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  4. KLC3 Recombinant Monoclonal Antibody

KLC3 Recombinant Monoclonal Antibody

  • 中文名稱：
    KLC3 Recombinant Monoclonal Antibody
  • 貨號(hào)：
    CSB-RA889602A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: HepG2 whole cell lysate(20μg),COLO-205 whole cell lysate(20μg),Jurkat whole cell lysate(20μg), A549 whole cell lysate(20μg),Hela whole cell lysate(20μg),MCF-7 whole cell lysate(20μg),Mouse skin tissue lysate(20μg)
      All lanes: Kinesin light chain 3 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 55 kDa
      Observed band size: 57 kDa
      Exposure time：2s
    • IHC image of CSB-RA889602A0HU diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA889602A0HU diluted at 1:100 and staining in paraffin-embedded human skin tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Overlay Peak curve showing Hela cells stained with CSB-RA889602A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產(chǎn)品詳情

  • Uniprot No.：
  • 基因名：
    KLC3
  • 別名：
    Kinesin light chain 2 antibody; Kinesin light chain 3 antibody; KLC2 antibody; KLC2 like antibody; KLC2-like antibody; KLC2L antibody; KLC3 antibody; KLC3_HUMAN antibody; KLCt antibody; KNS2B antibody
  • 反應(yīng)種屬：
    Human, Mouse
  • 免疫原：
    A synthesized peptide derived from human KLC3
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號(hào)：
    14D4
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IHC, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport.
  • 基因功能參考文獻(xiàn)：
    1. Microtubule-bound kinesin-1 and kinesin-3 motor domains were visualized at multiple steps in their ATPase cycles--including their nucleotide-free states--at approximately 7 A resolution using cryo-electron microscopy. PMID: 25209998
    2. Rat KLC3 interacts with KHC and microtubules in vitro. Expressed in spleen, intestine, brain, ovary, testis. Testis expression restricted to post-meiotic spermatids. PMID: 11319135
    3. Rat KLC3 associates with the outer dense fibers of maturing sperm tails. PMID: 12594206
    4. Rat KHC3 associates with mitochondria in sperm tails PMID: 15464570
  • 亞細(xì)胞定位：
    Cytoplasm, cytoskeleton.
  • 蛋白家族：
    Kinesin light chain family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 20717

    OMIM: 601334

    KEGG: hsa:147700

    STRING: 9606.ENSP00000375810

    UniGene: Hs.298079