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HLA-C Antibody

  • 中文名稱(chēng)：
    HLA-C兔多克隆抗體
  • 貨號(hào)：
    CSB-PA15099A0Rb
  • 規(guī)格：
    ￥440
  • 圖片：
    • IHC image of CSB-PA15099A0Rb diluted at 1:400 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
    • Immunofluorescence staining of Hela cell with CSB-PA15099A0Rb at 1:80, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, A431 whole cell lysate
      All lanes: HLA-C antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 41 kDa
      Observed band size: 41 kDa
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    HLA-C polyclonal antibody CSB-PA15099A0Rb was produced in the rabbit immunized by using the Recombinant Human HLA-C protein (25-308AA) as the immunogen. The target protein HLA-C belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain. Class I molecules play a critical role in the immune system by presenting peptides derived from endoplasmic reticulum lumen. Diseases associated with HLA-C include Psoriasis 1 and Human Immunodeficiency Virus Type 1.
    This Rabbit anti-Homo sapiens (Human) HLA-C Polyclonal antibody was tested in the ELISA, WB, IHC and IF applications. The non-conjugated IgG got purified by protein G and reached up to 95% in purity. It only reacts with the HLA-C proteins of human-origin and may be used to detect the endogenous levels of HLA-C protein.
  • Uniprot No.：
  • 宿主：
    Rabbit
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    Recombinant Human HLA-C protein (25-308AA)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated

    本頁(yè)面中的產(chǎn)品，HLA-C Antibody (CSB-PA15099A0Rb)，的標(biāo)記方式是Non-conjugated。對(duì)于HLA-C Antibody，我們還提供其他標(biāo)記。見(jiàn)下表：

    可提供標(biāo)記
    標(biāo)記方式 貨號(hào) 產(chǎn)品名稱(chēng) 應(yīng)用
    HRP CSB-PA15099B0Rb HLA-C Antibody, HRP conjugated ELISA
    FITC CSB-PA15099C0Rb HLA-C Antibody, FITC conjugated
    Biotin CSB-PA15099D0Rb HLA-C Antibody, Biotin conjugated ELISA
  • 克隆類(lèi)型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    >95%, Protein G purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IHC, IF
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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