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lacZ Monoclonal Antibody

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  • 中文名稱：
    lacZ鼠單克隆抗體
  • 貨號(hào)：
    CSB-MA009476A0m
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in Recombinant protein
      All lanes: lacZ antibody at 1:2000, 1:4000, 1:8000, 1:16000, 1:32000, 1:64000
      Secondary
      Goat polyclonal to Mouse IgG at 1/50000 dilution
      Predicted band size: 117 kDa
      Observed band size: 130 kDa
    • Western Blot
      Positive WB detected in Recombinant protein (10ng, 5ng, 2.5ng, 1.25ng, 0.625ng)
      All lanes: lacZ antibody at 1:2000
      Secondary
      Goat polyclonal to Mouse IgG at 1/50000 dilution
      Predicted band size: 117 kDa
      Observed band size: 130 kDa
    • Immunofluorescence staining of 293T cells transfected with CSB-MA009476A0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). The image on the right is the 293T cells transfected without lacZ antibody
    • Immunoprecipitating lacZ in 293T transfected whole cell lysate
      Lane 1: Mouse control IgG instead of CSB-MA009476A0m in 293T transfected whole cell lysate
      Lane 2: CSB-MA009476A0m (2μl) + 293T transfected whole cell lysate (500μg)
      Lane 3: 293T transfected whole cell lysate (20μg)
      For western blotting, the blot was detected with CSB-MA000021M0m at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000
    • Overlay histogram showing 293T transfected cells (red line) or untransfected cells (green line) stained with CSB-MA009476A0m at 1:200. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    CUSABIO貨號(hào)：CSB-MA009476A0m單克隆抗體是專為大腸桿菌lacZ基因編碼的β-半乳糖苷酶檢測(cè)開發(fā)的科研工具，該酶在乳糖代謝中負(fù)責(zé)水解乳糖為可代謝單糖，同時(shí)作為分子生物學(xué)中廣泛應(yīng)用的報(bào)告基因標(biāo)記。本產(chǎn)品采用雜交瘤技術(shù)制備，可特異性識(shí)別β-半乳糖苷酶抗原表位，適用于ELISA、Western Blot、免疫熒光、免疫沉淀及流式細(xì)胞術(shù)等多平臺(tái)檢測(cè)。在基因工程領(lǐng)域，該抗體可用于重組質(zhì)粒篩選驗(yàn)證，通過(guò)特異性識(shí)別β-半乳糖苷酶表達(dá)產(chǎn)物輔助藍(lán)白斑篩選系統(tǒng)；在蛋白相互作用研究中，可配合免疫共沉淀技術(shù)解析酶復(fù)合體形成機(jī)制；其流式檢測(cè)功能為細(xì)菌代謝狀態(tài)分析提供新維度。該抗體還可應(yīng)用于基因表達(dá)調(diào)控研究，通過(guò)Western Blot定量分析lacZ報(bào)告基因在不同條件下的表達(dá)水平，為啟動(dòng)子活性檢測(cè)和基因治療載體構(gòu)建提供關(guān)鍵技術(shù)支持。
  • 產(chǎn)品名稱：
    Mouse anti-Escherichia coli lacZ Monoclonal antibody
  • Uniprot No.：
  • 基因名：
    lacZ
  • 別名：
    lacZ antibody; b0344 antibody; JW0335Beta-galactosidase antibody; Beta-gal antibody; EC 3.2.1.23 antibody; Lactase antibody
  • 宿主：
    Mouse
  • 反應(yīng)種屬：
    Escherichia coli
  • 免疫原：
    Recombinant Escherichia coli Beta-galactosidase protein (2-1024AA)
  • 免疫原種屬：
    Escherichia coli
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    IgG1
  • 純化方式：
    >95%, Protein G purified
  • 克隆號(hào)：
    3B7D5
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IF, IP, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:64000
    IF 1:50-1:200
    IP 1ul-4ul
    FC 1:100-1:300
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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