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EEF2KMT Antibody

  • 中文名稱(chēng)：
    EEF2KMT兔多克隆抗體
  • 貨號(hào)：
    CSB-PA853420LA01HU
  • 規(guī)格：
    ￥880
  • 圖片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate(20μg), HepG2 whole cell lysate(20μg), 293Twhole cell lysate(20μg), THP-1 whole cell lysate(20μg), MCF7 whole cell lysate(20μg), A431 whole cell lysate(20μg)
      All lanes: EEF2KMT antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/20000 dilution
      Predicted band size: 37, 34 kDa
      Observed band size: 37 kDa
      Exposure time: 60s
    • IHC image of CSB-PA853420LA01HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
    • IHC image of CSB-PA853420LA01HU diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品名稱(chēng)：
    Rabbit anti-Homo sapiens (Human) EEF2KMT Polyclonal antibody
  • Uniprot No.：
  • 基因名：
    EEF2KMT
  • 別名：
    EEF2KMT antibody; FAM86A antibody; SB153Protein-lysine N-methyltransferase EEF2KMT antibody; EC 2.1.1.- antibody; eEF2-lysine methyltransferase antibody; eEF2-KMT antibody
  • 宿主：
    Rabbit
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    Synthesized peptide derived from human FA86A
  • 免疫原種屬：
    Homo sapiens (Human)
  • 克隆類(lèi)型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    Antigen affinity purification
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IHC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:3000
    IHC 1:100-1:300
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Catalyzes the trimethylation of eukaryotic elongation factor 2 (EEF2) on 'Lys-525'.
  • 基因功能參考文獻(xiàn)：
    1. Previously uncharacterized lysine-specific methyltransferase FAM86A and Yjr129c introduce a functionally important lysine methylation in eEF2. PMID: 25231979
  • 亞細(xì)胞定位：
    Cytoplasm.
  • 蛋白家族：
    Class I-like SAM-binding methyltransferase superfamily, EEF2KMT family
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 32221

    OMIM: 615263

    KEGG: hsa:196483

    STRING: 9606.ENSP00000398502

    UniGene: Hs.406461