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ILDR2 Recombinant Monoclonal Antibody

  • 中文名稱：
    ILDR2 Recombinant Monoclonal Antibody
  • 貨號(hào)：
    CSB-RA751251MA1HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Immunofluorescence?staining?of?Hela?cell?with?CSB-RA751251MA1HU?at?1:30,?counter-stained?with?DAPI.?The?cells?were?fixed?in?4%?formaldehyde?and?blocked?in?10%?normal?Goat?Serum.?The?cells?were?then?incubated?with?the?antibody?overnight?at?4C.?The?secondary?antibody?was?FITC-conjugated?AffiniPure?Goat?Anti-Human?IgG(H+L).
    • Immunofluorescence?staining?of U251?cell?with?CSB-RA751251MA1HU?at?1:30,?counter-stained?with?DAPI.?The?cells?were?fixed?in?4%?formaldehyde?and?blocked?in?10%?normal?Goat?Serum.?The?cells?were?then?incubated?with?the?antibody?overnight?at?4C.?The?secondary?antibody?was?FITC-conjugated?AffiniPure?Goat?Anti-Human?IgG(H+L).
    • Overlay Peak curve showing Jurkat cells stained with CSB-RA751251MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100.Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was human IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    CSB-RA751251MA1HU ILDR2重組單克隆抗體是經(jīng)ELISA、免疫熒光（IF）和流式細(xì)胞術(shù)（FC）嚴(yán)格驗(yàn)證的高特異性科研工具。該抗體靶向ILDR2蛋白，該分子作為免疫球蛋白超家族成員，在細(xì)胞間連接復(fù)合體形成及上皮極性建立中發(fā)揮重要作用，與組織屏障功能調(diào)控密切相關(guān)。實(shí)驗(yàn)數(shù)據(jù)顯示，該抗體在IF應(yīng)用中能清晰呈現(xiàn)細(xì)胞膜或特定亞細(xì)胞結(jié)構(gòu)的定位信號(hào)，推薦使用稀釋比例為1:50-1:200；在FC檢測中可有效識(shí)別天然構(gòu)象的靶蛋白，推薦同等稀釋范圍。經(jīng)ELISA驗(yàn)證其具備優(yōu)異的目標(biāo)蛋白結(jié)合能力，適用于體外蛋白互作分析。本產(chǎn)品可廣泛應(yīng)用于細(xì)胞連接機(jī)制研究、上皮組織發(fā)育模型構(gòu)建，以及相關(guān)信號(hào)通路的功能解析等基礎(chǔ)研究領(lǐng)域，為探索ILDR2在細(xì)胞極性調(diào)控和生理病理過程中的分子機(jī)制提供可靠支持。
  • Uniprot No.：
  • 基因名：
  • 別名：
    ILDR2; C1orf32Immunoglobulin-like domain-containing receptor 2
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    Recombinant Human ILDR2 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    hIgG1
  • 純化方式：
    Affinity-chromatography
  • 克隆號(hào)：
    13G7
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, IF, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    May be involved in lipid homeostasis and ER stress pathways.
  • 基因功能參考文獻(xiàn)：
    1. We demonstrated that ZNF70 interacts with ZFP64 and activates HES1 transcription by binding to the HES1 promoter. In addition, HES1 gene expression is increased in ILDR2-knockdown HepG2 cells, in which ZNF70 is translocated from the cytoplasm to the nucleus, suggesting that ZNF70 migration to the nucleus after dissociating from the ILDR2-ZNF70 complex activates HES1 transcription. These results support a novel link betwee PMID: 27353377
    2. Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 20379614
    3. Observational study of gene-disease association. (HuGE Navigator) PMID: 19536175
    4. Observational study of gene-disease association. (HuGE Navigator) PMID: 19064610
  • 亞細(xì)胞定位：
    Endoplasmic reticulum membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Immunoglobulin superfamily, LISCH7 family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 18131

    KEGG: hsa:387597

    UniGene: Hs.444835