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rho Antibody

Rare Species
  • 中文名稱：
    rho兔多克隆抗體
  • 貨號：
    CSB-PA16627A0Rb
  • 規(guī)格：
    ￥440
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品名稱：
    Rabbit anti-Escherichia coli rho Polyclonal antibody
  • Uniprot No.：
  • 基因名：
  • 別名：
    rho antibody; nitA antibody; psuA antibody; rnsC antibody; sbaA antibody; tsu antibody; b3783 antibody; JW3756 antibody; Transcription termination factor Rho antibody; EC 3.6.4.- antibody; ATP-dependent helicase Rho antibody
  • 宿主：
    Rabbit
  • 反應(yīng)種屬：
    Escherichia coli
  • 免疫原：
    Recombinant Escherichia coli Transcription termination factor Rho protein (1-419AA)
  • 免疫原種屬：
    Escherichia coli
  • 標(biāo)記方式：
    Non-conjugated

    本頁面中的產(chǎn)品，rho Antibody (CSB-PA16627A0Rb)，的標(biāo)記方式是Non-conjugated。對于rho Antibody，我們還提供其他標(biāo)記。見下表：

    可提供標(biāo)記
    標(biāo)記方式 貨號 產(chǎn)品名稱 應(yīng)用
    HRP CSB-PA16627B0Rb rho Antibody, HRP conjugated ELISA
    FITC CSB-PA16627C0Rb rho Antibody, FITC conjugated
    Biotin CSB-PA16627D0Rb rho Antibody, Biotin conjugated ELISA
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    >95%, Protein G purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

  • 功能：
    Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template. RNA-dependent NTPase which utilizes all four ribonucleoside triphosphates as substrates.
  • 基因功能參考文獻：
    1. Our findings further show that the RNA sequence specificity used for guiding Rho-dependent termination derives in part from an intrinsic ability of the motor to couple the recognition of pyrimidine patterns in nascent transcripts to RNA loading and activity. PMID: 27821776
    2. results, together with existing data, support a model in which the connector segment plays a hitherto overlooked role in the regulation of Rho-dependent termination PMID: 28559482
    3. Translational control and Rho-dependent transcription termination are intimately linked in riboswitch regulation. PMID: 28520932
    4. NusG acts as both a positive and negative regulator of Rho in the course of the bacterial transcription termination. (Review) PMID: 27023849
    5. Finally, identification of the NusG binding sites on the Rho hexamer led us to conclude that the former exerts its effect allosterically. PMID: 27605667
    6. Rho inhibition leads to RNA polymerase readthrough, which in principle could displace H-NS from the DNA, thus leading to transcriptional derepression of H-NS-silenced genes. PMID: 24499790
    7. Rho binds C-rich unstructured nascent RNA (high C/G ratio) prior to its ATP-dependent dissociation of transcription complexes PMID: 23207917
    8. Tthe in vivo Rho-dependent termination process is kinetically controlled. PMID: 22442304
    9. Here the authors provide direct evidence that the beta-sheet bundle of the C-terminal domain of NusG (NusG-CTD) has the binding determinants for Rho. PMID: 21040729
    10. The authors mutated E211, R366, R212, and D265, and characterized the resulting proteins for oligomerization, ligand binding and RNA-dependent ATP hydrolysis that support the existing model of ATP hydrolysis. PMID: 20950626
    11. Global hydrogen-deuterium exchange indicate net mass differences of about 15 Da after 1 h of exchange in the presence--versus in the absence--of the ligand MgATP or the RNA poly(C). PMID: 20708016
    12. Results indicate that all three Rho catalytic sites must be filled with substrate to achieve the enhanced catalytic rate, both in pre-steady-state and in steady-state hydrolysis. PMID: 15703177
    13. Results reinforce the importance of catalytic cooperativity in normal Rho function and suggest that several protein conformations exist along the catalytic pathway. PMID: 15703178
    14. findings show that transcription termination of fimE is Rho dependent and is suppressed in a rho mutant or by bicyclomycin treatment when fimE mRNA is expressed by the fimE gene, either from a recombinant plasmid or in its native chromosomal location PMID: 16321930
    15. mutant forms of Rho were defective in transcriptional termination, suggesting that those residues play an important role in the activation of Rho by bound RNA PMID: 16908525
    16. interactions in the primary RNA binding domain and in the Q-loop are mandatory for RNA release to occur and propose that the interactions in the primary RNA binding modulate most of the other functions of Rho allosterically PMID: 17599352
    17. results reveal Rho factor as a global regulator of gene expression under normal growth conditions; it serves role of maintaining transcriptional boundaries; Rho termination, supported by NusA & NusG, is required to suppress toxic activity of foreign genes PMID: 18487194
    18. ADP but not P(i) dissociation contributes to rate limitation for Escherichia coli Rho PMID: 19837672
    19. These data show that Rho forms uneven productive interactions with the track nucleotides and disrupts RNA-DNA duplexes in a succession of large (approximately 7-nucleotide-long) discrete steps triggered by 2'-hydroxyl activation events. PMID: 19915588

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  • 蛋白家族：
    Rho family
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