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  4. Recombinant Human Insulin growth factor-like family member 1 (IGFL1) (Active)

Recombinant Human Insulin growth factor-like family member 1 (IGFL1) (Active)

In Stock
  • 中文名稱：
    人IGFL1重組蛋白
  • 貨號(hào)：
    CSB-MP764932HU
  • 規(guī)格：
    ￥828
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Activity
      Measured by its binding ability in a functional ELISA. Immobilized Human IGFLR1 (CSB-MP862025HU) at 2 μg/ml can bind Human IGFL1, the EC50 is 32.33-47.52 ng/mL. Biological Activity Assay
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 95% as determined by SDS-PAGE.
  • 內(nèi)毒素：
    Less than 1.0 EU/ug as determined by LAL method.
  • 生物活性：
    Measured by its binding ability in a functional ELISA. Immobilized Human IGFLR1 (CSB-MP862025HU) at 2 μg/mL can bind Human IGFL1, the EC50 is 32.33-47.52 ng/mL.
  • 基因名：
  • Uniprot No.：
  • 分子結(jié)構(gòu)：
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長(zhǎng)度：
    Full Length of Mature Protein
  • 來(lái)源：
    Mammalian cell
  • 分子量：
    38.7 kDa
  • 表達(dá)區(qū)域：
    25-110aa
  • 氨基酸序列
    APVAPMTPYLMLCQPHKRCGDKFYDPLQHCCYDDAVVPLARTQTCGNCTFRVCFEQCCPWTFMVKLINQNCDSARTSDDRLCRSVS
  • 蛋白標(biāo)簽：
    C-terminal hFc1-tagged
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產(chǎn)品描述：

    Recombinant human IGFL1 protein is produced by a mammalian expression system. The target protein is expressed with a DNA fragment (Ala25-Ser110) of human IGFL1 fused with a hFc tag at the C-terminus. This active IGFL1 protein has been measured its activity in a functional ELISA. Immobilized human IGFLR1 binds to this IGFL1 protein with EC50 of 32.33-47.52 ng/mL. The SDS-PAGE showed that the purity of this IGFL1 protein was greater than 95%. The IGFL1 protein has an apparent molecular weight of approximately 40 kDa due to glycosylation. The LAL method showed that the protein contained endotoxin less than 1.0 EU/ug. This human IGFL1 protein is in stock now.

    IGFL1 is expressed in cancer cell lines, fetal skin, head and neck tumors, normal breast, squamous cell carcinoma, and uterine tumors. IGFL1 expression is up-regulated in human psoriasis skin. Studies showed that human IGFL1 produced may regulate T cell response. Xiaorong Ji et al. found that high IGFL1 expression can be used as an independent prognostic factor of serous ovarian carcinoma.

  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

平均分:
5.0分 - 1 個(gè)評(píng)價(jià)

實(shí)驗(yàn)應(yīng)用：結(jié)合實(shí)驗(yàn)/蛋白間互作

產(chǎn)品評(píng)價(jià)： 我使用CSB-MP764932HU的IGFL1與購(gòu)買的另一個(gè)產(chǎn)品Human IGFLR1 (CSB-MP862025HU)進(jìn)行功能ELISA實(shí)驗(yàn)，顯示有較好的結(jié)合活性，EC50為4.640-5.722?ng/mL。不同批次之間，差異不顯著，重復(fù)性好，更值得一提的是售后服務(wù)好，加分~后續(xù)還會(huì)繼續(xù)購(gòu)買~~

By 鞠老師

靶點(diǎn)詳情

  • 功能：
    Probable ligand of the IGFLR1 cell membrane receptor.
  • 基因功能參考文獻(xiàn)：
    1. Murine insulin growth factor-like (IGFL) and human IGFL1 proteins are induced in inflammatory skin conditions and bind to a novel tumor necrosis factor receptor family member, IGFLR1. PMID: 21454693
    2. The -1245 A-allele of the IGF1 promoter single nucleotide polymorphism is associated with a small head size and less brain sparing in small for gestational age born subjects PMID: 19147602
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    IGFL family
  • 組織特異性：
    Detected in ovary and spinal cord.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 24093

    OMIM: 610544

    KEGG: hsa:374918

    UniGene: Hs.546554