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RALY Antibody

  • 中文名稱：
    RALY兔多克隆抗體
  • 貨號：
    CSB-PA892332LA01HU
  • 規(guī)格：
    ￥440
  • 圖片：
    • Western Blot
      Positive WB detected in: A549 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate
      All lanes: RALY antibody at 5.7µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 33, 31 kDa
      Observed band size: 33 kDa
    • IHC image of CSB-PA892332LA01HU diluted at 1:500 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA892332LA01HU diluted at 1:500 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of Hela cells with CSB-PA892332LA01HU at 1:167, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品名稱：
    Rabbit anti-Homo sapiens (Human) RALY Polyclonal antibody
  • Uniprot No.：
  • 基因名：
    RALY
  • 別名：
    Autoantigen p542 antibody; Heterogeneous nuclear ribonucleoprotein C-like 2 antibody; hnRNP associated with lethal yellow protein homolog antibody; hnRNP core protein C-like 2 antibody; MGC117312 antibody; P542 antibody; RALY antibody; RALY_HUMAN antibody; RNA binding protein (autoantigenic hnRNP associated with lethal yellow) antibody; RNA binding protein (autoantigenic) antibody; RNA binding protein autoantigenic (hnRNP associated with lethal yellow homolog (mouse)) antibody; RNA-binding protein Raly antibody
  • 宿主：
    Rabbit
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    Recombinant Human RNA-binding protein Raly protein (254-306AA)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated

    本頁面中的產(chǎn)品，RALY Antibody (CSB-PA892332LA01HU)，的標(biāo)記方式是Non-conjugated。對于RALY Antibody，我們還提供其他標(biāo)記。見下表：

    可提供標(biāo)記
    標(biāo)記方式 貨號 產(chǎn)品名稱 應(yīng)用
    HRP CSB-PA892332LB01HU RALY Antibody, HRP conjugated ELISA
    FITC CSB-PA892332LC01HU RALY Antibody, FITC conjugated
    Biotin CSB-PA892332LD01HU RALY Antibody, Biotin conjugated ELISA
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    >95%, Protein G purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IHC, IF
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:500-1:1000
    IF 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    RNA-binding protein that acts as a transcriptional cofactor for cholesterol biosynthetic genes in the liver. Binds the lipid-responsive non-coding RNA LeXis and is required for LeXis-mediated effect on cholesterogenesis. May be a heterogeneous nuclear ribonucleoprotein (hnRNP).
  • 基因功能參考文獻(xiàn)：
    1. hnRNP RALY regulates PMID: 28972179
    2. RALY promotes Protein Arginine Methyltransferase 1 alternatively spliced isoform v2 relative expression and metastatic potential in breast cancer cells PMID: 28733251
    3. RALY is a poly-U binding protein and as a regulator of H1FX and ANXA1 in mammalian cells. PMID: 28379492
    4. People with lower level of RALYL expression had a poorer survival rate. PMID: 22994768
  • 亞細(xì)胞定位：
    Nucleus.
  • 蛋白家族：
    RRM HNRPC family, RALY subfamily
  • 組織特異性：
    Expressed in heart, brain, lung, liver, skeletal muscle, kidney and pancreas. Weakly expressed in placenta.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 15921

    OMIM: 614663

    KEGG: hsa:22913

    STRING: 9606.ENSP00000246194

    UniGene: Hs.136947