ATP6V0D2 Antibody
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中文名稱(chēng):ATP6V0D2兔多克隆抗體
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貨號(hào):CSB-PA818735LA01HU
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規(guī)格:¥440
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圖片:
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Western Blot
Positive WB detected in: A549 whole cell lysate, PC3 whole cell lysate
All lanes: ATP6V0D2 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa -
IHC image of CSB-PA818735LA01HU diluted at 1:500 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunofluorescence staining of HepG2 cells with CSB-PA818735LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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其他:
產(chǎn)品詳情
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產(chǎn)品名稱(chēng):Rabbit anti-Homo sapiens (Human) ATP6V0D2 Polyclonal antibody
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Uniprot No.:
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基因名:
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別名:ATP6V0D2V-type proton ATPase subunit d 2 antibody; V-ATPase subunit d 2 antibody; Vacuolar proton pump subunit d 2 antibody
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宿主:Rabbit
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反應(yīng)種屬:Human
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免疫原:Recombinant Human V-type proton ATPase subunit d 2 protein (50-295AA)
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免疫原種屬:Homo sapiens (Human)
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標(biāo)記方式:Non-conjugated
本頁(yè)面中的產(chǎn)品,ATP6V0D2 Antibody (CSB-PA818735LA01HU),的標(biāo)記方式是Non-conjugated。對(duì)于ATP6V0D2 Antibody,我們還提供其他標(biāo)記。見(jiàn)下表:
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克隆類(lèi)型:Polyclonal
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抗體亞型:IgG
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純化方式:>95%, Protein G purified
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濃度:It differs from different batches. Please contact us to confirm it.
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保存緩沖液:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 -
產(chǎn)品提供形式:Liquid
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應(yīng)用范圍:ELISA, WB, IHC, IF
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推薦稀釋比:
Application Recommended Dilution WB 1:1000-1:5000 IHC 1:500-1:1000 IF 1:50-1:200 -
Protocols:
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儲(chǔ)存條件:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期:Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途:For Research Use Only. Not for use in diagnostic or therapeutic procedures.
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能:Subunit of the integral membrane V0 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system. May play a role in coupling of proton transport and ATP hydrolysis.
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基因功能參考文獻(xiàn):
- gammadelta T cells suppressed iDCs osteoclastogenesis by downregulation of the RANK/cFos/ATP6V0D2 signaling pathway. PMID: 30066839
- The secondary structures of d1 and d2 subunits were highly similar, but the relative stability against thermal stress was higher for d1 than d2. PMID: 24631925
- these data suggest that Adrm1, a new Atp6v0d2-interacting protein, plays an important role in osteoclast differentiation, and in particular the fusion of preosteoclasts. PMID: 19818731
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蛋白家族:V-ATPase V0D/AC39 subunit family
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組織特異性:Kidney, osteoclast and lung.
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數(shù)據(jù)庫(kù)鏈接:
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