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  4. ligA Antibody
  • 中文名稱：
    ligA兔多克隆抗體
  • 貨號(hào)：
    CSB-PA322745HA01ENV
  • 規(guī)格：
    ￥440
  • 圖片：
    • Western blot
      All lanes: DNA ligase antibody at 2μg/ml + DH5a whole cell lysate
      Secondary
      Goat polyclonal to rabbit IgG at 1/10000 dilution
      Predicted band size: 74 kDa
      Observed band size: 74 kDa
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品名稱：
    Rabbit anti-Escherichia coli ligA Polyclonal antibody
  • Uniprot No.：
  • 基因名：
    ligA
  • 別名：
    ligA antibody; dnaL antibody; lig antibody; lop antibody; pdeC antibody; b2411 antibody; JW2403 antibody; DNA ligase antibody; EC 6.5.1.2 antibody; Polydeoxyribonucleotide synthase [NAD(+)] antibody
  • 宿主：
    Rabbit
  • 反應(yīng)種屬：
    Escherichia coli
  • 免疫原：
    Recombinant Escherichia coli DNA ligase protein (1-671AA)
  • 免疫原種屬：
    Escherichia coli
  • 標(biāo)記方式：
    Non-conjugated

    本頁(yè)面中的產(chǎn)品，ligA Antibody (CSB-PA322745HA01ENV)，的標(biāo)記方式是Non-conjugated。對(duì)于ligA Antibody，我們還提供其他標(biāo)記。見(jiàn)下表：

    可提供標(biāo)記
    標(biāo)記方式 貨號(hào) 產(chǎn)品名稱 應(yīng)用
    HRP CSB-PA322745HB01ENV ligA Antibody, HRP conjugated ELISA
    FITC CSB-PA322745HC01ENV ligA Antibody, FITC conjugated
    Biotin CSB-PA322745HD01ENV ligA Antibody, Biotin conjugated ELISA
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    >95%, Protein G purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:5000
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.
  • 基因功能參考文獻(xiàn)：
    1. Kinetic mechanism and fidelity of nick sealing by Escherichia coli LigA has been described. PMID: 26857547
    2. Here the authors show that replication without ligase kills after a short resistance period. PMID: 22582878
    3. temperature sensitive mutant characterized PMID: 15583169
    4. analysis of the nucleotidyltransferase domain of Escherichia coli NAD+-dependent DNA ligase (LigA) PMID: 15671015
    5. The deletion of the BRCT domain from LigA reduced in vitro ligation activity by 3-fold and also reduced DNA binding. PMID: 15848142
    6. 2.3 A crystal structure of LigA bound to an adenylylated nick, which captures LigA in a state poised for strand closure and reveals the basis for nick recognition PMID: 17466627
    7. structure-function analysis of the OB, HhH & BRCT domains of LigA; study found that the BRCT domain is required in its entirety for effective nick sealing and AMP-dependent supercoil relaxation PMID: 18515356

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  • 蛋白家族：
    NAD-dependent DNA ligase family, LigA subfamily
  • 數(shù)據(jù)庫(kù)鏈接：