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FTH1 Recombinant Monoclonal Antibody

  • 中文名稱：
    FTH1重組抗體
  • 貨號：
    CSB-RA574579A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: Mouse Liver tissue lysate
      All lanes:Ferritin antibody at 1:500
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 21 kDa
      Observed band size: 21 kDa
    • Immunofluorescence staining of HepG2 with CSB-RA574579A0HU at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 497-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Overlay Peak curve showing 293 cells stained with CSB-RA574579A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產品詳情

  • 產品描述：
    CSB-RA574579A0HU FTH1重組單克隆抗體是針對鐵蛋白重鏈（Ferritin Heavy Chain, FTH1）開發(fā)的高特異性科研試劑。FTH1是鐵代謝關鍵蛋白，通過結合并儲存游離鐵離子參與細胞內鐵穩(wěn)態(tài)調控，在抗氧化應激、炎癥反應及細胞保護中發(fā)揮重要作用。本抗體采用重組技術制備，具有高批次一致性和低交叉反應性，經嚴格驗證可在多種實驗場景中穩(wěn)定應用：Western Blot（WB）中可清晰檢測目標蛋白表達，推薦稀釋度1:500-1:2000；免疫熒光（IF）實驗顯示特異性定位，推薦稀釋度1:50-1:200；流式細胞術（FC）驗證其適用于細胞表面及胞內靶標檢測，推薦稀釋度1:50-1:200；同時支持ELISA平臺定量分析。該產品適用于鐵代謝調控機制、氧化應激相關通路研究，以及神經退行性疾病、貧血、腫瘤微環(huán)境等領域的體外模型研究，為探索FTH1在鐵依賴性細胞死亡（ferroptosis）、自由基清除等生理病理過程中的功能提供可靠工具。所有數(shù)據(jù)均經陽性和陰性對照驗證，確保實驗結果的可重復性，嚴格限于科學研究使用。
  • Uniprot No.：
  • 基因名：
  • 別名：
    Ferritin heavy chain (Ferritin H subunit) (EC 1.16.3.1) (Cell proliferation-inducing gene 15 protein) [Cleaved into: Ferritin heavy chain, N-terminally processed], FTH1, FTH FTHL6
  • 反應種屬：
    Human, Mouse
  • 免疫原：
    A synthesized peptide derived from Human FTH1
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    39G3
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IF, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.
  • 基因功能參考文獻：
    1. Human oligodendrocytes undergo apoptosis when exposed to Sema4A and take up H-ferritin for meeting iron requirements and that these functions are mediated via the Tim-1 receptor. H-ferritin can block Sema4A-mediated cytotoxicity. Sema4A is detectable in the CSF of multiple sclerosis patients and HIV-seropositive persons and can induce oligodendrocyte cell death. PMID: 29457657
    2. High serum ferritin (SF) and the overall survival of allogeneic hematopoietic SF was only significantly associated with prolonged RBC transfusion dependence post-transplantation. PMID: 29046127
    3. the role of FTH1 in the FIH control of HIF-1 activity, is reported. PMID: 29580991
    4. Study demonstrate that the amounts of ferritin heavy subunit are inversely correlated, in K562 cells, to H19/miR-675 levels, and that this phenomenon may be largely attributed to the increase in ROS production induced by the FHC silencing. PMID: 29544765
    5. Findings indicate that induction of EMT, increased migration and survival depend, in MCF-7 and H460 cells, on the release of FHC control on two pathways, namely the iron/ROS metabolism and CXCR4/CXCL12 axis. PMID: 28774348
    6. ferritin heavy chain has a role in ovarian cancer stem cell expansion and epithelial to mesenchymal transition PMID: 27566559
    7. Report in vivo magnetic resonance imaging of xenografted neuroblastoma tumors using FTH1 reporter gene expression controlled by a tet-on switch. PMID: 27732930
    8. The H-ferritin could bind up to 24 NCOA4(383-522) fragments forming highly stable and insoluble complexes. The binding was partially inhibited only by Fe(II) among the various divalent metal ions analyzed. The iron-dependent, highly-specific formation of the remarkably stable H-ferritin-NCOA4 complex shown in this work may be important for the characterization of the mechanism of ferritinophagy. PMID: 28754384
    9. Recombinant human H-chain ferritin nanocages were characterized by transmission electron microscopy and dynamic light scattering. These results indicate that H-chain ferritin is able to self-assemble into nanocages with a narrow size distribution. PMID: 26853188
    10. Among myelodysplastic syndromes patients only, CD163 + macrophage density and HO1 and H-ferritin expression by CD163 + macrophages increased in tandem with marrow iron. High HO1 was significantly associated with shorter overall survival. PMID: 26758041
    11. Low levels of FTH-positive tumor cells and microglia/macrophages were associated with poor survival in anaplastic astrocytomas, while high amounts of FTL-positive microglia/macrophages had a negative prognostic value PMID: 28837569
    12. there is no significant relationship between serum ferritin concentrations and depressive symptoms among Chinese adults. PMID: 27611581
    13. Data suggest that, in homopolymeric H-subunit ferritin (HuHF), iron oxidation proceeds with a 2:1 Fe(II):O(2) stoichiometry at iron level of 2 Fe(II) atoms/H-subunit with generation of H2O2; L-subunit-rich heteropolymeric ferritin also facilitates iron oxidation at the ferroxidase center and additionally promotes oxidation at the mineral surface once iron-binding capacity has been exceeded. PMID: 28636371
    14. Elevated serum ferritin levels are associated with hematologic malignancies. PMID: 25124580
    15. Ferritin light chain and ferritin heavy chain are required for the neural differentiation of bone marrow-derived mesenchymal stem cells under extremely low-frequency electromagnetic field. PMID: 26602884
    16. Human FTH1 is a general pro-survival sequence. PMID: 26886577
    17. H-ferritin tissue expression and the number of CD68+/H-ferritin+ cells were increased in the lymph nodes of adult-onset Still's disease patients, and these results correlated significantly with disease severity PMID: 26540556
