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AKT1/2/3 Recombinant Monoclonal Antibody

  • 中文名稱：
    AKT1/2/3重組抗體
  • 貨號(hào)：
    CSB-RA272392A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: SH-SY5Y whole cell lysate
      All lanes: CD44 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 56 kDa
      Observed band size: 56 kDa
    • IHC image of CSB-RA272392A0HU diluted at 1:50 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.54% DAB.
    • Overlay Peak curve showing Jurkat cells stained with CSB-RA272392A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    AKT1/2/3 Recombinant Monoclonal Antibody（Code: CSB-RA272392A0HU）是一款經(jīng)多平臺(tái)驗(yàn)證的高特異性抗體，靶向AKT激酶家族成員（AKT1、AKT2、AKT3）。AKT蛋白在PI3K-AKT-mTOR信號(hào)通路中發(fā)揮核心作用，參與調(diào)控細(xì)胞增殖、存活和代謝等關(guān)鍵生物學(xué)過程，其異常激活與腫瘤發(fā)生、耐藥性及代謝性疾病密切相關(guān)。本抗體采用重組單克隆技術(shù)開發(fā)，可精準(zhǔn)識(shí)別內(nèi)源性AKT蛋白，經(jīng)嚴(yán)格驗(yàn)證適用于ELISA、Western Blot（推薦稀釋1:500-1:2000）、免疫組化（推薦稀釋1:50-1:200）和流式細(xì)胞術(shù)（推薦稀釋1:50-1:200）。實(shí)驗(yàn)數(shù)據(jù)顯示其在不同樣本類型（如細(xì)胞裂解物、石蠟包埋組織切片及懸浮細(xì)胞）中均能呈現(xiàn)清晰的檢測(cè)信號(hào)，Western Blot檢測(cè)可區(qū)分目標(biāo)蛋白的預(yù)期分子量，免疫組化染色定位準(zhǔn)確且背景低，流式分析靈敏度滿足細(xì)胞表面及胞內(nèi)標(biāo)記需求。該產(chǎn)品適用于癌癥機(jī)制研究、信號(hào)通路互作分析、藥物靶點(diǎn)篩選及代謝調(diào)控相關(guān)基礎(chǔ)科研，其高批次一致性為實(shí)驗(yàn)重復(fù)性提供可靠保障，助力研究者深入探索AKT蛋白在疾病模型中的功能與調(diào)控網(wǎng)絡(luò)。
  • Uniprot No.：
  • 基因名：
    AKT1/2/3
  • 別名：
    RAC-alpha serine/threonine-protein kinase (EC 2.7.11.1) (Protein kinase B) (PKB) (Protein kinase B alpha) (PKB alpha) (Proto-oncogene c-Akt) (RAC-PK-alpha), AKT1, PKB RAC, RAC-beta serine/threonine-protein kinase (EC 2.7.11.1) (Protein kinase Akt-2) (Protein kinase B beta) (PKB beta) (RAC-PK-beta), AKT2, RAC-gamma serine/threonine-protein kinase (EC 2.7.11.1) (Protein kinase Akt-3) (Protein kinase B gamma) (PKB gamma) (RAC-PK-gamma) (STK-2), AKT3, PKBG
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from Human AKT1/2/3 Antibody
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號(hào)：
    16E10
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IHC, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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