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DYNLL1 Recombinant Monoclonal Antibody

  • 中文名稱(chēng)：
    DYNLL1重組抗體
  • 貨號(hào)：
    CSB-RA150369A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • IHC image of CSB-RA150369A0HU diluted at 1:50 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.58% DAB.
    • Overlay Peak curve showing MCF-7 cells stained with CSB-RA150369A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    CSB-RA150369A0HU DYNLL1重組單克隆抗體是針對(duì)動(dòng)力輕鏈蛋白1（DYNLL1/LCS1）研發(fā)的高特異性檢測(cè)工具。該抗體靶向的DYNLL1作為動(dòng)力蛋白家族核心調(diào)控亞基，通過(guò)介導(dǎo)微管網(wǎng)絡(luò)動(dòng)態(tài)重組參與細(xì)胞內(nèi)物質(zhì)運(yùn)輸、有絲分裂調(diào)控及凋亡信號(hào)傳導(dǎo)，其表達(dá)異常與腫瘤發(fā)生發(fā)展、神經(jīng)退行性疾病進(jìn)程密切相關(guān)。經(jīng)多平臺(tái)驗(yàn)證顯示，該抗體在ELISA實(shí)驗(yàn)中展現(xiàn)優(yōu)異線(xiàn)性檢測(cè)性能，免疫組化（IHC）可清晰定位組織切片中DYNLL1的亞細(xì)胞分布特征，流式細(xì)胞術(shù)（FC）則能有效區(qū)分不同處理?xiàng)l件下細(xì)胞表面及胞內(nèi)蛋白表達(dá)差異，推薦使用濃度分別為IHC 1:50-200、FC 1:50-200。適用于探究DYNLL1在腫瘤微環(huán)境調(diào)控、神經(jīng)元軸突運(yùn)輸障礙、細(xì)胞周期異常等領(lǐng)域的分子機(jī)制研究，尤其可為癌癥生物學(xué)模型構(gòu)建、神經(jīng)退行性疾病相關(guān)蛋白互作網(wǎng)絡(luò)解析以及藥物靶點(diǎn)篩選提供可靠檢測(cè)方案?；蚬こ讨亟M技術(shù)保障了抗體批次間的高度一致性，滿(mǎn)足細(xì)胞成像、蛋白質(zhì)定位及定量分析等精細(xì)化研究需求。
  • Uniprot No.：
  • 基因名：
    DYNLL1
  • 別名：
    Dynein light chain 1, cytoplasmic (8 kDa dynein light chain) (DLC8) (Dynein light chain LC8-type 1) (Protein inhibitor of neuronal nitric oxide synthase) (PIN), DYNLL1, DLC1 DNCL1 DNCLC1 HDLC1
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from Human DYNLL1
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類(lèi)型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號(hào)：
    20A1
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, IHC, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    IHC 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in changing or maintaining the spatial distribution of cytoskeletal structures.; Binds and inhibits the catalytic activity of neuronal nitric oxide synthase.; Promotes transactivation functions of ESR1 and plays a role in the nuclear localization of ESR1.; Regulates apoptotic activities of BCL2L11 by sequestering it to microtubules. Upon apoptotic stimuli the BCL2L11-DYNLL1 complex dissociates from cytoplasmic dynein and translocates to mitochondria and sequesters BCL2 thus neutralizing its antiapoptotic activity.
  • 基因功能參考文獻(xiàn)：
    1. It has been found that APC2 localizes as distinct clusters along microtubule bundles in dendrites, and that this localization is driven by LC8-binding and two separate microtubule-interacting domains. PMID: 30018294
    2. High PIN expression is associated with Dilated Hearts. PMID: 27481317
    3. An expanded list of LC8 binding partners revealed the evolutionary plasticity of binding partners despite the highly conserved binding interface. In addition, it also highlighted a novel, conserved function of LC8 in the upstream regulation of the Hippo signaling pathway. PMID: 29240760
    4. DLC1 binding to nNOS-calmodulin complex does not affect the electron transport from the reductase to the oxygenase domain. PMID: 26923072
    5. NMR-derived secondary chemical shifts and relaxation properties show that the Chica LC8 binding domain is essentially disordered with a dynamically restricted segment in one linker between motifs. PMID: 26652654
    6. Studies indicate that dynein light chain LC8 has been termed an intrinsically disordered proteins (IDPs) dimerization 'hub' protein. PMID: 26226419
    7. DLC1 binding motif in L is involved in cytoskeleton localization and reorganization, primary transcription regulation by DLC1, and regulation of cellular DLC1 gene expression. PMID: 26157129
    8. The dynein light intermediate chain has a Ras-like fold with insertions that distinguish it from Ras and other previously described G proteins. PMID: 25272277
    9. Authors demonstrate that the interaction between ebola virus VP35 and dynein LC8 is direct and of high affinity and that binding stabilizes the VP35 N-terminal oligomerization domain and enhances viral RNA synthesis. PMID: 25741013
    10. Overall, this study demonstrates the novel interaction between HIV-1 integrase and cellular DYNLL1 proteins and suggests the requirement of this virus-cell interaction for proper uncoating and efficient reverse transcription of HIV-1. PMID: 25568209
    11. Structural analysis of LC8 with both Nek9 peptides, together with different biophysical experiments, explains the observed diminished binding affinity of Nek9 to LC8 upon phosphorylation on Ser(944) within the Nek9 sequence PMID: 23482567
    12. Dynein forms distinct complexes requiring specific recruiters and activators to promote orderly progression through mitosis. PMID: 23589491
    13. Overexpressed human LC8 inhibits mouse osteoclast differentiation by regulating NF-kappaB & MAPK pathways and suppressing RANKL signaling. PMID: 23293355
    14. DYNLL1 interacted with a spindle-microtubule-associated adaptor formed by CHICA and HMMR via TQT motifs in CHICA. PMID: 22965910