    18. Stopped-flow kinetics assays revealed that human H ferritin has different levels of activity compared with its R. catesbeiana counterpart PMID: 26327381
    19. Studies indicate that the the best characterized cytosolic ferritins in mammals are encoded by two genes, FTH and FTL, with four exons and similar structures. PMID: 26518749
    20. high cytoplasmic FTH1 was associated with favorable prognosis whereas nuclear FTH1 staining was associated with adverse prognosis in triple negative breast cancer PMID: 24742827
    21. The goals of this study were to evaluate the multilineage differentiation ability of human mesenchymal stem cells expressing an MRI reporter, human ferritin heavy chain (FTH). PMID: 25448225
    22. Data suggest that iron-independent effects of ferritin in myeloma should be prospectively investigated in preclinical and clinical studies. PMID: 24512320
    23. Significant differences were seen in the levels of serum iron and hepcidin but not ferritin between the -582A>G hepcidin polymorphism genotypes. PMID: 25134646
    24. High ferritin expression is associated with malignant phenotype of breast cancer. PMID: 25265351
    25. High serum ferritin levels are associated with pancreatic cancer. PMID: 24446242
    26. FTH provides metabolic adaptation to tissue Fe overload. [Review] PMID: 24124891
    27. Tumor necrosis factor-alpha attenuates starvation-induced apoptosis through upregulation of ferritin heavy chain in hepatocellular carcinoma cells. PMID: 24066693
    28. Elevated serum ferritin level is associated with the incident type 2 diabetes in healthy Korean men. PMID: 24098686
    29. Children with autism spectrum disorders have significantly lower ferritin levels compared with controls and higher levels of sleep fragmentation and periodic limb movements. PMID: 24053984
    30. Data indicate that serum highest TNF-alpha and IL-6 and higher ferritin are among those with intense steatosis. PMID: 23892696
    31. Traumatic brain injury upregulates ferritin-heavy-chain in pericontusional cortex. PMID: 23079850
    32. Increased levels of serum ferritin are adversely associated with cardiac function following myocardial infarction treated with percutaneous coronary intervention. PMID: 23079089
    33. In normal bone marrow, ferritin H antibody stained early erythroid precursors and macrophages. In leukemic bone marrow, ferritin H was selectively expressed in erythroid blasts (M6), whereas all other blasts were negative. PMID: 23611361
    34. The present study provides information about the metal binding sites of human H-chain ferritin and the pathway of ferrous ions (Fe2+) from the cytoplasm to the interior of the ferritin nanocage. PMID: 23344859
    35. Serum ferritin levels in infectious diseases was lower than those in hematologic diseases. PMID: 23324584
    36. Data indicate that ferritin levels and hyperuricemia are two independently significant factors associated with alanine aminotransferase (ALT) elevation among obese adolescents. PMID: 23119080
    37. Increased levels of ferritin and decreased total antioxidant status indicated increasing inflammation in chronic hepatitis C. PMID: 22760008
    38. H-ferritin overexpression promotes radiation-induced leukemia/lymphoma. PMID: 22843505
    39. The hepcidin:ferritin ratio is reduced in relation to increasing fibrosis in chronic liver disease. PMID: 22676252
    40. To study whether the human ferritin heavy chain (FTH1) can be expressed in Hansenula polymorpha, we integrated an expression cassette for FTH1, analyzed the protein expression, and also designed a FTH1-PTH fusion protein. PMID: 22212821
    41. Induction of ferritin heavy chain expression results in severe inhibition of apoB-100 secretion from hepatoma cells during HCV infection. PMID: 22443280
    42. Survivin inhibition by an interacting recombinant peptide, derived from the human ferritin heavy chain, impedes tumor cell growth PMID: 22426960
    43. Elevated serum ferrin in infants is associated with congenital hypothyroidism. PMID: 22768652
    44. Data provide useful insights into biomineralization of ferritin under in vivo fever conditions as well as in biomimetic synthesis of nanomaterials using ferritin. PMID: 22020807
    45. FTH1 can inhibit the activation of the JNK signaling pathway and can bind to Daxx, inhibiting Daxx-mediated apoptosis. PMID: 21573799
    46. H ferritin silenced human metastatic melanoma cells are characterized by a decreased growth activity, a reduced invasiveness, and a reduced cell adhesion capability. PMID: 22043922
    47. There was a positive correlation between reactive oxygen species levels and serum ferritin levels in myelodysplastic syndrome patients PMID: 22117997
    48. results showed the association of secreted IL-10, FHC and iron homoeostasis in Chlamydia trachomatis-infected HeLa-229 cells PMID: 21413929
    49. FTH1 is a functional target gene of the BACH1 transcription factor according to ChIP-seq and knockdown analysis in HEK 293 cells. PMID: 21555518
    50. Results illustrate how silencing H-ferritin can effectively sensitize tumors to chemotherapy. PMID: 21385903

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  • 相關疾?。?/div>
    Hemochromatosis 5 (HFE5)
  • 蛋白家族：
    Ferritin family
  • 組織特異性：
    Expressed in the liver.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 3976

    OMIM: 134770

    KEGG: hsa:2495

    STRING: 9606.ENSP00000273550

    UniGene: Hs.524910