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  4. ANXA4 Recombinant Monoclonal Antibody

ANXA4 Recombinant Monoclonal Antibody

  • 中文名稱：
    ANXA4重組抗體
  • 貨號：
    CSB-RA001845MA1HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, MCF7 whole cell lysate, U251 whole cell lysate, PC-3 whole cell lysate, Mouse Liver tissue lysate
      All lanes: ANXA4 antibody at 1:500
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 36 kDa
      Observed band size: 36 kDa
    • Overlay Peak curve showing Hela cells stained with CSB-RA001845MA1HU (red line) at 1:800. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1μg/1*106cells) used under the same conditions. Acquisition of >10,017 events was performed.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    CSB-RA001845MA1HU ANXA4重組單克隆抗體是針對膜聯(lián)蛋白A4（ANXA4）靶點開發(fā)的高特異性科研試劑，已通過ELISA、Western Blot（WB）和流式細(xì)胞術(shù)（FC）等多種實驗驗證。該抗體在WB實驗中可識別天然及重組ANXA4蛋白，推薦使用稀釋度1:1000-1:5000，能清晰檢測目標(biāo)蛋白條帶；在FC應(yīng)用中（推薦稀釋度1:20-1:200）可有效標(biāo)記細(xì)胞表面ANXA4，呈現(xiàn)特異性熒光信號。ANXA4作為鈣離子依賴性磷脂結(jié)合蛋白，廣泛參與細(xì)胞膜轉(zhuǎn)運、胞吐作用及腫瘤微環(huán)境調(diào)控，其異常表達(dá)與多種腫瘤的轉(zhuǎn)移及耐藥性相關(guān)。本產(chǎn)品適用于體外研究ANXA4在腫瘤生物學(xué)中的功能機(jī)制、細(xì)胞黏附與信號轉(zhuǎn)導(dǎo)過程，以及探索其在藥物敏感性調(diào)控中的作用，為癌癥研究、細(xì)胞生物學(xué)及分子藥理學(xué)領(lǐng)域提供可靠工具。所有數(shù)據(jù)均經(jīng)嚴(yán)格質(zhì)控，批次穩(wěn)定性優(yōu)異，適用于基礎(chǔ)科研中的蛋白定位、表達(dá)分析及功能研究。
  • Uniprot No.：
  • 基因名：
    ANXA4
  • 別名：
    35 beta calcimedin antibody; 35-beta calcimedin antibody; AIV antibody; Annexin 4 antibody; Annexin A4 antibody; Annexin IV (placental anticoagulant protein II) antibody; Annexin IV antibody; Annexin IV placental anticoagulant protein II antibody; Annexin-4 antibody; AnnexinA4 antibody; AnnexinIV antibody; ANX 4 antibody; ANX A4 antibody; ANX4 antibody; ANXA 4 antibody; ANXA4 antibody; ANXA4 protein antibody; ANXA4_HUMAN antibody; Carbohydrate binding protein P33/P41 antibody; Carbohydrate-binding protein p33/p41 antibody; Chromobindin 4 antibody; Chromobindin-4 antibody; Chromobindin4 antibody; DKFZp686H02120 antibody; Endonexin I antibody; EndonexinI antibody; Lipocortin IV antibody; LipocortinIV antibody; MGC75105 antibody; OTTHUMP00000160052 antibody; OTTHUMP00000202207 antibody; P32.5 antibody; P33/41 antibody; PAP II antibody; PAP-II antibody; PAPII antibody; PIG 28 antibody; PIG28 antibody; Placental anticoagulant protein II antibody; PP4 X antibody; PP4-X antibody; PP4X antibody; Proliferation inducing gene 28 antibody; Proliferation inducing protein 28 antibody; Protein II antibody; Xanx-4 antibody; ZAP 36 antibody; ZAP36 antibody
  • 反應(yīng)種屬：
    Human, Mouse
  • 免疫原：
    Recombinant Human ANXA4 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Mouse IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    31D6
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    FC 1:20-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

