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  4. VACWR106 Antibody
  • 中文名稱(chēng)：
    VACWR106兔多克隆抗體
  • 貨號(hào)：
    CSB-PA366132XA01VAI
  • 規(guī)格：
    ￥880
  • 圖片：
    • Western Blot
      Positive WB detected in: recombinant protein
      All lanes:VACWR106 Antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 40.2 kDa
      Observed band size: 41 kDa
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品名稱(chēng)：
    Rabbit anti-Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR)) VACWR106 Antibody Polyclonal antibody
  • Uniprot No.：
  • 基因名：
    VACWR106 Antibody
  • 別名：
    mRNA-capping enzyme catalytic subunit (Virus termination factor large subunit) (VTF large subunit) (mRNA-capping enzyme 97 kDa subunit) (mRNA-capping enzyme D1 subunit) (mRNA-capping enzyme large subunit) [Includes: Polynucleotide 5'-triphosphatase (EC 3.1.3.33) (mRNA 5'-triphosphatase) (TPase); mRNA guanylyltransferase (EC 2.7.7.50) (GTP--RNA guanylyltransferase) (GTase); mRNA (guanine-N(7)-)-methyltransferase (EC 2.1.1.56) (mRNA cap methyltransferase)] VACWR106 D1R
  • 宿主：
    Rabbit
  • 反應(yīng)種屬：
    Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
  • 免疫原：
    Recombinant Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR)) VACWR106 protein (516-844aa)
  • 免疫原種屬：
    Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類(lèi)型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    >95%, Protein G purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Catalytic subunit of the mRNA capping enzyme which catalyzes three enzymatic reactions: the 5' triphosphate end of the pre-mRNA is hydrolyzed to a diphosphate by RNA 5' triphosphatase; the diphosphate RNA end is capped with GMP by RNA guanylyltransferase and the GpppN cap is methylated by RNA (guanine-N7) methyltransferase. Heterodimeric mRNA capping enzyme catalyzes the linkage of a N7-methyl-guanosine moiety to the first transcribed nucleotide (cap 0 structure), whereas the polymerase associated VP39 is responsible for a second methylation at the 2'-O position of the ribose (cap 1 structure).; The heterodimeric enzyme is also involved in early viral gene transcription termination and intermediate viral gene transcription initiation. Early gene transcription termination requires the termination factor VTF, the DNA-dependent ATPase NPH-I and the Rap94 subunit of the viral RNA polymerase, as well as the presence of a specific termination motif. Binds, together with RAP94, to the termination motif 5'-UUUUUNU-3' in the nascent early mRNA.
  • 基因功能參考文獻(xiàn)：
    1. VTF provides the connection between the termination signal in the RNA transcript and viral RNA polymerase to initiate transcription termination. PMID: 26280468
    2. Crystal structure of vaccinia virus mRNA capping enzyme PMID: 24607143
    3. the results presented in this study provide the first in vivo evidence that the vaccinia virus mRNA capping enzyme plays a role in early gene transcription termination and intermediate gene transcription. PMID: 18295814
  • 亞細(xì)胞定位：
    Virion. Note=All the enzymes and other proteins required to synthesize early mRNAs are packaged within the virion core along with the DNA genome.
  • 蛋白家族：
    DsDNA virus mRNA guanylyltransferase family; Class I-like SAM-binding methyltransferase superfamily, mRNA cap 0 methyltransferase family
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: vg:3707562