    15. appropriate levels of ternary complex components are critical for dynein-dependent spindle positioning in HeLa cells and C. elegans embryos PMID: 23027904
    16. Cytosolic mfGbeta is recruited to dynein by Nudel and transported to the centrosome for rapid sequestration and degradation. PMID: 22430153
    17. Study found the secretion of calu-1/2-EGFP required microtubule integrity, and that calu-1/2-EGFP-containing vesicles were transported by the motor proteins Kif5b and cytoplasmic dynein. PMID: 22514732
    18. The ASCIZ-DYNLL1 feedback loop represents a novel mechanism for auto-regulation of gene expression, where the gene product directly inhibits the transcriptional activator while bound at its own promoter. PMID: 22167198
    19. The study uses thermodynamics and dynamics measurements of LC8 complexes to group LC8 binding partners in two categories: those whose binding is enthalpically driven and those that are entropically favored. PMID: 21621319
    20. these results imply a potential cellular interference between DYNLL1 and ATMIN functions. PMID: 21971545
    21. results significantly widen the scope of the human interactome around DYNLL and will certainly shed more light on the biological functions and organizing role of DYNLL in the human and other eukaryotic interactomes PMID: 21533121
    22. DYNLL/LC8 protein controls signal transduction through the Nek9/Nek6 signaling module by regulating Nek6 binding to Nek9. PMID: 21454704
    23. Data demonstrate the direct interaction between DYNLL1 and peptides derived from ASFV p54 and gephyrin interacting sequences. PMID: 21094642
    24. effect of glycosylation on protein folding thermodynamics and kinetics PMID: 20936810
    25. Data suggest that MTOC-directed movement of lytic granules in NK cells is independent of actin and microtubule reorganization, dependent on dynein motor function, occurs before MTOC polarization, and does not require a commitment to cytotoxicity. PMID: 20444980
    26. the thermodynamic and kinetic fine-tuning of binding of various ligands to DYNLL could have physiological relevance in its interaction network. PMID: 20889982
    27. Data provide the first trafficking regulatory role for Crmp2 in non-neuronal cells and support a model in which Crmp2 is an important endocytic regulatory protein that links MICAL-L1.EHD1-based vesicular transport to dynein motors. PMID: 20801876
    28. Data identify GRINL1A as a membrane-associated DYNLL1 binding partner and suggest that additional DYNLL1-binding partners are present near this glutamate channel homolog. PMID: 20412299
    29. the regulation of DLC1 by p21-activated kinase 1 is a novel mechanism by which a signaling kinase might influence macropinocytosis PMID: 15504720
    30. LC8 binds to p53-binding protein 1 and mediates DNA damage-induced p53 nuclear accumulation PMID: 15611139
    31. PAX6 interacts with HOMER3, DNCL1, and TRIM11. Three C-terminal PAX6 mutations, previously identified in patients with eye malformations, all reduced or abolished the interactions. PMID: 16098226
    32. the DLC1-KIBRA interaction is essential for ER transactivation in breast cancer cells PMID: 16684779
    33. PIN not only inhibits nitric oxide but also .O(2)(-) production from nitric oxide synthase 1 PMID: 16781079
    34. dynein light chain 1 represents a novel anchoring protein for RasGRP3 that may regulate subcellular localization of the exchange factor PMID: 17012239
    35. we established for the first time expression of PIN/LC8 in human mast cells PMID: 17169380
    36. findings reveal a previously unrecognized regulatory mechanism of DLC1 in which the Ser(88) phosphorylation acts as a molecular switch for the transition of DLC1 from dimer to monomer PMID: 18084006
    37. LC8 binds IkappaBalpha in a redox-dependent manner and thereby prevents its phosphorylation by IKK. TRP14 contributes to this inhibitory activity by maintaining LC8 in a reduced state. PMID: 18579519
    38. pH dependent unfolding characteristics of DLC8 dimer: Residue level details from NMR. PMID: 18692162
    39. The data indicated the presence of intermediates along the equilibrium unfolding transition; the hydrogen exchange data suggested that the DLC8 molecule has differential stability in its various segments. PMID: 18767155
    40. Hierarchy in guanidine unfolding of DYNLL1 dimer is reported. PMID: 19032974
    41. LC8 facilitates nuclear import of Pak1 and this function is indispensable during vertebrate development PMID: 19557173

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  • 亞細(xì)胞定位：
    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton. Nucleus. Mitochondrion.
  • 蛋白家族：
    Dynein light chain family
  • 組織特異性：
    Ubiquitous. Expressed in testis.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 15476

    OMIM: 601562

    KEGG: hsa:8655

    STRING: 9606.ENSP00000242577

    UniGene: Hs.5120