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  4. ARFGAP3 Antibody

ARFGAP3 Antibody

  • 中文名稱：
    ARFGAP3兔多克隆抗體
  • 貨號：
    CSB-PA885676ESR1HU
  • 規(guī)格：
    ￥440
  • 圖片：
    • Western blot
      All lanes: ARFGAP3 antibody at 4μg/ml
      Lane 1: HepG2 whole cell lysate
      Lane 2: Jurkat whole cell lysate
      Lane 3: A549 whole cell lysate
      Secondary
      Goat polyclonal to rabbit IgG at 1/10000 dilution
      Predicted band size: 57, 52 kDa
      Observed band size: 57 kDa
    • Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA885676ESR1HU at dilution of 1:100
    • Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA885676ESR1HU at dilution of 1:100
    • Immunoprecipitating ARFGAP3 in HepG2 whole cell lysate
      Lane 1: Rabbit control IgG instead of (1μg) instead of CSB-PA885676ESR1HU in HepG2 whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)
      Lane 2: CSB-PA885676ESR1HU (4μg) + HepG2 whole cell lysate (500μg)
      Lane 3: HepG2 whole cell lysate (20μg)
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品名稱：
    Rabbit anti-Homo sapiens (Human) ARFGAP3 Polyclonal antibody
  • Uniprot No.：
  • 基因名：
  • 別名：
    ADP ribosylation factor GTPase activating protein 1 antibody; ADP ribosylation factor GTPase activating protein 3 antibody; ADP-ribosylation factor GTPase-activating protein 3 antibody; ARF GAP 3 antibody; ARFG3_HUMAN antibody; ArfGAP 3 antibody; ARFGAP1 antibody; ARFGAP3 antibody; FLJ45618 antibody
  • 宿主：
    Rabbit
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    Recombinant Human ADP-ribosylation factor GTPase-activating protein 3 protein (217-516AA)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    Antigen Affinity Purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IHC, IP
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    IHC 1:20-1:200
    IP 1:200-1:2000
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

  • 功能：
    GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes.
  • 基因功能參考文獻：
    1. We suggest a model in which ArfGAP3 regulates Golgi association of GGA clathrin adaptors. PMID: 24076238
    2. our results suggest that ARFGAP3 is a novel androgen-regulated gene that can promote prostate cancer cell proliferation and migration in collaboration with paxillin. PMID: 21647875
    3. human ARFGAP2 and ARFGAP3 are associated with COP-I-coated vesicles and function in COP I traffic PMID: 17760859
    4. Differential roles of ArfGAP1, ArfGAP2, and ArfGAP3 in COPI trafficking PMID: 19015319
    5. ArfGAP1, ArfGAP2, and ArfGAP3 have overlapping roles in regulating COPI function in Golgi-to-ER retrograde transport. PMID: 19299515

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  • 亞細胞定位：
    Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Note=Also found on peripheral punctate structures likely to be endoplasmic reticulum-Golgi intermediate compartment.
  • 組織特異性：
    Widely expressed. Highest expression in endocrine glands (pancreas, pituitary gland, salivary gland, and prostate) and testis with a much higher expression in the testis than in the ovary.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 661

    OMIM: 612439

    KEGG: hsa:26286

    STRING: 9606.ENSP00000263245

    UniGene: Hs.685225