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  4. ACVR2A&ACVR2B Recombinant Monoclonal Antibody

ACVR2A&ACVR2B Recombinant Monoclonal Antibody

  • 中文名稱：
    ACVR2A&ACVR2B Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA623829MA1HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • The Binding Activity of Human ACVR2A with Anti-ACVR2A&ACVR2B recombinant antibody
      Activity: Measured by its binding ability in a functional ELISA. Immobilized Human ACVR2A（CSB-MP001260HU1）at 2 μg/mL can bind Anti-ACVR2A&ACVR2B recombinant antibody . The EC50 is 3.848-4.375 ng/mL.
    • The Binding Activity of Mouse ACVR2A with Anti-ACVR2A&ACVR2B recombinant antibody
      Activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse ACVR2A（CSB-MP001260MO1） at 2 μg/mL can bind Anti-ACVR2A&ACVR2B recombinant antibody. The EC50 is 1.562-1.966 ng/mL.
    • The Binding Activity of Mouse Acvr2b with Anti-ACVR2A&ACVR2B recombinant antibody
      Activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse Acvr2b（CSB-MP001261MO1） at 2 μg/mL can bind Anti-ACVR2A&ACVR2B recombinant antibody. The EC50 is 3.560-4.235 ng/mL.
    • The Binding Activity of Human ACVR2B with Anti-ACVR2A&ACVR2B recombinant antibody
      Activity: Measured by its binding ability in a functional ELISA. Immobilized Human ACVR2B（CSB-MP623829HU） at 2 μg/mL can bind Anti-ACVR2A&ACVR2B recombinant antibody. The EC50 is 2.983-3.438 ng/mL.
    • IHC image of CSB-RA623829MA1HU diluted at 1:200 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA623829MA1HU diluted at 1:200 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of SH-SY5Y cell with CSB-RA623829MA1HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Human IgG(H+L).
    • The purity of ACVR2A&ACVR2B was greater than 95% as determined by SEC-HPLC
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    ACVR2B重組單克隆抗體（CUSABIO貨號：CSB-RA623829MA1HU）是針對激活素受體IIB型（ACVR2B）靶點開發(fā)的高特異性科研工具。ACVR2B作為TGF-β超家族的重要成員，通過介導激活素、肌抑素等配體信號傳導，在肌肉生長調(diào)控、代謝平衡及組織修復中發(fā)揮關(guān)鍵作用。本抗體經(jīng)多種實驗平臺嚴格驗證，適用于ELISA、免疫組織化學（IHC）和免疫熒光（IF）檢測，其中IHC和IF實驗推薦使用1:50-1:200的稀釋范圍，可在多種樣本類型中實現(xiàn)清晰的靶標定位與信號特異性。實驗數(shù)據(jù)顯示，該抗體在石蠟包埋組織切片中能有效識別天然構(gòu)象的ACVR2B蛋白，同時在細胞免疫熒光實驗中展現(xiàn)出優(yōu)異的亞細胞定位能力，背景干擾低且批次穩(wěn)定性優(yōu)異。適用于研究肌肉發(fā)育相關(guān)機制、代謝性疾病模型、骨骼肌再生及纖維化病理過程等科研領(lǐng)域，為探究ACVR2B在細胞信號通路中的功能及其與相關(guān)疾病的關(guān)聯(lián)提供可靠檢測方案。
  • Uniprot No.：
  • 基因名：
    ACVR2A&ACVR2B
  • 別名：
    Activin A receptor type IIA antibody; Activin receptor type 2A antibody; Activin receptor type IIA antibody; Activin receptor type-2A antibody; ACTR 2 antibody; ACTR IIA antibody; ACTR-IIA antibody; ACTR2 antibody; ACTRII antibody; ACTRIIA antibody; Acvr 2 antibody; Acvr 2A antibody; Acvr2 antibody; ACVR2A antibody; AVR2A_HUMAN antibody; OTTHUMP00000197918 antibody; Activin A receptor type IIB antibody; Activin receptor type 2B antibody; Activin receptor type IIB antibody; Activin receptor type-2B antibody; ActR IIB antibody; ACTR-IIB antibody; ActRIIB antibody; ACVR 2B antibody; ACVR2B antibody; AVR2B_HUMAN antibody; HTX4 antibody; MGC116908 antibody
  • 反應(yīng)種屬：
    Human, Mouse
  • 免疫原：
    Recombinant Human ACVR2A&ACVR2B protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    hIgG1
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    7E5
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, IHC, IF
  • 推薦稀釋比：
    Application Recommended Dilution
    ELISA 1:5000-1:50000
    IHC 1:50-1:200
    IF 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價