PRDM11 Antibody
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中文名稱:PRDM11兔多克隆抗體
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貨號:CSB-PA882077LA01HU
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規(guī)格:¥440
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圖片:
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Western Blot
Positive WB detected in: A549 whole cell lysate
All lanes: PRDM11 antibody at 5.6µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 58, 54 kDa
Observed band size: 58 kDa -
IHC image of CSB-PA882077LA01HU diluted at 1:600 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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IHC image of CSB-PA882077LA01HU diluted at 1:600 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunofluorescence staining of Hela cells with CSB-PA882077LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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其他:
產(chǎn)品詳情
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產(chǎn)品名稱:Rabbit anti-Homo sapiens (Human) PRDM11 Polyclonal antibody
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Uniprot No.:
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基因名:PRDM11
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別名:PRDM11 antibody; PFM8 antibody; PR domain-containing protein 11 antibody; EC 2.1.1.- antibody
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宿主:Rabbit
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反應(yīng)種屬:Human
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免疫原:Recombinant Human PR domain-containing protein 11 protein (63-215AA)
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免疫原種屬:Homo sapiens (Human)
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標(biāo)記方式:Non-conjugated
本頁面中的產(chǎn)品,PRDM11 Antibody (CSB-PA882077LA01HU),的標(biāo)記方式是Non-conjugated。對于PRDM11 Antibody,我們還提供其他標(biāo)記。見下表:
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克隆類型:Polyclonal
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抗體亞型:IgG
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純化方式:>95%, Protein G purified
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濃度:It differs from different batches. Please contact us to confirm it.
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保存緩沖液:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 -
產(chǎn)品提供形式:Liquid
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應(yīng)用范圍:ELISA, WB, IHC, IF
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推薦稀釋比:
Application Recommended Dilution WB 1:500-1:5000 IHC 1:500-1:1000 IF 1:200-1:500 -
Protocols:
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儲(chǔ)存條件:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期:Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途:For Research Use Only. Not for use in diagnostic or therapeutic procedures.
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能:May be involved in transcription regulation.
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基因功能參考文獻(xiàn):
- We characterize PRDM11 as a putative novel tumor suppressor that controls the expression of key oncogenes, and we add new mechanistic insight into B-cell lymphomagenesis. PMID: 25499759
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亞細(xì)胞定位:Nucleus. Cytoplasm.
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蛋白家族:Class V-like SAM-binding methyltransferase superfamily
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組織特異性:Highly expressed in lung, including bronchial epithelial cells and airway smooth muscle cells, as well as in peripheral blood mononuclear cells. In tonsils, expressed in B-cell types, including naive B-cells, centroblasts, centrocytes and memory B-cells (
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