RHBDD3 Antibody
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中文名稱:RHBDD3兔多克隆抗體
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貨號(hào):CSB-PA27742A0Rb
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規(guī)格:¥440
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圖片:
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Western Blot
Positive WB detected in: PC-3 whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate
All lanes: RHBDD3 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa -
IHC image of CSB-PA27742A0Rb diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunofluorescence staining of U251 cells with CSB-PA27742A0Rb at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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其他:
產(chǎn)品詳情
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產(chǎn)品名稱:Rabbit anti-Homo sapiens (Human) RHBDD3 Polyclonal antibody
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Uniprot No.:
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基因名:RHBDD3
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別名:C22orf3 antibody; HS984G1A antibody; Pituitary tumor apoptosis antibody; PTAG antibody; RHBD3_HUMAN antibody; RHBDD3 antibody; Rhomboid domain containing 3 antibody; Rhomboid domain-containing protein 3 antibody
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宿主:Rabbit
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反應(yīng)種屬:Human
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免疫原:Peptide sequence from Human Rhomboid domain-containing protein 3 protein (304-323AA)
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免疫原種屬:Homo sapiens (Human)
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標(biāo)記方式:Non-conjugated
本頁(yè)面中的產(chǎn)品,RHBDD3 Antibody (CSB-PA27742A0Rb),的標(biāo)記方式是Non-conjugated。對(duì)于RHBDD3 Antibody,我們還提供其他標(biāo)記。見下表:
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克隆類型:Polyclonal
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抗體亞型:IgG
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純化方式:Antigen Affinity Purified
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濃度:It differs from different batches. Please contact us to confirm it.
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保存緩沖液:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 -
產(chǎn)品提供形式:Liquid
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應(yīng)用范圍:ELISA, WB, IHC, IF
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推薦稀釋比:
Application Recommended Dilution WB 1:500-1:2000 IHC 1:20-1:200 IF 1:50-1:200 -
Protocols:
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儲(chǔ)存條件:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期:Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途:For Research Use Only. Not for use in diagnostic or therapeutic procedures.
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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基因功能參考文獻(xiàn):
- RHBDD3 is a target gene of the BACH1 transcription factor according to ChIP-seq analysis in HEK 293 cells. PMID: 21555518
- isolation of a novel differentially methylated chromosome 22 CpG island-associated gene PTAG PMID: 15105437
- Loss of PTAG contributes to a blunted apoptotic response and is likely to predispose cells toward malignant transformation and resistance to chemotherapeutic interventions. PMID: 17117413
- the EWSR1 promoter functions in a bidirectional manner, thereby regulating also RHBDD3 PMID: 19212622
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亞細(xì)胞定位:Membrane; Multi-pass membrane protein.
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數(shù)據(jù)庫(kù)鏈接:
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