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GPR75 Antibody

  • 中文名稱：
    GPR75兔多克隆抗體
  • 貨號：
    CSB-PA009829LA01HU
  • 規(guī)格：
    ￥440
  • 圖片：
    • Western Blot
      Positive WB detected in: HepG2 whole cell lysate(30μg), Hela whole cell lysate(30μg), SY5Y whole cell lysate(30μg)
      All lanes: GPR75 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/20000 dilution
      Predicted band size: 60 kDa
      Observed band size: 60 kDa
      Exposure time: 120s
    • Immunofluorescence staining of A549 cell with CSB-PA009829LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunofluorescence staining of A549 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    Initially, a rabbit was inoculated with recombinant human GPR75 protein (372-540aa) to generate the GPR75 polyclonal antibody. The resulting antibodies were extracted from the rabbit serum and then purified using protein G affinity chromatography. The purity level of the GPR75 polyclonal antibody was measured to exceed 95%, indicating minimal impurities.

    This GPR75 polyclonal antibody can recognize and bind to the GPR75 protein of human origin. Moreover, it has successfully validated its effectiveness in a range of experimental settings, including ELISA, WB, IHC, and IF, enabling the detection of levels, determination of presence and size, and analysis of the distribution of GPR75 protein.

  • 產(chǎn)品名稱：
    Rabbit anti-Homo sapiens (Human) GPR75 Polyclonal antibody
  • Uniprot No.：
  • 基因名：
  • 別名：
    G protein coupled receptor 75 antibody; GPR chr2 antibody; Gpr75 antibody; GPR75_HUMAN antibody; GPRchr2 antibody; OTTHUMP00000159608 antibody; Probable G protein coupled receptor 75 antibody; Probable G-protein coupled receptor 75 antibody; WI 31133 antibody; WI31133 antibody
  • 宿主：
    Rabbit
  • 反應種屬：
    Human
  • 免疫原：
    Recombinant Human Probable G-protein coupled receptor 75 protein (372-540AA)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated

    本頁面中的產(chǎn)品，GPR75 Antibody (CSB-PA009829LA01HU)，的標記方式是Non-conjugated。對于GPR75 Antibody，我們還提供其他標記。見下表：

    可提供標記
    標記方式 貨號 產(chǎn)品名稱 應用
    HRP CSB-PA009829LB01HU GPR75 Antibody, HRP conjugated ELISA
    FITC CSB-PA009829LC01HU GPR75 Antibody, FITC conjugated
    Biotin CSB-PA009829LD01HU GPR75 Antibody, Biotin conjugated ELISA
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    >95%, Protein G purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IF
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IF 1:20-1:100
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

  • 功能：
    G protein-coupled receptor that is activated by the chemokine CCL5/RANTES. Probably coupled to heterotrimeric Gq proteins, it stimulates inositol trisphosphate production and calcium mobilization upon activation. Together with CCL5/RANTES, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. CCL5/RANTES may also regulate insulin secretion by pancreatic islet cells through activation of this receptor.
  • 亞細胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    G-protein coupled receptor 1 family
  • 組織特異性：
    Expressed at high levels in brain and spinal cord and at detectable levels in retinal pigment epithelium. In situ hybridization of adult eye sections localized transcripts only to the perivascular cells, surrounding retinal arterioles, in the ganglion cel
  • 數(shù)據(jù)庫鏈接：

    HGNC: 4526

    OMIM: 606704

    KEGG: hsa:10936

    STRING: 9606.ENSP00000378195

    UniGene: Hs.40763