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  4. TMEM176B Antibody
  • 中文名稱：
    TMEM176B兔多克隆抗體
  • 貨號：
    CSB-PA023758LA01HU
  • 規(guī)格：
    ￥440
  • 圖片：
    • Western Blot
      Positive WB detected in: THP-1 whole cell lysate(30μg), SY5Y whole cell lysate(30μg), Hela whole cell lysate(30μg), K562 whole cell lysate(30μg)
      All lanes: TMEM176B antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/20000 dilution
      Predicted band size: 30,26 kDa
      Observed band size: 30 kDa
      Exposure time：120s
    • Immunofluorescence staining of Hela cell with CSB-PA023758LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    To produce the TMEM176B antibody, a rabbit was first immunized with the recombinant human transmembrane protein 176B (1-64aa). Following this, the rabbit serum was collected and the antibody was isolated and purified through protein G affinity chromatography.

    The resulting antibody showed high purity levels of up to 95% and displayed reactivity solely with human TMEM176B protein. Quality verification tests were conducted on the rabbit anti-human TMEM176B polyclonal antibody, including ELISA, WB, IHC, and IF, confirming its suitability for use in various applications.

  • 產(chǎn)品名稱：
    Rabbit anti-Homo sapiens (Human) TMEM176B Polyclonal antibody
  • Uniprot No.：
  • 基因名：
    TMEM176B
  • 別名：
    TMEM176B; LR8; Transmembrane protein 176B; Protein LR8
  • 宿主：
    Rabbit
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    Synthesized peptide derived from human TMEM176B
  • 免疫原種屬：
    Homo sapiens (Human)
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    Antigen affinity purification
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IF
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IF 1:10-1:100
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    May play a role in the process of maturation of dendritic cells. Required for the development of cerebellar granule cells.
  • 基因功能參考文獻(xiàn)：
    1. Study characterized Tmem176a and b expression in RORgamma t+ lymphocytes at transcriptional and protein levels and present evidence that both genes exert a redundant ion channel function related to a colocalisation in close proximity to the Golgi apparatus. PMID: 27009467
    2. human TMEM176A and 176B protein levels are significantly elevated in lymphoma, but not in normal tissues PMID: 22244448
  • 亞細(xì)胞定位：
    Nucleus membrane; Multi-pass membrane protein.
  • 蛋白家族：
    TMEM176 family
  • 組織特異性：
    Expressed in lung and dermal fibroblasts.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 29596

    OMIM: 610385

    KEGG: hsa:28959

    STRING: 9606.ENSP00000318409

    UniGene: Hs.647090