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EEF2 Recombinant Monoclonal Antibody

  • 中文名稱(chēng)：
    EEF2 Recombinant Monoclonal Antibody
  • 貨號(hào)：
    CSB-RA053731A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: THP-1 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate(30μg), HEK293 whole cell lysate, NIH/3T3 whole cell lysate, HeLa whole cell lysate(30μg)
      All lanes: eEF2 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 95 kDa
      Observed band size: 115 kDa
      Exposure time: 10s
    • Immunofluorescence staining of Jurkat cell with CSB-RA053731A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Overlay Peak curve showing jurkat cells stained with CSB-RA053731A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產(chǎn)品詳情

  • Uniprot No.：
  • 基因名：
  • 別名：
    EEF 2 antibody; Eef2 antibody; EF-2 antibody; EF2 antibody; EF2_HUMAN antibody; Elongation factor 2 antibody; Eukaryotic translation elongation factor 2 antibody; Polypeptidyl tRNA translocase antibody; SCA26 antibody
  • 反應(yīng)種屬：
    Human, Mouse
  • 免疫原：
    A synthesized peptide derived from human EEF2
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類(lèi)型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號(hào)：
    8B4
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IF, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
  • 基因功能參考文獻(xiàn)：
    1. our study indicates that expression of eEF2 protein is a potential biomarker for evaluating prostate cancer PMID: 28913610
    2. eEF2 may be activated in a positive feedback cycle through inactivation of eEF2K via the PI3K/Akt/mTOR pathway. PMID: 30060827
    3. the direct interaction of AKT2 and EF2 was found to be dynamically regulated in embryonic rat cardiomyocytes PMID: 23823123
    4. These results suggest that binding of eEF2 to the ribosome alters its conformation, resulting in a weakened affinity of eIF5A and impairment of this interplay compromises cell growth due to translation elongation defects. PMID: 27115996
    5. nuclear localization of active eEF-2 depends upon its interaction with p53, as cells lacking p53 contain less active eEF-2 in the nuclear compartment PMID: 23542375
    6. High serum eukaryotic elongation factor 2 level is associated with non-small cell lung cancer. PMID: 24377563
    7. Results indicated that the eEF2 gene is overexpressed in the majority of several types of cancers and plays an oncogenic role in cancer cell growth. PMID: 24589652
    8. The expression levels of three putative HA-regulated proteins (TALDO, ANXA1 and EF2) in control, H2O2-, HA- and HA/H2O2-treated OA chondrocytes were verified by Western blotting and the results indeed support the notion that HA acts in anti-oxidation PMID: 24480285
    9. Thiopental inhibits global protein synthesis by repression of eukaryotic elongation factor 2 and protects from hypoxic neuronal cell death. PMID: 24167567
    10. A single amino acid substitution in eukaryotic elongation factor 2 co-segregates with the disease phenotype of spinocerebellar ataxia 26. PMID: 23001565
    11. EEF2 phosphorylation by cyclin A-cyclin-dependent kinase 2 (CDK2) on a novel site, serine 595 (S595), directly regulates T56 phosphorylation by eEF2K. PMID: 23184662
    12. Burn induces prolonged activation of eEF2K and eEF2 in pediatric patients. PMID: 22269896
    13. PHD2 modulated eEF2 activity and protein translation under acute hypoxia. PMID: 22308030
    14. NH125 induces eEF2 phosphorylation (peEF2) through multiple pathways in cancer cells PMID: 22020937
    15. Training in the fasted state, compared with identical training with ample carbohydrate intake, facilitates post-exercise dephosphorylation of eEF2. PMID: 21132439
    16. results suggest that eEF2 is an anti-apoptotic marker in lung adenocarcinoma PMID: 21554491
    17. eEF-2 is activated by both lithium and GSK-3, whereas, lithium treatment and inhibition of GSK-3 have opposing effects on eEF-2. PMID: 20708687
    18. melanoma antigen expressed in G361, a representative melanoma cell line/ reacted with autoantibodies in patient sera PMID: 20181627
    19. inhibition of protein synthesis under high pressure occurs independent of the phosphorylation of eEF-2 PMID: 15534876
    20. Levels of p-eEF2K were significantly increased, and total eEF2 significantly decreased in Alzheimer disease PMID: 16098202
    21. Data show that overproduction of elongation factor 2 blocks HIV-1 viral protein R-induced cell death both in fission yeast and human cells. PMID: 16520893
    22. Hypoxia inhibits protein synthesis through a 4EBP1 and EEF2 pathway controlled by mTOR and unbcoupled in breast cancer cells. PMID: 16648488
    23. Skeletal muscle eEF2 and 4EBP1 phosphorylation during endurance exercise is dependent on intensity and muscle fiber type. PMID: 19036825
    24. Overexpressed eEF2 in gastrointestinal cancers promoted G2/M progression and enhanced their cell growth in vitro and in vivo. PMID: 19360331

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  • 相關(guān)疾病：
    Spinocerebellar ataxia 26 (SCA26)
  • 亞細(xì)胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    TRAFAC class translation factor GTPase superfamily, Classic translation factor GTPase family, EF-G/EF-2 subfamily
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 3214

    OMIM: 130610

    KEGG: hsa:1938

    STRING: 9606.ENSP00000307940

    UniGene: Hs.515070