yàn)證，可穩(wěn)定應(yīng)用于酶聯(lián)免疫吸附實(shí)驗(yàn)（ELISA）、蛋白質(zhì)印跡（WB）和免疫熒光（IF）三大核心檢測(cè)平臺(tái)，適用于腫瘤發(fā)生機(jī)制研究、泛素-蛋白酶體系統(tǒng)調(diào)控分析及細(xì)胞信號(hào)轉(zhuǎn)導(dǎo)通路探索等科研領(lǐng)域。作為泛素化修飾研究的關(guān)鍵工具，該產(chǎn)品能有效檢測(cè)內(nèi)源性USP6蛋白表達(dá)水平，助力研究人員解析其在蛋白質(zhì)降解調(diào)控、細(xì)胞周期進(jìn)程中的分子作用機(jī)制。本產(chǎn)品嚴(yán)格遵循科研用途標(biāo)準(zhǔn)，為癌癥生物學(xué)、分子病理學(xué)等基礎(chǔ)研究提供可靠實(shí)驗(yàn)支持，特別適用于腫瘤樣本的蛋白組學(xué)分析、疾病相關(guān)信號(hào)通路驗(yàn)證等前沿課題研究。
  • 產(chǎn)品名稱：
    Human anti-Homo sapiens (Human) USP6 Monoclonal antibody
  • Uniprot No.：
  • 基因名：
    USP6
  • 別名：
    Deubiquitinating enzyme 6 antibody; HRP1 antibody; Proto-oncogene TRE-2 antibody; TRE17 antibody; TRE2 antibody; Ubiquitin carboxyl-terminal hydrolase 6 antibody; Ubiquitin specific protease 6 antibody; Ubiquitin thiolesterase 6 antibody; Ubiquitin-specific-processing protease 6 antibody; UBP6_HUMAN antibody; USP6 antibody
  • 宿主：
    Human
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    Recombinant Human Ubiquitin carboxyl-terminal hydrolase 6 protein (348-535AA)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    IgG1
  • 純化方式：
    >95%, Protein G purified
  • 克隆號(hào)：
    3C3G2
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IF
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    IF 1:50-1:200
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Deubiquitinase with an ATP-independent isopeptidase activity, cleaving at the C-terminus of the ubiquitin moiety. Catalyzes its own deubiquitination. In vitro, isoform 2, but not isoform 3, shows deubiquitinating activity. Promotes plasma membrane localization of ARF6 and selectively regulates ARF6-dependent endocytic protein trafficking. Is able to initiate tumorigenesis by inducing the production of matrix metalloproteinases following NF-kappa-B activation.
  • 基因功能參考文獻(xiàn)：
    1. None of the genitourinary pseudosarcomatous myofibroblastic proliferations was found to harbour USP6 (0/12), ROS1 (0/8) or ETV6 (0/7) rearrangements PMID: 29617048
    2. we identified seven novel fusion partners for USP6 in nodular fasciitis, highlighting the importance of USP6 expression and promoter-swapping fusions in the etiology of this neoplasm PMID: 28752842
    3. Report the presence of USP6 rearrangements in a subset of cellular fibroma of tendon sheath. PMID: 27125357
    4. our studies highlight Jak1 as the first identified substrate for USP6, and they offer a mechanistic rationale for the clinical investigation of Jak and STAT3 inhibitors as therapeutics for the treatment of bone and soft tissue tumors along with other neoplasms driven by USP6 overexpression PMID: 27440725
    5. Molecular analyses revealed the presence and amplification of the novel PPPR6-USP6 gene fusion, which resulted in USP6 mRNA transcriptional upregulation. These findings further support the oncogenic role of the USP6 protease in mesenchymal neoplasia and expand the biologic potential of Nodular fasciitis PMID: 27113271
    6. It was shown that TRE17 activates the classical NF-kappa B pathway through an atypical mechanism that does not involve IkappaB degradation. Optimal activation of NF-kappa B by TRE17 required both catalytic subunits of IkappaB kinase. PMID: 22081069
    7. USP6 fluorescence in-situ hybridization is a useful ancillary test in cases where nodular fasciitis is a potential diagnostic consideration. PMID: 27271298
