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POB3 Antibody

Rare Species
  • 中文名稱：
    POB3 Antibody
  • 貨號：
    CSB-PA279344XA01SVG
  • 規(guī)格：
    ￥880
  • 圖片：
    • Western Blot
      Positive WB detected in: recombinant protein
      All lanes:POB3 Antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/20000 dilution
      Predicted band size: 37 kDa
      Observed band size: 44 kDa
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品名稱：
    Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) POB3 Polyclonal antibody
  • Uniprot No.：
  • 基因名：
    POB3
  • 別名：
    FACT complex subunit POB3 (Facilitates chromatin transcription complex subunit POB3) POB3 YML069W
  • 宿主：
    Rabbit
  • 反應(yīng)種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 免疫原：
    Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) POB3 protein (289-552aa)
  • 免疫原種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    Antigen Affinity Purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

  • 功能：
    Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. Transcription elongation is promoted by the repression of transcription initiation from cryptic sites. Also acts in establishing transcription initiation complexes and promotes SPT15/TBP-binding to a TATA box. Together with replication factor-A protein (RPA), FACT may play a role in nucleosome deposition during DNA replication.
  • 基因功能參考文獻(xiàn)：
    1. FACT remodels the tetranucleosomal unit of chromatin fibers for gene transcription. PMID: 27666592
    2. FACT binding results in dramatic ATP-independent, symmetrical and reversible DNA uncoiling that affects at least 70% of the DNA within a nucleosome. Nucleosome unfolding by yFACT (Spt16-Pob3 with Nhp6) is extensive and reversible. PMID: 27820806
    3. Pob3-Q308K caused inefficient dissociation of FACT from nucleosomes. PMID: 23833181
    4. mutation of histone H3 lysine 56 decreases the association of histones with Pob3p PMID: 21656278
  • 亞細(xì)胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    SSRP1 family
  • 數(shù)據(jù)庫鏈接：

    KEGG: sce:YML069W

    STRING: 4932.YML069W