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ATP6V0D2 Antibody

  • 中文名稱(chēng):
    ATP6V0D2兔多克隆抗體
  • 貨號(hào):
    CSB-PA818735LA01HU
  • 規(guī)格:
    ¥440
  • 圖片:
    • Western Blot
      Positive WB detected in: A549 whole cell lysate, PC3 whole cell lysate
      All lanes: ATP6V0D2 antibody at 1:2000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 41 kDa
      Observed band size: 41 kDa
    • IHC image of CSB-PA818735LA01HU diluted at 1:500 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of HepG2 cells with CSB-PA818735LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品名稱(chēng):
    Rabbit anti-Homo sapiens (Human) ATP6V0D2 Polyclonal antibody
  • Uniprot No.:
  • 基因名:
  • 別名:
    ATP6V0D2V-type proton ATPase subunit d 2 antibody; V-ATPase subunit d 2 antibody; Vacuolar proton pump subunit d 2 antibody
  • 宿主:
    Rabbit
  • 反應(yīng)種屬:
    Human
  • 免疫原:
    Recombinant Human V-type proton ATPase subunit d 2 protein (50-295AA)
  • 免疫原種屬:
    Homo sapiens (Human)
  • 標(biāo)記方式:
    Non-conjugated

    本頁(yè)面中的產(chǎn)品,ATP6V0D2 Antibody (CSB-PA818735LA01HU),的標(biāo)記方式是Non-conjugated。對(duì)于ATP6V0D2 Antibody,我們還提供其他標(biāo)記。見(jiàn)下表:

    可提供標(biāo)記
    標(biāo)記方式 貨號(hào) 產(chǎn)品名稱(chēng) 應(yīng)用
    HRP CSB-PA818735LB01HU ATP6V0D2 Antibody, HRP conjugated ELISA
    FITC CSB-PA818735LC01HU ATP6V0D2 Antibody, FITC conjugated
    Biotin CSB-PA818735LD01HU ATP6V0D2 Antibody, Biotin conjugated ELISA
  • 克隆類(lèi)型:
    Polyclonal
  • 抗體亞型:
    IgG
  • 純化方式:
    >95%, Protein G purified
  • 濃度:
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液:
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 產(chǎn)品提供形式:
    Liquid
  • 應(yīng)用范圍:
    ELISA, WB, IHC, IF
  • 推薦稀釋比:
    Application Recommended Dilution
    WB 1:1000-1:5000
    IHC 1:500-1:1000
    IF 1:50-1:200
  • Protocols:
  • 儲(chǔ)存條件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途:
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能:
    Subunit of the integral membrane V0 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system. May play a role in coupling of proton transport and ATP hydrolysis.
  • 基因功能參考文獻(xiàn):
    1. gammadelta T cells suppressed iDCs osteoclastogenesis by downregulation of the RANK/cFos/ATP6V0D2 signaling pathway. PMID: 30066839
    2. The secondary structures of d1 and d2 subunits were highly similar, but the relative stability against thermal stress was higher for d1 than d2. PMID: 24631925
    3. these data suggest that Adrm1, a new Atp6v0d2-interacting protein, plays an important role in osteoclast differentiation, and in particular the fusion of preosteoclasts. PMID: 19818731
  • 蛋白家族:
    V-ATPase V0D/AC39 subunit family
  • 組織特異性:
    Kidney, osteoclast and lung.
  • 數(shù)據(jù)庫(kù)鏈接:

    HGNC: 18266

    KEGG: hsa:245972

    STRING: 9606.ENSP00000285393

    UniGene: Hs.436360