  • 功能：
    Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis.
  • 基因功能參考文獻(xiàn)：
    1. ANXA4 expression was increased at both the mRNA and protein level in the drugresistant ovarian cancer cells, and ANXA4 contained a Lewis(y) structure. ANXA4 overexpression can abnormally activate signaling pathways and regulate the expression of a numbers of factors, forming a positive feedback loop to induce the chemoresistance of ovarian cancer cells. PMID: 30066907
    2. Annexin A4 binds to artificial membranes and generates curvature force initiated from free edges, whereas annexin A6 induces constriction force. PMID: 29158488
    3. Taken together, these data indicate that up-regulation of ANXA4 leads to activation of the NF-kappaB pathway and its target genes in a feedback regulatory mechanism via the p65 subunit, resulting in tumor growth in GBC. PMID: 27491820
    4. Upregulation and nuclear translocation of ANXA4 have been observed in the progression of colorectal cancer and ovarian serous carcinoma. Knockdown of ANXA4 attenuated migration in ovarian cancer and breast cancer cells. In contrast, knockdown of ANXA4 increased susceptibility to platinum in ovarian cancer and malignant mesothelioma cells. Review. PMID: 27100483
    5. Study identifies ANXA4 and FLNA as up-regulated in buccal squamous cell carcinoma arising from oral submucous fibrosis. PMID: 27485544
    6. High ANXA4 expression is associated with metastasis of hepatocellular carcinoma. PMID: 26779633
    7. annexin A4 can be regarded as an important molecular marker in triple-negative breast cancer prognosis. PMID: 27650619
    8. role of annexin A4 in cancer PMID: 26048190
    9. Data shows that CYTB and ANXA4 overexpression may be involved in carcinogenesis and histopathological differentiation of ovarian clear cell carcinoma and suggest they may serve as a potential diagnostic biomarkers. PMID: 25633807
    10. These findings indicate that the calcium-binding site in the ANXA4 repeat induces chemoresistance to the platinum-based drug by elevating the intracellular chloride concentration. PMID: 25277200
    11. Fhit delocalizes annexin A4 from plasma membrane to cytosol and sensitizes lung cancer cells to paclitaxel. PMID: 24223161
    12. enhanced expression of Anx A4 confers platinum resistance by promoting efflux of platinum drugs via ATP7A. PMID: 24150977
    13. This study showed that overexpression and nuclear localization of annexin A4 are related to chemoresistance and poor survival in patients with serous papillary ovarian carcinomas. PMID: 23290009
    14. suggest that ANXA4 triggers a signaling cascade, leading to increased epithelial cell proliferation, ultimately promoting carcinogenesis PMID: 22970268
    15. Lower expression of annexin A4 during window of implantation in infertile patients with endometriosis might be associated with the decrease of endometrial receptivity. PMID: 22883517
    16. Eight ANXA4 polymorphisms are significantly associated with the risk of aspirin-exacerbated respiratory disease. PMID: 22847161
    17. that H3K4 tri- and dimethylation play an important role and that JARID1A is the histone-demethylating enzyme responsible for removal of this mark PMID: 21348943
    18. In an Alzheimer model, transgenic mice overexpressing mutant human amyloid precursor protein (APP) show a significant increase of annexin A5 in the brain cortex but not in other organs, including liver, kidney, lung, and intestine. PMID: 20648654
    19. Annexin A4 differentially modulates the NF-kappaB signaling pathway, depending on its interactions with p50 and the intracellular Ca(2+) ion level. PMID: 20237821
    20. The overexpression of Annexin IV may be an ovarian CCC-specific molecular marker. PMID: 19955935
    21. annexin IV plays an important role in the morphological diversification and dissemination of the clear cell renal cell carcinoma PMID: 15145526
    22. This work provides mechanistic insight into how annexin A4 may regulate plasma membrane protein function. PMID: 16687573
    23. ANXA4 transcription and translation are regulated by progesterone. ANXA4 may be important in regulating ion and water transport across the endometrial epithelium. PMID: 16954445
    24. There were no significant differences in plasma annexin IV levels between women with and without antiphospholipid antibodies PMID: 17363042
    25. Enhanced expression of Annexin A4 is associated with clear cell carcinoma of the ovary PMID: 19598262

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  • 亞細(xì)胞定位：
    Zymogen granule membrane; Peripheral membrane protein.
  • 蛋白家族：
    Annexin family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 542

    OMIM: 106491

    KEGG: hsa:307

    STRING: 9606.ENSP00000377833

    UniGene: Hs.422986