    8. the deubiquitylase ubiquitin-specific protease 6 (USP6) as a potent activator of Wnt signaling. USP6 enhances Wnt signaling by deubiquitylating Fzds, thereby increasing their cell-surface abundance. PMID: 27162353
    9. TRE17/USP6 regulates ubiquitylation and trafficking of cargo proteins that enter cells by clathrin-independent endocytosis PMID: 25179595
    10. 8 of the 9 giant cell reparative granulomas from hands and feet showed rearrangements of the USP6 gene compared with none of 8 gnathic lesions PMID: 24742829
    11. we discuss the clinicopathologic features, molecular pathology, and pathogenesis of ABC and nodular fasciitis in relation to USP6 PMID: 23769422
    12. identification of a USP6 gene rearrangement is helpful in making a diagnosis of nodular fasciitis. PMID: 23748914
    13. manipulating USP6 expression levels alters the ability of cells to migrate and to divide. Cell proliferation and progression through cytokinesis depend on USP6 expression PMID: 22188517
    14. TRE17 is sufficient to initiate tumorigenesis, identify MMPs as novel TRE17 effectors that likely contribute to aneurysmal bone cyst pathogenesis. PMID: 20418905
    15. TRE17 coprecipitated specifically with the active forms of Cdc42 and Rac1 in vivo. TRE17 is part of a novel effector complex for Cdc42 and Rac1, potentially contributing to their effects on actin remodeling. PMID: 12612085
    16. Complementation tests in yeasts indicate that Tre2 codes for a nonfunctional RabGAP. PMID: 14521938
    17. Deregulated USP6 transcription is associated with aneurysmal bone cyst PMID: 15026324
    18. primary aneurysmal bone cysts are mesenchymal neoplasms exhibiting USP6 and/or CDH11 oncogenic rearrangements PMID: 15509545
    19. TRE17 associates directly with Arf6 in its GDP- but not GTP-bound state. PMID: 15509780
    20. Tre2 oncogene seems to encode a nonfunctional Rab GAP. As regions flanking the TBC domain may be crucial for catalytic activity PMID: 16099424
    21. Ca2+/CaM has a role in regulating ubiquitination through direct interaction with TRE17 PMID: 16127172
    22. The lack of secondary structure of the region flanking the TBC domain in TRE2 may explain why this region plays a role in the lack of GAP activity, even when a potentially functional TBC domain is present. PMID: 17701273
    23. No USP6 rearrangements were found in cherubism or brown tumors. USP6 rearrangements were identified in 2 patients with myositis ossificans. PMID: 18265974

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  • 相關(guān)疾?。?/div>
    A chromosomal aberration involving USP6 is a common genetic feature of aneurysmal bone cyst, a benign osseous neoplasm. Translocation t(16;17)(q22;p13) with CDH11. The translocation generates a fusion gene in which the strong CDH11 promoter is fused to the entire USP6 coding sequence, resulting in USP6 transcriptional up-regulation (PubMed:15026324).
  • 亞細(xì)胞定位：
    Cell membrane. Cytoplasm. Endosome. Note=Localizes to the plasma membrane and to filamentous structures within the cell corresponding to ARF6 regulated tubular endosomes. Activation of RAC1 and CDC42 can direct the relocalization of USP6 to the plasma membrane in a manner that depends on the integrity of the actin cytoskeleton.
  • 蛋白家族：
    Peptidase C19 family
  • 組織特異性：
    Testis specific. Expressed in various cancer cell lines.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 12629

    OMIM: 604334

    KEGG: hsa:9098

    STRING: 9606.ENSP00000250066

    UniGene: Hs.448851