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  4. DBF4 Recombinant Monoclonal Antibody

DBF4 Recombinant Monoclonal Antibody

  • 中文名稱：
    DBF4重組抗體
  • 貨號：
    CSB-RA155202A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: PC3 whole cell lysate, Hela whole cell lysate, 293T whole cell lysate, HepG2 whole cell lysate, U937 whole cell lysate
      All lanes: DBF4 antibody at 1:2000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 77, 27 kDa
      Observed band size: 72-95 kDa
    • Immunofluorescence staining of A549 cell with CSB-RA155202A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 551-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    CSB-RA155202A0HU重組單克隆抗體是針對DBF4蛋白的高特異性科研工具，適用于ELISA、Western Blot（WB）和免疫熒光（IF）檢測。DBF4作為DNA復(fù)制起始的關(guān)鍵調(diào)控因子，通過與CDC7激酶形成復(fù)合物激活其激酶活性，在細(xì)胞周期S期啟動、DNA復(fù)制檢驗(yàn)點(diǎn)調(diào)控及基因組穩(wěn)定性維持中發(fā)揮重要作用。經(jīng)多平臺驗(yàn)證，該抗體在WB實(shí)驗(yàn)中可檢測到目標(biāo)蛋白的清晰特異性條帶，推薦使用稀釋度為1:500-1:2000；在IF實(shí)驗(yàn)中能精準(zhǔn)定位細(xì)胞內(nèi)靶蛋白，推薦稀釋度1:50-1:200，其嚴(yán)格的質(zhì)控標(biāo)準(zhǔn)確保了實(shí)驗(yàn)結(jié)果的可靠性和重復(fù)性。該產(chǎn)品適用于研究DNA復(fù)制調(diào)控機(jī)制、細(xì)胞周期異常相關(guān)疾?。ㄈ缒[瘤發(fā)生發(fā)展）以及基因組穩(wěn)定性相關(guān)信號通路，為分子生物學(xué)、細(xì)胞生物學(xué)等基礎(chǔ)研究領(lǐng)域提供高效工具。所有實(shí)驗(yàn)數(shù)據(jù)均經(jīng)陽性和陰性對照驗(yàn)證，滿足科研實(shí)驗(yàn)對特異性、靈敏度及穩(wěn)定性的高標(biāo)準(zhǔn)需求。
  • Uniprot No.：
  • 基因名：
  • 別名：
    Protein DBF4 homolog A (Activator of S phase kinase) (Chiffon homolog A) (DBF4-type zinc finger-containing protein 1), DBF4, ASK DBF4A ZDBF1
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from human DBF4
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    24D8
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IF
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IF 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.
  • 基因功能參考文獻(xiàn)：
    1. Both CDC7 and DBF4 promoters bind E2F, suggesting that increased E2F activity in RB1 mutant cancers promotes increased DDK expression. Surprisingly, increased DDK expression levels are also correlated with both increased chemoresistance and genome-wide mutation frequencies. PMID: 28448802
    2. Histone H3 lysine 9 (H3K9) acetylation was most prevalent when the Dbf4 transcription level was highest whereas the H3K9me3 level was greatest during and just after replication. PMID: 27341472
    3. we propose that phosphorylation of TOP2A by CDC7/DBF4 in early S-phase prevents its localization and/or activity at centromeres, and inhibition of TOP2A function could be relevant to prevent premature separation of centromeric DNA. PMID: 27407105
    4. The anti-invasive and cell cycle arrest-inducing effects of nitrogen-containing bisphosphonates are not DBF4 mediated in human breast cancer cells. PMID: 24287290
    5. Dbf4 is direct downstream target of ataxia telangiectasia mutated (ATM) and ataxia telangiectasia and Rad3-related (ATR) protein to regulate intra-S-phase checkpoint. PMID: 22123827
    6. bipartite interaction between Cdc7 and Dbf4/ASK subunits facilitates ATP binding and substrate recognition by the Cdc7 kinase. PMID: 21536671
    7. Dbf4 regulates the Cdc5 Polo-like kinase through a distinct non-canonical binding interaction PMID: 21036905
    8. Data document the role of DBF4 as a key player in nitrogen-containing bisphosphonate-induced cytotoxicity, thus explaining the effects on the cell-cycle. PMID: 19744312
    9. The interaction with LEDGF relieves autoinhibition of Cdc7-ASK kinase, imposed by the C terminus of ASK. PMID: 19864417
    10. results suggest that E2F regulates the ASK promoter through an atypical mode of recognition of the target site PMID: 12015319
    11. The HuDbf4 gene has of 12 exons over a 33-kb region. The MluI cell-cycle box is needed for core promoter activation. The Sp1 upregulator & HES-1 repressor coordinately determine promoter efficiency. Transcription initiations occur at -220, -235 and -245. PMID: 12420215
    12. Results demonstrate a DNA damage checkpoint that targets Cdc7/Dbf4 protein kinase. PMID: 12535533
    13. Cdc7-Dbf4 kinase efficiently phosphorylates p150. PMID: 16826239
    14. Taken together, these results indicate that Cdc7/Dbf4 phosphorylation of MCM2 is essential for the initiation of DNA replication in mammalian cells. PMID: 16899510
    15. Overexpression of Dbf4 abrogates the S checkpoint response to ultraviolet radiation but not ionizing radiation. PMID: 17276990
    16. There is the differential regulation of a novel gene, namely ASK/Dbf4, in melanoma and suggest that up-regulation of ASK/Dbf4 is a novel molecular determinant with prognostic relevance that confers a proliferative advantage in cutaneous melanoma. PMID: 17768177
    17. Cdc7/Dbf4 kinase activity inhibition affects specific phosphorylation sites on MCM2 in cancer cells PMID: 18286467
    18. ASK/Dbf4, a novel cell survival gene in melanoma is transcriptionally regulated by E2F1. PMID: 18503552
    19. A strong origin of replication at the DBF4 promoter locus, which contains two initiation zones, two origin recognition complex (ORC) binding sites and two DNase I-hypersensitive regions, is identified. PMID: 18536724
    20. increased Cdc7-Dbf4 abundance may be a common occurrence in human malignancies PMID: 18714392
    21. These results indicate that Ddk functions as an upstream regulator to monitor S-phase checkpoint signaling. PMID: 19111665

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  • 亞細(xì)胞定位：
    Nucleus.
  • 組織特異性：
    Highly expressed in testis and thymus. Expressed also in most cancer cells lines.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 17364

    OMIM: 604281

    KEGG: hsa:10926

    STRING: 9606.ENSP00000265728

    UniGene: Hs.485380